FlyBase Insertion Report: Dmel\P{PZ}CCT8[03996]

General Information

Symbol

Dmel\P{PZ}CCT8⁰³⁹⁹⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0005256

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03996

Affected gene(s)

CCT8, Ecol\lacZ

Viability / fertility

Associated allele(s)

CCT8⁰³⁹⁹⁶, Ecol\lacZ⁰³⁹⁹⁶

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 44F5 )

Sequence Location

2R:8,907,003..8,907,003 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:8,907,003..8,907,003 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

44F5 (inferred by FlyBase from sequence location)

Cytological location (reported)

44F3-44F4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025620

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

CCT8

(Kahsai et al., 2016, Misra, 2000.8.25, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

CCT8⁰³⁹⁹⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ⁰³⁹⁹⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult optic lobe | restricted

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03996

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11361

cn¹ P{PZ}CCT8⁰³⁹⁹⁶/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion.

(Kahsai et al., 2016)

Location 2R:4418815-4418816 determined by FlyBase alignment of dbGSS accession AQ025620 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

The P{PZ}l(2)03996⁰³⁹⁹⁶ insertion maps 7bp upstream of the 5\' end of the CT8297 transcript of CG8258.

(Misra, 2000.8.25)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1361

(Meister and Braun, 1995.10)

P{PZ}CCT8⁰³⁹⁹⁶

P{PZ}l(2)03996⁰³⁹⁹⁶

(Kahsai et al., 2016, FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)03996⁰³⁹⁹⁶

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000341

References (12)

Report Sections

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