FlyBase Allele Report: Hsap\SOD1[UAS.cPa]

General Information

Symbol

Hsap\SOD1^UAS.cPa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Parkes

FlyBase ID

FBal0090627

Feature type

allele

Associated gene

Hsap\SOD1

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\SOD1.P}

Also Known As

UAS-hSod1, P{UAS-SOD}

Key Links

Transgenic product class

cDNA

(Parkes et al., 1998)

missense_variant

(Parkes et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Parkes et al., 1998)

missense_variant

(Parkes et al., 1998)

Mutagen

in vitro construct

(Parkes et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-Hsap\SOD1.P}

Cytology

Description

An Hsap\SOD1 cDNA is expressed under the control of UASt regulatory sequences.

(Parkes et al., 1998)

The cDNA sequence contains a missense mutation (amino acid substitution K75R).

(Parkes et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\SOD1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^RNAi.UAS.cWa

(Wang et al., 2006)

modeled by Hsap\SNCA^A30P.UAS

(Botella et al., 2008)

ameliorates amyotrophic lateral sclerosis

modeled by Sod1^X-39

(Parkes et al., 1998)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Toll-6-D42

(Petrosyan et al., 2015)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Act5C.PU

(Martin et al., 2009)

chemical resistant, with Scer\GAL4^elav.PU

(Bahadorani et al., 2013)

increased rate of movement, with Scer\GAL4^Toll-6-D42

(Petrosyan et al., 2015)

long lived, with Hsap\SOD1^G41S.UAS, Scer\GAL4^Toll-6-D42

(Elia et al., 1999)

long lived, with Scer\GAL4^Act5C.PU

(Martin et al., 2009)

long lived, with Scer\GAL4^da.G32

(Martin et al., 2009)

long lived, with Scer\GAL4^Toll-6-D42

(Parkes et al., 1998)

short lived, with Scer\GAL4^da.G32

(Bahadorani et al., 2013)

short lived, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Female flies with expression of Hsap\SOD1^Scer\UAS.cPa driven by Scer\GAL4^Toll-6-D42 maintain flight for longer durations than parental controls (but less than wild type) during most of their lifespan except the oldest age measured (50 days); male mutants are not significantly different to controls. Wingbeat frequency decreases with age (just as in controls) but is significantly lower than controls for Scer\GAL4^Toll-6-D42>Hsap\SOD1^Scer\UAS.cPa flies at older ages (both males and females). Scer\GAL4^Toll-6-D42>Hsap\SOD1^Scer\UAS.cPa flies at older ages are significantly more active than controls (especially females).

(Petrosyan et al., 2015)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 slightly shortens the life span of adults flies compared to controls.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^D42 results in a moderate decrease in lifespan.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of either Scer\GAL4^elav.PU, Scer\GAL4^repo.PU or Scer\GAL4^Mhc.PU has no major effect on longevity. Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 has no effect on mitochondrial ultrastructure in the indirect flight muscles.

Flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^elav.PU show increased resistance to paraquat compared to controls. These flies show wild-type sensitivity to zinc.

(Bahadorani et al., 2013)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^Act does not rescue the reduction in lifespan seen in flies under hyperoxia.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^ple.PF rescues the degeneration of dopaminergic neurons that is seen in flies under hyperoxia.

(Gruenewald et al., 2009)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of either Scer\GAL4^Act5C or Scer\GAL4^da.G32 increases mean life span by 15-30%. Age related impairment in negative geotaxis is modestly delayed in flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^Act5C but is not altered in flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^D42, Scer\GAL4^Appl.G1a, Scer\GAL4^elav-C155, Scer\GAL4^repo, Scer\GAL4^Mef2.PR or Scer\GAL4^how-24B does not result in an increase in lifespan.

(Martin et al., 2009)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^ple.PF suppresses the hypoxia-induced dopaminergic neuron degeneration seen in control flies.

(Botella et al., 2008)

Flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 show no alteration in lifespan compared to controls.

(Ford et al., 2007)

Simultaneous expression of both Hsap\SOD1^Scer\UAS.cPa and Hsap\SOD1^{G41S.Scer\UAS} under the control of Scer\GAL4^Toll-6-D42 significantly increases adult lifespan compared to controls.

(Elia et al., 1999)

Flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^D42 in a wild-type background have mean and maximum adult lifespans up to 40% longer than isogenic controls. The pre-mortality plateau phase of the life curve (less than 5% mortality) is extended from approximately 27 to approximately 50 days.

