FB2026_02 , released June 18, 2026
Allele: Dmel\nrv223B
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General Information
Symbol
Dmel\nrv223B
Species
D. melanogaster
Name
FlyBase ID
FBal0151681
Feature type
allele
Associated gene
Dmel\nrv2
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Paul et al., 2003)
Mutagen
P-element activity
(Genova and Fehon, 2003)
Progenitor genotype
nrv2k13315
(Genova and Fehon, 2003)
Cytology
Description

Imprecise excision of P{lacW}nrv2k13315 has produced a deletion of 2176bp starting at the P{lacW}nrv2k13315 insertion site and encompassing all exons common to nrv2.1 and nrv2.2 alternative transcripts.

(Genova and Fehon, 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      embryonic ganglionic branch & embryonic tracheole

      (Paul et al., 2003)

      ganglionic tracheal branch primordium

      (Paul et al., 2007)

      septate junction & embryonic tracheal system

      (Paul et al., 2007)

      tracheal dorsal trunk primordium

      (Paul et al., 2007, Paul et al., 2003)
      Detailed Description
      Statement
      Reference

      Mutants have tracheal defects, including dorsal trunks that are increased in length compared to wild type and have an irregular diameter and lumenal staining gaps in ganglionic branches. In contrast to wild type, the paracellular barrier function of the septate junctions has also been lost in the trachea, allowing a dye to penetrate the epithelium and accumulate in the tracheal lumen.

      (Paul et al., 2007)

      In nrv223B homozygous embryos, tracheal phenotypes are apparent from stage 15. At stage 16 in these embryos the average dorsal trunk length is significantly greater than wild type (P<0.005) (126+/-2% mean+/-s.e.m., n>5, normalized to stage 16 wild-type value). In addition these embryos have moderately severe diameter increases in the dorsal trunk and other primary tracheal branches, and some ganglionic branches exhibit missing lumen. nrv2nwu3/nrv223B embryos from nrv2nwu3 germline clone mothers are indistinguishable from nrv2nwu3 homozygotes with respect to their tracheal patterning.

      (Paul et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      nrv223B has tracheal dorsal trunk primordium phenotype, non-suppressible by nrv1UAS.cPa/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by Scer\GAL4da.G32/nrv2::nrv3Nrv2AB.UAS

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv2::nrv3Nrv2A.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv2::nrv3Nrv2CD.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv2::nrv3Nrv2BCD.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by Scer\GAL4da.G32/nrv2::nrv3Nrv2D.UAS

      (Paul et al., 2007)

      nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv2::nrv3Nrv2ABC.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has septate junction & embryonic tracheal system phenotype, non-suppressible by nrv1UAS.cPa/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has ganglionic tracheal branch primordium phenotype, non-suppressible by nrv1UAS.cPa/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has tracheal dorsal trunk primordium phenotype, non-suppressible by Scer\GAL4da.G32/nrv3UAS.cPa

      (Paul et al., 2007)

      nrv223B has septate junction & embryonic tracheal system phenotype, non-suppressible by Scer\GAL4da.G32/nrv3UAS.cPa

      (Paul et al., 2007)

      nrv223B has ganglionic tracheal branch primordium phenotype, non-suppressible by Scer\GAL4da.G32/nrv3UAS.cPa

      (Paul et al., 2007)

      nrv223B has tracheal dorsal trunk primordium phenotype, non-suppressible by nrv2::nrv32IT.3E.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has septate junction & embryonic tracheal system phenotype, non-suppressible by nrv2::nrv32IT.3E.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv223B has ganglionic tracheal branch primordium phenotype, non-suppressible by nrv2::nrv32IT.3E.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)

      nrv2k13315/nrv223B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by Scer\GAL4e22c/nrv3UAS.cPa

      (Paul et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The tracheal defects of sinunwu7 mutant embryos are more severe in double mutant combination with nrv223B (expansion of the tube diameter is more severe in the transverse connective and visceral branches).

      (Wu et al., 2004)

      nrv2k13315/nrv223B tracheal phenotypes are not suppressed by nrv3Scer\UAS.cPa; Scer\GAL4e22c.

      (Paul et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      nrv223B is rescued by nrv21.UAS/Scer\GAL4da.G32

      (Paul et al., 2007)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Genova and Fehon

      (Paul et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      nrv223B
      (Stork et al., 2009)
      nrv223b
      (Wu et al., 2007)
      nrv2[23B]
      (Paul et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)