FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\nrv2
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General Information
Symbol
Dmel\nrv2
Species
D. melanogaster
Name
nervana 2
Annotation Symbol
CG9261
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015777
Gene Model Status
Current
Stock Availability
33 publicly available
Gene Summary
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. (UniProt, Q24048)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

l(2)k13315, Nervana, Nrv2.2, Na+/K+ ATPase, l(2)k04223

Key Links
Genomic Location
Cytogenetic map
27B1-27B2
Sequence location
Recombination map
2-22
RefSeq locus
NT_033779 REGION:6789770..6798862
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (20 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables ATPase activator activity
inferred from direct assay
(Sun et al., 1998)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables ATPase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
Biological Process (15 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in cell adhesion involved in heart morphogenesis
inferred from mutant phenotype
(Yi et al., 2008)
involved_in establishment of glial blood-brain barrier
inferred from mutant phenotype
(Stork et al., 2008, Schwabe et al., 2005)
involved_in monoatomic cation transport
inferred from direct assay
(Sun et al., 1998)
involved_in open tracheal system development
inferred from mutant phenotype
(Beitel and Krasnow, 2000)
involved_in regulation of tube diameter, open tracheal system
inferred from mutant phenotype
(Paul et al., 2003)
involved_in regulation of tube length, open tracheal system
inferred from mutant phenotype
(Paul et al., 2003)
involved_in regulation of tube size, open tracheal system
inferred from mutant phenotype
(Beitel and Krasnow, 2000)
involved_in sensory perception of sound
inferred from mutant phenotype
(Roy et al., 2013)
involved_in septate junction assembly
inferred from mutant phenotype
(Paul et al., 2003)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in intracellular potassium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
involved_in intracellular sodium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
involved_in potassium ion import across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
involved_in potassium ion transport
inferred from electronic annotation with InterPro:IPR000402
(InterPro Project Members, 2004-)
involved_in sodium ion export across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
involved_in sodium ion transport
inferred from electronic annotation with InterPro:IPR000402
(InterPro Project Members, 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lowe et al., 2014)
located_in plasma membrane
inferred from direct assay
(Yi et al., 2008)
inferred from high throughput direct assay
(Tan et al., 2009)
located_in septate junction
inferred from direct assay
(Paul et al., 2007, Genova and Fehon, 2003)
part_of sodium:potassium-exchanging ATPase complex
inferred from direct assay
(Sun et al., 1998)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of sodium:potassium-exchanging ATPase complex
inferred from electronic annotation with InterPro:IPR000402
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the X(+)/potassium ATPases subunit beta family. (Q24048)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
NA+/K+ TRANSPORTING P-ATPASE - BETA SUBUNITS -
Na+/K+ transporting ATPase β-subunit is a type II membrane protein required for the structural and functional maturation of the α-subunit and membrane targeting. (Adapted from FBrf0210681).
Protein Function (UniProtKB)
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
(UniProt, Q24048)
Summary (Interactive Fly)

β subunit of Na,K-ATPase, plays an ion-pump-independent role in junction formation and tracheal morphogenesis - important for maintaining cellular membrane potential in muscles

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\nrv2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q24048)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
nrv2-RA
FBtr0079382
NM_057821
1980
322
nrv2-RC
FBtr0079381
NM_057820
2668
323
nrv2-RD
FBtr0079383
NM_164711
2156
322
nrv2-RE
FBtr0100381
NM_001014476
1949
322
nrv2-RF
FBtr0100382
NM_001014475
2143
323
nrv2-RG
FBtr0332369
NM_001273235
2635
323
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
nrv2-PA
FBpp0079010
37.0
322
8.03
Q1RL12
NP_477169
AAF52438
nrv2-PC
FBpp0079009
37.3
323
7.39
Q24048
NP_477168
AAN10600
nrv2-PD
FBpp0079011
37.0
322
8.03
Q1RL12
NP_723216
AAF52439
nrv2-PE
FBpp0099793
37.0
322
8.03
Q1RL12
NP_001014476
AAX52660
nrv2-PF
FBpp0099794
37.3
323
7.39
Q24048
NP_001014475
AAX52659
nrv2-PG
FBpp0304645
37.3
323
7.39
Q24048
NP_001260164
AGB92700
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

