A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC785 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The breakpoints of Df(2R)BSC785 predicted from the Release 5 genomic coordinates of the progenitor P{XP}CG30185d11316 and PBac{RB}Pi3K59Fe03961 transposable element insertion sites are 2R:19431599 ;19447811 and the cytological breakpoints predicted from these coordinates are 59E3;59E4.