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Parks, A. (2004.5.12). GAL4 constructs and insertions. 
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FBrf0178842
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Personal communication to FlyBase
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The following information was provided by Annette Parks of Exelixis, Inc.
In P{ey3.5-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey3.5}.
P{ey3.5-GAL4.Exel}1 is a homozygous viable and fertile, second chromosome insertion.
P{ey3.5-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome 
insertion.
P{ey3.5-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome 
insertion.
In P{vgM-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgM}.
P{vgM-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome 
insertion.
P{vgM-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome 
insertion.
In P{ey1x-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey1x}.
P{ey1x-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome 
insertion.
P{ey1x-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome 
insertion.
In P{vgMQ-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgMQ}.
P{vgMQ-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X 
chromosome insertion.
P{vgMQ-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome 
insertion.
P{vgMQ-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome 
insertion.
P{m4-GAL4.Exel} carries GAL4 driven by the E(spl) region transcript m4 
(FBgn0002629) enhancer.  The m4 enhancer was originally PCR amplified from 
a clone described in Singson et al., 1994 (Genes Dev. 8: 2058-2071; 
FBrf0076492) and cloned into KpnI and XhoI sites in the polylinker of 
pP{Express}.  The enhancer fragment plus flanking pP{Express}sequence was 
cut out as a 0.5 kb SmaI-AscI fragment and cloned into the same sites in 
pP{Express-GAL4}.
P{m4-GAL4.Exel}2 is a second chromosome insertion.
P{E(spl)-GAL4.Exel} carries GAL4 driven by the E(spl) (FBgn0000591) 
enhancer.  The enhancer fragment was PCR amplified from a clone described 
in Lecourtois & Schweisguth, 1995 (Genes Dev. 9: 2598-2608; FBrf0084115) 
and Kramatschek & Campos-Ortega, 1994 (Development 20: 815-26; FBrf0073637) 
and cloned into KpnI and XhoI sites in the pP{Express} polylinker.  The 
enhancer fragment was cut out as a 1.1 kb SmaI-AscI fragment and cloned 
into pP{Express-GAL4}.
P{E(spl)-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X 
chromosome insertion.
P{E(spl)-GAL4.Exel}3 is a third chromosome insertion.
DOI
Related Publication(s)
Personal communication to FlyBase

P{ey3.5-GAL4.Exel}1.
Parks, 2011.6.27, P{ey3.5-GAL4.Exel}1. [FBrf0214031]

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