322 aa isoforms: nrv2-PA, nrv2-PD, nrv2-PE
323 aa isoforms: nrv2-PC, nrv2-PF, nrv2-PG
Additional Polypeptide Data and Comments
Reported size (kDa)

323, 322 (aa); 42 (kD observed)

(Sun and Salvaterra, 1995)
Comments

Nervana (nerve antigen) protein is an anti-HRP

reactive ~42K glycoprotein that is likely to be the major contributor to

neuronal-specific anti-HRP staining. Protein was isolated from adult heads

and monoclonal antibodies were made. Staining with the monoclonal

antibodies reveals two or three products between 38K and 42K. It is

unclear if the properties described apply to nrv1 protein, nrv2

protein, or both.

(Sun et al., 1998)

nrv1 protein and/or nrv2 protein can be copurified with the α subunit of Drosophila Na+,K+-ATPase from extracts of Drosophila heads.

(Sun and Salvaterra, 1995)

Nrv 2.1

(FlyBase, 1992-)
External Data
Subunit Structure (UniProtKB)

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.

(UniProt, Q24048)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nrv2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.66

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain surface glial cell
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval lateral VNC surface glial cell
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
medial-most cell body glial cell
(Kearney et al., 2004)
RNase protection, primer extension, SI map
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
abdomen
(Sun et al., 1998)
(Sun et al., 1998)
adult head
(Sun et al., 1998)
adult abdomen
(Sun et al., 1998)
Additional Descriptive Data

The 2.1kb isoform of nrv2 (Nrv2.2) is the major form expressed in fly heads. It is expressed at low levels in the abdomen.

(Sun et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
dissected tissue
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Sun et al., 1998)
adult thorax
(Sun et al., 1998)
adult optic lobe
(Sun et al., 1998)
adult ventral nerve cord
(Sun et al., 1998)
adult brain
(Sun et al., 1998)
leg
(Sun et al., 1998)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
larval longitudinal connective
(Sun and Salvaterra, 1995)
adult stage
scolopale cell of Johnston organ
(Roy et al., 2013)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
central nervous system
(Sun and Salvaterra, 1995)
symmetrical commissure
(Sun and Salvaterra, 1995)
embryonic/larval posterior spiracle
(Sun and Salvaterra, 1995)
presumptive embryonic/larval peripheral nervous system
(Sun and Salvaterra, 1995)
embryonic/larval garland cell
(Sun and Salvaterra, 1995)
embryonic stage -- adult stage
head
(Sun and Salvaterra, 1995)
adult stage
adult thorax
(Baumann et al., 2010, Sun and Salvaterra, 1995)
adult head
(Baumann et al., 2010, Sun and Salvaterra, 1995)
adult abdomen
(Sun and Salvaterra, 1995)
Additional Descriptive Data

nrv2 is detected in adult head and weakly in thorax by western blot.

(Baumann et al., 2010)

nrv2 is nervous system-specific. On western blots of dissected tissues, it is detected in adult heads and thorax, presumably in nervous system components. In adult tissue sections, it is detected predominantly in the nervous system, including the optic lobe, brain, thoracic ganglia, and axonal pathways in the leg.

(Sun et al., 1998)

Nervana protein is detected in adult heads, thoraces and abdomens but is significantly enriched in heads on western blots. On developmental westerns, it is first observed in 6hr embryos and then throughout development. The levels of the different mobility components change through development. The 42K component is not observed until 12-24hr and is not prominent until the adult stage. Nervana protein is observed in the CNS and PNS by immunolocalization in a pattern indistinguishable from the anti-HRP pattern. Staining is also observed in the garland cells and the posterior spiracles.

