FlyBase Allele Report: Dmel\nmo[adk2]

General Information

Symbol

Dmel\nmo^adk2

Species

D. melanogaster

Name

FlyBase ID

FBal0057940

Feature type

allele

Associated gene

Dmel\nmo

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Verheyen et al., 1996)

Progenitor genotype

Cytology

Description

Mutation resulting in alterations in the pattern of transcripts produced (truncated transcripts are produced in pupae).

(Verheyen et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Ju et al., 2013)

ameliorates Kennedy's disease

modeled by Hsap\AR^61Q.UAS

(Todd et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | recessive

(Verheyen et al., 2001)

decreased cell size (with nmo^DB24)

(Zeng et al., 2007)

partially lethal - majority die | recessive

(Verheyen et al., 1996)

visible

(Zeng et al., 2007)

visible (with nmo^DB24)

(Zeng et al., 2007)

visible (with nmo^P1)

(Verheyen et al., 2001)

visible | recessive

(Verheyen et al., 2001)

Phenotype Manifest In

adult head bristle

(Verheyen et al., 2001)

crossvein | ectopic

(Verheyen et al., 1996)

eye

(Mirkovic et al., 2002, Verheyen et al., 1996)

eye (with nmo^P1)

(Verheyen et al., 2001)

mesothoracic tergum & macrochaeta

(Verheyen et al., 2001)

ommatidium

(Mirkovic et al., 2002, Verheyen et al., 1996)

ommatidium (with nmo^P1)

(Verheyen et al., 2001)

secondary pigment cell | ectopic

(Mirkovic et al., 2002)

tertiary pigment cell | ectopic

(Mirkovic et al., 2002)

wing

(Verheyen et al., 2001, Verheyen et al., 1996)

wing blade

(Zeng et al., 2007)

wing blade (with nmo^DB24)

(Zeng et al., 2007)

wing vein | ectopic

(Zeng et al., 2007, Verheyen et al., 2001)

wing vein | ectopic (with nmo^DB24)

(Lee et al., 2009)

Detailed Description

Statement

Reference

Ectopic wing veins are observed in nmo^DB24/nmo^adk2 mutant animals.

(Lee et al., 2009)

Homozygotes have a broader wing blade than normal and have ectopic veins emanating from the posterior crossvein, posterior to L5 and between L2 and L3. The positions of veins L2 and L5 are shifted from the central anterior-posterior boundary towards the margins compared to wild type.

nmo^adk2/nmo^DB24 flies have more cells per given area of the wing blade compared to controls, suggesting that the mutant cells are slightly smaller than wild-type cells. In addition, the wings are consistently larger than wild-type wings.

(Zeng et al., 2007)

Post death stage homozygous nmo^adk2 pupal retinae have an increased average density of secondary/tertiary pigment cells compared to wild-type. Unlike wild-type, the local density of these cells is variable: dependent on the specific abberations in ommatidial shape and orientation in the region in question. In some regions the ommatidia are square-like and have approximately wild-type numbers of secondary/tertiary cells. In other regions of the same retina, ommatidia retain a hexagonal-like shape but have increased numbers of secondary/tertiary pigment cells. nmo^adk2 pupal retinae have a decreased level of cell death compared to wild-type at multiple developmental time points.

(Mirkovic et al., 2002)

nmo^P1/nmo^adk2 flies have a smaller eye than normal in which the ommatidia are square instead of the normal hexagonal shape and are generally flanked by 4 interommatidial bristles (instead of the usual 3 bristles at alternating corners). Mutant flies have a shorter, broader wing than normal. Excess wing vein tissue originating from the posterior crossvein and extending distally between veins L4 and L5 is seen (this phenotype is fully penetrant). Additional vein material can also form parallel to vein L2 and posterior to vein L5. The wings are held away from the body at a 45^o angle. Polarity defects are seen in those regions in the wing where ectopic veins are seen. Homozygotes show a mild loss of bristles on the head and notum (having an average of 37.0 +/- 1.60 bristles compared to the wild-type number of 40) and occasionally have bent or duplicated bristles.

(Verheyen et al., 2001)

Semi-lethal, rare homozygous flies display rough, small eyes and several wing phenotypes. Wings are rounder and shorter and held out at an angle from the body, and also display ectopic cross vein material.

