[27F3-27F3];[28C4-28C4];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
27F3;28C4
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Lethal in combination with Df(2L)ED479.
Fails to complement Df(2L)ED499.
Fails to complement Df(2L)Exel7031.
Inferred to overlap with: Df(2L)ED499.
Inferred to overlap with: Df(2L)Exel7031.
Presence of P+PBac{XP5.RB3}BSC189 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(2L)BSC189 predicted from the Release 4 genomic coordinates of the progenitor PBac{RB}CG5149e03983 and P{XP}d04880 insertion sites are 27F3;28C4.