(Sun and Salvaterra, 1995)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lowe et al., 2014)
located_in plasma membrane
inferred from direct assay
(Yi et al., 2008)
inferred from high throughput direct assay
(Tan et al., 2009)
located_in septate junction
inferred from direct assay
(Paul et al., 2007, Genova and Fehon, 2003)
part_of sodium:potassium-exchanging ATPase complex
inferred from direct assay
(Sun et al., 1998)
Expression Deduced from Reporters
Reporter: P{nrv2-GAL4.S}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
nervous system
(Sun et al., 1999)
neuron
(Sun et al., 1999)
embryonic stage 16
dorsal channel glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
longitudinal glial cell

Comment: lateral posterior in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
medial intersegmental nerve root glial cell
(Beckervordersandforth et al., 2008)
lateral segmental nerve root glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
medial-most cell body glial cell
(Beckervordersandforth et al., 2008)
medial cell body glial cell
(Beckervordersandforth et al., 2008)
lateral cell body glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
larval stage
larval glial cell | subset of embryonic/larval optic lobe
(Edwards et al., 2012)
larval stage -- adult stage
neuron | subset & glial cell | subset of central nervous system
(Sun et al., 2001)
third instar larval stage
embryonic/larval wrapping glial cell
(Coutinho-Budd et al., 2017, Schmidt et al., 2012)
embryonic/larval cell body glial cell
(Coutinho-Budd et al., 2017, Spéder and Brand, 2014)
astrocyte-like glial cell
(Coutinho-Budd et al., 2017)
embryonic/larval ensheathing glial cell
(Coutinho-Budd et al., 2017)
pupal stage
glial cell | subset of adult optic lobe
(Edwards et al., 2012)
pupal stage P7
developing satellite glial cell | subset
(Edwards et al., 2012)
pharate adult stage -- adult stage
adult distal lamina satellite glial cell
(Edwards et al., 2012)
adult stage
adult optic lobe

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
lamina

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
medulla

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
glial cell | subset of adult optic lobe
(Shimozono et al., 2019, Górska-Andrzejak et al., 2009)
adult lamina satellite glial cell
(Górska-Andrzejak et al., 2009)
adult inner optic chiasma giant glial cell
(Górska-Andrzejak et al., 2009)
adult outer optic chiasma giant glial cell
(Górska-Andrzejak et al., 2009)
adult medulla neuropil associated glial cell
(Górska-Andrzejak et al., 2009)
adult cerebrum
(Górska-Andrzejak et al., 2009)
glial cell of antennal lobe
(Oland et al., 2008)
Reporter: P{nrv2-GAL4.U}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval wrapping glial cell
(Otto et al., 2018)
embryonic/larval cell body glial cell
(Otto et al., 2018)
embryonic/larval ensheathing glial cell
(Otto et al., 2018)
embryonic/larval astrocyte-like glial cell
(Otto et al., 2018)
Reporter: P{nrv2-lacZ.7.7}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 17
larval central nervous system
(Xu et al., 1999)
larval peripheral nervous system
(Xu et al., 1999)
adult stage
adult brain
(Xu et al., 1999)
adult ventral nerve cord
(Xu et al., 1999)
supraesophageal ganglion
(Xu et al., 1999)
adult gnathal ganglion
(Xu et al., 1999)
adult optic lobe
(Xu et al., 1999)
adult optic lobe
(Xu et al., 1999)
Reporter: P{nrv2-VP16.AD}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 12
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
embryonic stage 15
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
first instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
second instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
third instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
adult stage
adult brain cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
Reporter: P{PTT-GA}nrv2ZCL1649
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
follicle cell
(Cooley et al., 2003.5)
oocyte
(Cooley et al., 2003.5)
nurse cell
(Cooley et al., 2003.5)
Reporter: P{PTT-un}Nrv2PTT
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16
ectoderm | restricted
(Edenfeld et al., 2006)
larval nervous system | restricted
(Edenfeld et al., 2006)
Reporter: P{PTT-un}nrv2ZCL2903
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
cyst cell of testis
(Cash and Andrews, 2012)
adult stage | female
germarium | restricted
(Cash and Andrews, 2012)
Reporter: P{unk}nrv2173
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
glial cell | subset of larval peripheral nervous system
(Stork et al., 2008)
Reporter: PBac{681.P.FSVS-1}nrv2CPTI001455
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
Reporter: PBac{CPTI-un}nrv2CPTI001459
Stage
Tissue/Position (including subcellular localization)
Reference
first instar larval stage
motor neuron
• axon