(Parkes et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\SOD1^UAS.cPa/Scer\GAL4^Act5C.PI is an enhancer of lethal | recessive phenotype of Trxr1^G0481

(Missirlis et al., 2001)

Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cPa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^ple.PF

(Botella et al., 2008)

Hsap\SOD1^UAS.cPa/Scer\GAL4^da.G32 is a suppressor of short lived phenotype of Ccs^29E

(Kirby et al., 2008)

Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Hsap\SOD1^UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu

(Wang et al., 2006)

Hsap\SOD1^UAS.cPa/Scer\GAL4^Mhc.PW is a suppressor of short lived phenotype of Sod1^X-39

(Missirlis et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PU/Hsap\SOD1^UAS.cPa is a non-suppressor of abnormal neurophysiology | somatic clone | adult stage | conditional phenotype of Lrpprc2^A

(Jaiswal et al., 2015)

Other

Statement

Reference

Hsap\SOD1^UAS.cPa, Scer\GAL4^Toll-6-D42, Sod1^X-39 has chemical resistant phenotype

(Parkes et al., 1998)

Hsap\SOD1^UAS.cPa, Scer\GAL4^Toll-6-D42, Sod1^X-39 has radiation resistant phenotype

(Parkes et al., 1998)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cPa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron | progressive phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^ple.PF

(Botella et al., 2008)

Hsap\SOD1^UAS.cPa/Scer\GAL4^ey.3.5.Exel is a suppressor of eye | somatic clone - Minute background phenotype of Opa1^f02779

(Yarosh et al., 2008)

Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

Hsap\SOD1^UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of dopamine neuron & adult brain phenotype of Pink1^RNAi.UAS.cWa, Scer\GAL4^elav.PLu

(Wang et al., 2006)

Hsap\SOD1^UAS.cPa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Pink1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cPa/Scer\GAL4^Mhc.PU is a non-suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Li et al., 2023)

Hsap\SOD1^UAS.cPa/Scer\GAL4^Mhc.PU is a non-suppressor of mitochondrial crista | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Hsap\SOD1^UAS.cPa/Scer\GAL4^Mhc.PU is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Hsap\SOD1^UAS.cPa/Scer\GAL4^Mhc.PU is a non-suppressor of mitochondrial membrane | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\SOD1^Scer\UAS.cPa driven by Scer\GAL4^ninaE.PU does not suppress ERG amplitude in Lrpprc2^A/Lrpprc2^A mutants (7 days old under constant light conditions).

(Jaiswal et al., 2015)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 in sicily^E mutant larvae reduces the overall level of reactive oxygen species and considerably suppresses the lipid droplet accumulation seen in these flies.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 in Aats-met^FB mutant larvae reduces the overall level of reactive oxygen species and considerably suppresses the lipid droplet accumulation seen in these flies.

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 in Marf^B mutant larvae reduces the overall level of reactive oxygen species and considerably suppresses the lipid droplet accumulation seen in these flies.

Neuronal expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^elav.PU significantly reduces lipid droplet accumulation in Marf^{ninaE.GD11094} flies.

(Liu et al., 2015)

Expression of Hsap\SOD1^Scer\UAS.cPa suppresses the dopaminergic neuron degeneration seen when Hsap\SNCA^{A30P.Scer\UAS} is expressed under the control of Scer\GAL4^ple.PF. The reduction in climbing ability is also suppressed.

(Botella et al., 2008)

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 rescues the early mortality and reduced median lifespan of Ccs^29E homozygotes.

(Kirby et al., 2008)

Scer\GAL4^ey.3.5.Exel-mediated expression of Hsap\SOD1^Scer\UAS.cPa significantly reduces the glossy eye phenotype, but not the rough eye phenotype, of opa1-like^f02779 large clones (induced in a Minute background).

(Yarosh et al., 2008)

The partial lethality (reduced eclosion rate) characteristic for Sod1^X-39 homozygotes is also almost fully rescued by expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^Toll-6-D42 in the mutant background.

(Elia et al., 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76599

w^*; P{UAS-Hsap\SOD1.P}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SOD1^Scer\UAS.cPa

Hsap\SOD1^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Parkes

(Parkes et al., 1998)

Secondary FlyBase IDs

References (18)

Report Sections

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