(Verheyen et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nmo^adk2 has visible phenotype, enhanceable by px¹

(Verheyen et al., 2001)

nmo^adk2 has visible phenotype, enhanceable by dpp^s1

(Verheyen et al., 2001)

nmo^adk2 has visible phenotype, enhanceable by net¹

(Verheyen et al., 2001)

Suppressed by

Statement

Reference

nmo^adk2/nmo^adk1 has visible phenotype, suppressible by Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa

(Verheyen et al., 2001)

nmo^adk2 has visible | recessive phenotype, suppressible by H¹

(Verheyen et al., 2001)

Enhancer of

Statement

Reference

nmo^adk2/nmo^adk2 is an enhancer of visible | dominant phenotype of Delta^9M

(Verheyen et al., 2001)

nmo^adk2 is an enhancer of visible phenotype of px¹

(Verheyen et al., 2001)

nmo^adk2/nmo^adk1 is an enhancer of visible | dominant phenotype of H¹

(Verheyen et al., 2001)

nmo^adk2 is an enhancer of visible phenotype of net¹

(Verheyen et al., 2001)

Suppressor of

Statement

Reference

nmo^adk2/nmo[+] is a suppressor of visible phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Ju et al., 2013)

nmo^adk2/nmo[+] is a suppressor of abnormal eye color phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Ju et al., 2013)

nmo^adk2/nmo[+] is a suppressor of visible phenotype of rpr^GMR.PH

(Mirkovic et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

nmo^adk2 has wing vein phenotype, enhanceable by px¹

(Verheyen et al., 2001)

nmo^adk2 has wing vein | ectopic phenotype, enhanceable by dpp^s1

(Verheyen et al., 2001)

nmo^adk2 has wing vein phenotype, enhanceable by net¹

(Verheyen et al., 2001)

Suppressed by

Statement

Reference

nmo^adk2/nmo^adk1 has wing vein | ectopic phenotype, suppressible by Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa

(Verheyen et al., 2001)

nmo^adk2 has wing vein | ectopic phenotype, suppressible by H¹

(Verheyen et al., 2001)

Enhancer of

Statement

Reference

nmo^adk2/nmo^adk2 is an enhancer of wing vein phenotype of Delta^9M

(Verheyen et al., 2001)

nmo^adk2 is an enhancer of wing vein phenotype of px¹

(Verheyen et al., 2001)

nmo^adk2/nmo^adk1 is an enhancer of adult head bristle phenotype of H¹

(Verheyen et al., 2001)

nmo^adk2/nmo^adk1 is an enhancer of mesothoracic tergum & macrochaeta phenotype of H¹

(Verheyen et al., 2001)

nmo^adk2 is an enhancer of wing vein phenotype of net¹

(Verheyen et al., 2001)

Suppressor of

Statement

Reference

nmo^adk2/nmo[+] is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Ju et al., 2013)

nmo^adk2/nmo[+] is a suppressor of eye phenotype of rpr^GMR.PH

(Mirkovic et al., 2002)

nmo^adk2 is a suppressor of phenotype of N^nucl.sev

(Verheyen et al., 1996)

Other

Statement

Reference

Df(2L)flp147E/+, nmo^adk2 has wing phenotype

(Mirkovic et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for nmo^adk2 partially suppresses the small eye size of rpr^GMR.PH animals. Wings from flies homozygous for nmo^adk2 and heterozygous for Df(2L)flp147E are fragile and display apparent defects in epithelial integrity not normally observed in nmo^adk2 mutants.

(Mirkovic et al., 2002)

The mild wing vein thickening phenotype seen in Dl^9M/+ flies is synergistically enhanced by homozygosity for nmo^adk2. The ectopic wing veins seen in nmo^adk2 flies are completely suppressed by H¹. The loss of bristles on the head and notum seen in H¹/+ flies is dramatically enhanced by nmo^adk1/nmo^adk2. Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B completely suppresses the extra wing vein phenotype of nmo^adk1/nmo^adk2 flies. px¹ nmo^adk2 double mutant flies show a synergistic interaction with respect to the extra wing vein phenotype of each single mutant. net¹ nmo^adk2 double mutant flies show a strong synergistic interaction with respect to the extra wing vein phenotype of each single mutant. dpp^s1 nmo^adk2 flies show an enhancement of the nmo^adk2 ectopic wing vein phenotype, especially in the anterior of the wing blade near vein L2.

(Verheyen et al., 2001)

Second site dominant suppressor of N^nucl.sev that reduces the external roughness of the eye, in addition to causing the overall eye shape to become both rounder and larger, and less refractile.

(Verheyen et al., 1996)

Xenogenetic Interactions

Statement

Reference

One copy of nmo^adk2 strongly suppresses the retinal depigmentation and ommatidial disorganisation phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed in the eye under the control of Scer\GAL4^GMR.PU.

(Ju et al., 2013)

Complementation and Rescue Data

Rescued by

nmo^adk2/nmo^adk1 is rescued by nmo^c5-1.UAS/Scer\GAL4^bs-1348

(Verheyen et al., 2001)

Comments

Expression of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^bs-1348 rescues the extra wing vein phenotype seen in nmo^adk1/nmo^adk2 flies.

(Verheyen et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

adk^sDU2

nmo^adk2

(Todd et al., 2015, Ju et al., 2013, Lee et al., 2009, Zeng et al., 2007)

nmo^sDU2

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close