Comment: axons

(Lowe et al., 2014)
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nrv2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of nrv2
Transgenic constructs containing regulatory region of nrv2
Aberrations (Deficiencies and Duplications) ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4elav.PU
lethal, with Scer\GAL4repo.PU
lethal (with Df(2L)ED6569)
lethal | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal auditory perception, with Scer\GAL4nompA.cK
abnormal circadian rhythm | adult stage, with Scer\GAL4repo
abnormal neuroanatomy
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
adherens junction | P-stage | somatic clone
cell-cell junction | P-stage | somatic clone
embryonic/larval tracheal dorsal trunk
embryonic/larval tracheal transverse connective
embryonic ganglionic branch & embryonic tracheole
embryonic somatic muscle cell
epithelial cell | P-stage | somatic clone
epithelial cell | somatic clone
ganglionic tracheal branch primordium
Johnston organ, with Scer\GAL4nompA.cK
larval tracheal system
mesonotum | P-stage | somatic clone
midbody | somatic clone
presumptive embryonic salivary gland
septate junction
septate junction & embryonic tracheal system
septate junction | somatic clone
tracheal branch primordium
tracheal dorsal trunk primordium
trichogen cell, with Scer\GAL4pnr-MD237
vesicle | P-stage | increased number | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\ATP1B1
11 of 14
Yes
No
1  
Hsap\ATP1B2
11 of 14
Yes
No
Hsap\ATP1B3
11 of 14
Yes
Yes
1  
Hsap\ATP1B4
11 of 14
Yes
Yes
Hsap\ATP4B
8 of 14
No
Yes
Hsap\SHBG
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Atp1b1
12 of 14
Yes
No
Rnor\Atp1b2
11 of 14
No
No
Rnor\Atp1b4
11 of 14
No
Yes
Rnor\Atp1b3
9 of 14
No
Yes
Rnor\Atp4b
9 of 14
No
Yes
Rnor\Shbg
1 of 14
No
Yes
Mus musculus (laboratory mouse) (6)
Mmus\Atp1b1
11 of 14
Yes
No
Mmus\Atp1b2
11 of 14
Yes
No
Mmus\Atp1b4
11 of 14
Yes
Yes
Mmus\Atp1b3
9 of 14
No
Yes
Mmus\Atp4b
9 of 14
No
Yes
Mmus\Shbg
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (8)
Xtro\atp1b3
10 of 13
Yes
Yes
Xtro\atp4b
8 of 13
No
Yes
Xtro\atp1b1
6 of 13
No
No
Xtro\atp1b2
6 of 13
No
No
Xtro\atp1b4
6 of 13
No
Yes
Xtro\acox1
1 of 13
No
No
Xtro\LOC100485787
1 of 13
No
Yes
Xtro\slc25a34
1 of 13
No
No
Danio rerio (Zebrafish) (8)
Drer\atp1b1a
12 of 14
Yes
No
Drer\atp1b1b
12 of 14
Yes
No
Drer\atp1b2b
11 of 14
No
No
Drer\atp1b3a
11 of 14
No
Yes
Drer\atp1b3b
11 of 14
No
Yes
Drer\atp1b4
11 of 14
No
Yes
Drer\atp1b2a
10 of 14
No
No
Drer\shbg
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\nkb-1
14 of 14
Yes
Yes
Cele\nkb-3
14 of 14
Yes
Yes
Cele\nkb-2
5 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (3)
Agam\AgaP_AGAP007791
11 of 12
Yes
Yes
Agam\AgaP_AGAP009595
6 of 12
No
No
Agam\AgaP_AGAP007790
5 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:nrv2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (6)
nrv1
11 of 13
nrv3
11 of 13
CG11703
6 of 13
CG5250
5 of 13
CG33310
5 of 13
GABA-B-R1
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    nrv2HMS01637
    model of  epilepsy
    CEA
    (Hope et al., 2020)
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    ATP1B1; ATPase Na+/K+ transporting subunit beta 1
    model of  essential hypertension
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    nrv2k13315
    exacerbates  Huntington's disease
    modeled by Hsap\HTT128Q.1-336.UAS
    (Shirasaki et al., 2012)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ATP1B1; ATPase Na+/K+ transporting subunit beta 1
    11 of 14
    182330
     
