FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nrv2nwu3
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General Information
Symbol
Dmel\nrv2nwu3
Species
D. melanogaster
Name
FlyBase ID
FBal0151679
Feature type
allele
Associated gene
Dmel\nrv2
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Paul et al., 2003)
Mutagen
P-element activity
(Paul et al., 2003)
Progenitor genotype
nrv2k04223
(Paul et al., 2003)
Cytology
Description

Imprecise excision of P{lacW}nrv2k04223 has deleted the first two and most of the third common exons of nrv2. The genomic sequence across the deletion breakpoints is AAGGCCCTCGCTATAACCAAGGAGAATAAC.

(Paul et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      embryonic ganglionic branch & embryonic tracheole

      (Paul et al., 2003)

      tracheal dorsal trunk primordium

      (Paul et al., 2003)
      Detailed Description
      Statement
      Reference

      In nrv2nwu3 homozygous embryos, beginning at the time of tracheal tube expansion (stage 15), multicellular tracheal tubes become increasingly abnormal. By stage 16 the average dorsal trunk length is significantly greater than wild type (P<0.005) (113+/-2% mean+/-s.e.m., n>5, normalized to stage 16 wild-type value) and all tube diameters are irregular with expansions and constrictions along their lengths. Defects are also present in regions of single cell tubes formed by autocellular junctions, particularly near the ends of the ganglionic branches where there are lumenal staining discontinuities. nrv2nwu3/nrv223B embryos from nrv2nwu3 germline clone mothers are indistinguishable from nrv2nwu3 homozygotes with respect to their tracheal patterning. Unlike wild-type, post stage 15 trachea in nrv2nwu3 homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier.

      (Paul et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      NOT Enhancer of
      Statement
      Reference

      nrv2nwu3/nrv2nwu3 is a non-enhancer of presumptive embryonic/larval tracheal system phenotype of cora5

      (Paul et al., 2003)
      NOT Suppressor of
      Statement
      Reference

      nrv2nwu3/nrv2nwu3 is a non-suppressor of presumptive embryonic/larval tracheal system phenotype of cora5

      (Paul et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      sinunwu7 nrv2nwu3 double mutants have cyst-like structures in the tracheal ganglionic branches.

      (Wu et al., 2004)

      nrv2nwu3; cora5 embryos have the same phenotype as either single mutant. The tracheal phenotypes of nrv2nwu3; GliJ29Δ7b double homozygous embryos are not more severe than that of nrv2nwu3 homozygous embryos. The tracheal phenotypes of nrv2nwu3 homozygotes are exacerbated by convk06507b/convk06507b or mmyk13717b/mmyk13717b.

      (Paul et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      nrv2nwu3
      Name Synonyms
      Secondary FlyBase IDs
        References (2)