Tagged transgenic construct was expressed in tim[+] cells.
Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
The interaction of Clk with CkIIα was observed mostly in extracts from flies collected at the end of the subjective night and in the subjective morning.
Human-derived CK2α phosphorylates Clk in vitro, in a manner that is stimulated by per.