    ATP1B2; ATPase Na+/K+ transporting subunit beta 2
    11 of 14
    182331
        ATP1B3; ATPase Na+/K+ transporting subunit beta 3
        11 of 14
        601867
             
            ATP1B4; ATPase Na+/K+ transporting family member beta 4
            11 of 14
            301073
                ATP4B; ATPase H+/K+ transporting subunit beta
                8 of 14
                137217
                    Functional Complementation Data
                    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                    Interactions
                    Summary of Physical Interactions
                    Summary of Genetic Interactions
                    Interaction Browsers
                    View in FlyBase Interactions Browser   View in MIST tool (external link)
                    Please look at the allele data for full details of the genetic interactions
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    nrv2
                    suppressible
                    nrv2::nrv3
                    (Paul et al., 2007)
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    External Data
                    Subunit Structure (UniProtKB)
                    The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
                    (UniProt, Q24048 )
                    Linkouts
                    DroID - A comprehensive database of gene and protein interactions.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Pathways
                    Signaling Pathways (FlyBase)
                    Metabolic Pathways
                    FlyBase
                    External Links
                    External Data
                    Linkouts
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    Class of Gene
                    Genomic Location and Detailed Mapping Data
                    Chromosome (arm)
                    2L
                    Recombination map
                    2-22
                    Cytogenetic map
                    27B1-27B2
                    Sequence location
                    FlyBase Computed Cytological Location
                    Cytogenetic map
                    Evidence for location
                    27B1-27B2
                    Experimentally Determined Cytological Location
                    Cytogenetic map
                    Notes
                    References
                    26F7-27A1
                    (Oh et al., 2003)
                    27B1-27B2
                    (determined by in situ hybridisation)
                    (Beitel and Krasnow, 2000, Spradling et al., 1999, Williamson et al., 1998)
                    27A-27B
                    (determined by in situ hybridisation)
                    (Salveterra, 1999.1.20)
                    92C-92D
                    (determined by in situ hybridisation)
                    (Sun and Salvaterra, 1995)
                    27B1-27B2
                    (BDGP Project Members, 1994-1999)
                    Experimentally Determined Recombination Data
                    Location

                    2-22

                    (FlyBase, 2016)

                    2-21.6

                    (Comeron et al., 2012)

                    2-

                    Left of (cM)
                    Right of (cM)
                    Notes
                    Stocks and Reagents
                    Stocks (33)
                    Genomic Clones (21)
                    cDNA Clones (150)
                     

                    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                    cDNA clones, fully sequenced
                    BDGP DGC clones
                    Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    Antibody Information
                    Laboratory Generated Antibodies
                     

                    polyclonal

                    (Sun et al., 1998)

                    monoclonal

                    (Sun and Salvaterra, 1995)
                    Commercially Available Antibodies
                     
                    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                    Cell Line Information
                    Publicly Available Cell Lines
                     
                      Other Stable Cell Lines
                       
                        Other Comments

                        1 allele of nrv2 recovered in a P-insertion screen.

