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General Information
Symbol
Dmel\Camn339
Species
D. melanogaster
Name
FlyBase ID
FBal0048074
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Camnull, cam339
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Mobilisation of the P{lacW} insertion generating a deletion of exons 0 and 1 and the initiator codon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

bouton & abdominal 2 ventral longitudinal muscle 2

bouton & abdominal 3 ventral longitudinal muscle 2

bouton & abdominal 4 ventral longitudinal muscle 2

bouton & abdominal 5 ventral longitudinal muscle 2

bouton & abdominal 6 ventral longitudinal muscle 2

bouton & abdominal 7 ventral longitudinal muscle 2

Detailed Description
Statement
Reference

Camn339/+ flies have normal wing structure.

Sound-evoked compound action potentials in the antennal nerve are not significantly different from wild type in Cam5/Camn339 flies. However, nonlinear mechanical amplification is significantly increased compared to wild type.

Camn339 heterozygotes exhibit little effect on the average period of free-running.

Homozygous larvae are sluggish and show spontaneous backward locomotion. Cam7/Camn339 larvae are morphologically normal and show normal forward locomotion with no spontaneous backward movement. However, they are sluggish with a body wall contraction (BWC) rate about one-third that of controls. Cam7/Camn339 wandering third instar larvae become progressively incapable of relaxing at the end of each body-wall contraction and show increasing stiffness during locomotion. Third instar body wall muscles that have been treated with ether (to artificially relax them) all show longitudinal muscles that have a "bunched" appearance, with structural disorganisation and misaligned myofibrils. Cam7/Camn339 animals form pupal cases with deep indentations at the larval segment boundaries. On average the mutant larvae decrease their body length by 50% during pupariation, compared to a decrease of one-third in wild-type larvae. Only the long axis is abnormally compressed in the mutant animals. Cam7/Camn339 pupae never eclose, but most develop into pharate adults with head defects. Three types of heads are seen; normal heads with no obvious defects, malformed heads that are partially everted and "inside-out" heads where head eversion has completely failed and head development proceeds in a noneverted head sac buried in the thorax. There is a correlation between the pupal length:width ratio and the degree of head abnormality; the more severe head defects are associated with shorter pupal cases. Cam7/Camn339 animals rescued to adulthood by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B are behaviourally abnormal; they fail to climb after being gently knocked to the bottom of a vial, and they show poor flight and a reduced ability to right themselves after a brief vortexing. The initial stages of male courtship are normal, but the rescued males cannot bend their abdomens sufficiently to achieve penetration. The amount of backward movement seen in Cam03909/Camn339 larvae is increased by high light intensity. The amount of backward movement seen in Cam352/Camn339 larvae is increased by high light intensity.

Cam7/Camn339 pupal cases are shorter than wild type and have deeply indented rings at the larval segment boundaries. Many animals have head eversion defects and none eclose. Expression of both CamB12Q.Scer\UAS and CamB34Q.Scer\UAS under the control of Scer\GAL4how-24B in Cam7/Camn339 animals results in 100% larval lethality.

Cam3c1/Camn339 flies exhibit reduced locomotion, coordination and flight ability. A slight dominant effect can be detected for both Cam3c1 and Camn339. The recessive mutant effects are more substantial. In the larval neuromuscular junction voltage clamp preparation, working on muscle 6, ejc amplitude for Cam3c1/Camn339 is normal. Ejc amplitude is increased approximately three fold by quinidine at low external calcium concentrations (at 0.4mM external calcium quinidine has no effect. The mejc amplitude and frequency is unchanged, suggesting the increase in ejc reflects increased neurotransmitter release. Cam3c1/Camn339 larvae show structural synaptic abnormalities: the terminal arbor forms a thickened, or large, misshapen structure with few distinct boutons. This results in a reduced number of boutons and a nearly complete lack of terminal branching in pleural external longitudinal muscle 13. Muscle 13 shows the same ejc phenotype as ventral internal longitudinal muscle 6. No abnormalities in nerve terminals on muscles ventral internal longitudinal muscle 6, 7 or pleural external longitudinal muscle 12 have been observed.

Transheterozygous combinations with other Cam mutations produces an incompletely penetrant ectopic wing vein phenotype.

Camn339/Cam352 animals do not eclose from the pupal case. Camn339/Cam352 photoreceptors have dramatic defects in deactivation kinetics, displaying greatly prolonged deactivation times. Similar deactivation kinetics are seen at different concentrations of extracellular calcium ions, in contrast to wild-type. A single photon produces a train of quantum bumps in Cam352/Camn339 double mutant photoreceptors, in contrast to wild-type.

