FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nmoc4-2.UAS
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General Information
Symbol
Dmel\nmoc4-2.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0123769
Feature type
allele
Associated gene
Dmel\nmo
Associated Insertion(s)
Carried in Construct
P{UAS-nmo.c4-2}
Key Links
Transgenic product class
frameshift_elongation
(Garelli, 2010.8.20)
Mutagen
Nature of the Allele
Transgenic product class
frameshift_elongation
(Garelli, 2010.8.20)
Mutagen
in vitro construct
(Verheyen et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-nmo.c4-2}
Cytology
Description

Analysis suggests that the 477 amino acid protein isoform encoded by the P{UAS-nmo.c4-2} construct does not correspond to an isoform actually expressed in flies and is probably the result of an error at the moment of cDNA synthesis. The protein (see GenBank accession U12009.1) is not predicted in the D. melanogaster genome, or in any of the other sequenced Drosophila species and there is no other EST, besides the clone that the construct was made from, that encodes this isoform. The sequence of the clone used to generate the construct is almost the same as the mRNA that encodes the shortest nmo isoform, but it includes an extra base at the end of the coding region that produces a frameshift. As a result of this shift, the protein encoded by P{UAS-nmo.c4-2} has 63 more amino acids than the nmo short isoform. This change does not alter the kinase domain of nmo.

(Garelli, 2010.8.20)

UASt regulatory sequences drive expression of a full length cDNA corresponding to nmo isoform c4-2.

(Verheyen et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nmo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Although the protein isoform encoded by the P{UAS-nmo.c4-2} construct does not correspond to an isoform actually expressed in flies, the protein produced is almost the same as the shortest nmo isoform; a frameshift results in P{UAS-nmo.c4-2} encoding a protein that has 63 more amino acids than the nmo short isoform. This change does not alter the kinase domain, which might be the reason that overexpression P{UAS-nmo.c4-2} under the control of Scer\GAL469B results in a similar phenotype to overexpression of P{UAS-nmo.c5-1}.

      (Garelli, 2010.8.20)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nmoc4-2.Scer\UAS
      nmoc4-2.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)