                        (Oh et al., 2003)

                        Mutant embryos have lengthened primary tracheal tubes with expanded diameters, and defective septate junctions.

                        (Paul et al., 2003)

                        Area matching Drosophila Neuron surface antigen 2 gene, Acc. No. U22439.

                        (Davies, 2001.3.15)

                        Mutation in nrv2 alters tracheal tube size in embryos without affecting other aspects of tracheal development including primary branch budding and outgrowth or branch fusion.

                        (Beitel and Krasnow, 2000)

                        The product(s) of the Nervana gene(s) were recognised by their epitope(s) which reacts with anti-Horse Radish Peroxidase antibodies. These antibodies label neurons in Drosophila. The nac gene product may be required for the glycosylation of these proteins.

                        (Sun and Salvaterra, 1995)

                        Southern blot of cRNA probes hybridised to genomic DNA indicates nrv1 and nrv2 are derived from different genes.

                        (Sun and Salvaterra, 1995)

                        nrv1 and nrv2 are neuron-specific forms of the β subunit of Na+,K+-ATPase.

                        (Sun and Salvaterra, 1995)
                        Relationship to Other Genes
                        Source for database merge of

                        Source for merge of: nrv2 l(2)SH0329

                        (FlyBase, 1992-)

                        Source for merge of: nrv2 NEST:bs13c10

                        (Paul et al., 2003, FlyBase, 1996-)

                        Source for merge of: nrc2 ecta

                        (Paul et al., 2003)
                        Additional comments

                        Source for merge of nrv2 NEST:bs13c10 was sequence comparison ( date:031021 ).

                        (FlyBase, 1996-)
                        Nomenclature History
                        Source for database identify of

                        Source for identity of: Nrv2 CG9261

                        (Wortman, 1999.11)