Maternal Cam supports Camn339 individuals throughout embryogenesis but they die within 2 days of hatching as first instar larvae. During feeding larvae show a high frequency of head swinging compared to heterozygous siblings. Larvae exhibit a significant decrease in the number of locomotive contraction waves due to a lower frequency of contraction initiation and most locomotion in the larvae is spontaneous backward movement. This spontaneous avoidance behaviour does not originate from any obvious morphological defects in the neuromuscular apparatus so starvation could be a potential cause of the aberrant locomotion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Cam7/Camn339 has lethal phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has lethal phenotype, suppressible | partially by Ca-α1DX7/Ca-alpha1D[+]

NOT suppressed by
Statement
Reference

Cam7/Camn339 has lethal phenotype, non-suppressible by Ca-α1DAR66/Ca-alpha1D[+]

Cam7/Camn339 has lethal phenotype, non-suppressible by Df(2R)H3E1/+

Cam7/Camn339 has lethal phenotype, non-suppressible by cn[+]/cn1

NOT Suppressor of
Statement
Reference

Cam[+]/Camn339 is a non-suppressor of visible phenotype of IP3K2wy-2

Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Cam7/Camn339 has adult head phenotype, non-enhanceable by cn[+]/cn1

Cam7/Camn339 has pupa phenotype, non-enhanceable by cn[+]/cn1

Suppressed by
Statement
Reference

Cam7/Camn339 has pupa phenotype, suppressible | partially by Ca-α1DAR66/Ca-alpha1D[+]

Cam7/Camn339 has adult head phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has pupa phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has pupal cuticle phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has adult head phenotype, suppressible | partially by Df(2R)H3E1/+

Cam7/Camn339 has pupa phenotype, suppressible | partially by Df(2R)H3E1/+

Cam7/Camn339 has pupa phenotype, suppressible | partially by Ca-α1DX7/Ca-alpha1D[+]

NOT suppressed by
Statement
Reference

Cam7/Camn339 has adult head phenotype, non-suppressible by cn[+]/cn1

Cam7/Camn339 has pupa phenotype, non-suppressible by cn[+]/cn1

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

Cam[+]/Camn339 is a non-suppressor of wing phenotype of IP3K2wy-2

Other
Additional Comments
Genetic Interactions
Statement
Reference

Camn339/+ does not modify wing phenotypes in IP3K2wy-2/IP3K2wy-2 flies.

Cam Dys double heterozygous flies (Camn339/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Camn339 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of Camn339 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Camn339 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

Camn339 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Camn339/+ mutant flies do not exhibit temperature-induced mobility defects.

Combination of Df(3R)Exel6184 in heterozygous state with a single copy of Camn339 results in a significantly increased frequency of lamina plexus defects in the third instar larval brain and reduced rhabdomere length in the adult eye in the double heterozygotes.

Combination of DgO86 in heterozygous state with a single copy of any of Camn339 results in significantly increased frequency of lamina plexus defects in third instar larvae and reduced rhabdomere length in the adults.

Rya-r44F16/+ rescues the pupal defects and lethality of Cam7/Camn339 animals; the pupae are smooth and indentation free in the anterior and only have mild ridges in the posterior, their length:width ratios are actually higher than wild type, more than 40% of the pupae eclose and those that do not eclose do not show head defects. Some of the eclosed animals do not show wing expansion and they all perform poorly in a climb test. Df(2R)H3E1/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; pupae show some increase in the pupal length:width ratio, but very few pupal cases have decreased indentations, and fewer head defects are seen in pupae, but they fail to eclose. Ca-α1DX7/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; there is a small but significant increase in the pupal length:width ratio, but no obvious effect on pupal case indentations, and a small fraction of the animals eclose as weak, uncoordinated adults with no wing expansion. Ca-α1DAR66/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; there is an increase in the pupal length:width ratio, but none of the pupae eclose.

In combination with l(2)C43C43 mutants exhibit completely penetrant lethality, mutants survive to pupation.

trp9; Cam352/Camn339 double mutant photoreceptors do not have transient light responses.

Xenogenetic Interactions
Statement
Reference

A heterozygous Camn339 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf has no effect on the length of the free-running period, compared to controls, after 1-5 weeks in constant darkness.

Complementation and Rescue Data
Comments

The lethality and pupariation defects seen in Cam7/Camn339 animals are rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B; the pupal cases are morphologically wild type and have more normal length:width ratios, almost all of the rescued pupae eclose and no aberrant pharate head structures are seen. The lethality and pupariation defects seen in Cam7/Camn339 animals are not rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4elav.PLu or Scer\GAL4sca.PU. The sluggish larval locomotion of Cam7/Camn339 animals is incompletely rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B or Scer\GAL4elav.PLu. The spontaneous backward movement seen in homozygous Camn339 larvae is not rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B or Scer\GAL4sca.PU. The spontaneous backward movement seen in homozygous Camn339 larvae is rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4elav.PLu, although the larvae are still sluggish.

The Camn339/Cam352 photoreceptor deactivation time phenotype can be partially rescued by Camhs.PDH, the degree of rescue depending on the number of heat shocks given.

Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (21)