                        Source for identity of: ecta l(2)k04223

                        (Beitel and Krasnow, 2000)
                        Nomenclature comments
                        Etymology
                        Synonyms and Secondary IDs (29)
                        Reported As
                        Symbol Synonym
                        42kDa
                        (Pahud et al., 1998)
                        CG9261
                        (National Institute of Genetics Fly Stocks, 2022-, Meadows, 2017.1.11, Çiçek et al., 2016, Jaspers et al., 2012, Cammarato et al., 2011, Ling and Salvaterra, 2011, Ni et al., 2011.7.19, Khanna et al., 2010, Perkins et al., 2009, Schiess et al., 2009, Quinones-Coello, 2007, Rendic et al., 2006)
                        NEST:bs13c10
                        Na,K-ATPase
                        (Okamura et al., 2001)
                        Na+/K+ ATPase
                        (Chen and Haddad, 2004)
                        Na+/K+-ATPase
                        (Linton and O'Donnell, 1999)
                        Na+/K+ATPase
                        (Chen et al., 2002)
                        NaK ATPase
                        (Wu and Beitel, 2003)
                        Nrv
                        Nrv2
                        (Banach-Latapy et al., 2023, Petsakou and Perrimon, 2023, Lim et al., 2019, Spéder and Brand, 2018, Lowe et al., 2014, Chen and Zhang, 2013, Górska-Andrzejak, 2013, Górska-Andrzejak et al., 2009, Larsen et al., 2009, Fung et al., 2008, Oland et al., 2008, Paul et al., 2007, Altenhein et al., 2006, Wang et al., 2004, Cooley et al., 2003.5, Bellen, 2000.8.28, Prokop, 1999, Emery et al., 1998, Sun et al., 1998)
                        Nrv2.2
                        (Dalla et al., 2017, Dalla and Dobler, 2016, Gorokhova et al., 2007)
                        ecta
                        l(2)SH0329
                        l(2)SH2 0329
                        (Oh et al., 2002.3.22)
                        l(2)k04223
                        (Spradling et al., 1999)
                        l(2)k13315
                        (Kaufmann et al., 2002, Spradling et al., 1999)
                        nrv2
                        (Mira-Osuna et al., 2025, Overgaard et al., 2025, Isaacson et al., 2024, Lee et al., 2024, Titlow et al., 2023, Chen et al., 2022, Hodge et al., 2022, Jia et al., 2022, Liu et al., 2022, Rouka et al., 2021, Yazar et al., 2021, Hope et al., 2020, Miller and Posakony, 2020, Ariss et al., 2018, Carvalho et al., 2018, Croset et al., 2018, Daniel et al., 2018, Charlton-Perkins et al., 2017, Tempesta et al., 2017, Transgenic RNAi Project members, 2017-, Altenhein et al., 2016, Sarov et al., 2016, Gene Disruption Project members, 2015-, Lin et al., 2015, DeSalvo et al., 2014, Fedotov et al., 2014, Hall et al., 2014, Kerr et al., 2014, Luan et al., 2014, Ma and Weake, 2014, Mukherjee et al., 2014, Robbins et al., 2014, Roy et al., 2013, von Hilchen et al., 2013, Cash and Andrews, 2012, Japanese National Institute of Genetics, 2012.5.21, Nelson et al., 2012, Shirasaki et al., 2012, Friedman et al., 2011, Guan et al., 2011, Ling and Salvaterra, 2011, Xie and Auld, 2011, Baumann et al., 2010, Nilton et al., 2010, Spindler et al., 2009, Stork et al., 2009, Tan et al., 2009, Quijano et al., 2008, Stork et al., 2008, Yi et al., 2008, Baer et al., 2007, Dietzl et al., 2007, Li et al., 2007, Paul et al., 2007, Quinones-Coello, 2007, Wu et al., 2007, Tolhuis et al., 2006, Younossi-Hartenstein et al., 2006, Schwabe et al., 2005)
                        nrv2.2
                        (Rendic et al., 2006)
                        Name Synonyms
                        Na+/K+ ATPase
                        (Nilton et al., 2010)
                        Na,K-ATPase β-subunit
                        (Dalla and Dobler, 2016)
                        Na+/K+ ATPase
                        (Ling and Salvaterra, 2011)
                        Nervana
                        (Beramendi et al., 2007, Rendic et al., 2006, Genova and Fehon, 2001)
                        Nervana 2
                        (Mira-Osuna and Borgne, 2024, Rice et al., 2021, Lowe et al., 2014, Chen and Zhang, 2013, Górska-Andrzejak, 2013, Guan et al., 2011, Xie and Auld, 2011, Khanna et al., 2010, Górska-Andrzejak et al., 2009, Tan et al., 2009)
                        Nervana2
                        (Rodrigues et al., 2011, Oland et al., 2008, Roy et al., 2007)
                        Nirvana
                        ectatic
                        (Beitel and Krasnow, 2000)
                        nervana
                        (Sun and Salvaterra, 1995)
                        nervana 2
                        (Saito et al., 2018, Cash and Andrews, 2012, Jaspers et al., 2012, Cammarato et al., 2011, Nilton et al., 2010, Baer et al., 2007)
                        nervana2
                        (Stork et al., 2008, Yi et al., 2008, Nair et al., 2007, Morin et al., 2001)
                        nirvana2
                        (Spindler et al., 2009)
                        Secondary FlyBase IDs
                        • FBgn0014867
                        • FBgn0021885
                        • FBgn0022206
                        • FBgn0045319
                        • FBgn0065838
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        Study result (0)
                        Result
                        Result Type
                        Title
                        External Crossreferences and Linkouts ( 68 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                        Other crossreferences
                        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                        FlyMine - An integrated database for Drosophila genomics
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        MARRVEL_MODEL - MARRVEL (model organism gene)
                        Linkouts
                        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                        DroID - A comprehensive database of gene and protein interactions.
                        DRSC - Results frm RNAi screens
                        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                        Flygut - An atlas of the Drosophila adult midgut
                        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                        References (210)