FlyBase Allele Report: Dmel\RyR[16]

General Information

Symbol

Dmel\RyR¹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0117664

Feature type

allele

Associated gene

Dmel\RyR

Associated Insertion(s)

Carried in Construct

Also Known As

Rya-r44F¹⁶

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sullivan et al., 2000)

Progenitor genotype

RyR^k04913

(Sullivan, 2001.4.25, Sullivan et al., 2000)

Cytology

Description

RyR¹⁶ is caused by a 6kb internal deletion.

(Casas-Tinto et al., 2011)

1830 bp of genomic sequence is deleted downstream from the Rya-r44F^k04913 insertion. The deletion removes 1377 bp out of the 2847 bp second intron. A small insertion of 19 bp from the 5' end of the Rya-r44F^k04913 insertion remains.

(Sullivan, 2001.4.25)

Contains a deletion that extends from the P{lacW}Rya-r44F^k04913 insertion removing the second exon, including the translation start.

(Sullivan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:8,862,557..8,864,387 [+]

Comment:

1830 bp of genomic sequence is deleted downstream from the k04913 insertion including 1377 bp of the 2847 bp second intron. There is

also a small insertion of 19 bp from the 5' end of the k04913 P element.

(Sullivan, 2001.4.25)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

RyR¹⁶ homozygous embryos do not show any obvious somatic musculature phenotype, as compared to controls.

(Jacobs, 2018.7.9)

Reducing cytosolic Ca[2+] levels with one copy of RyR¹⁶ results in increased resistance to paraquat intoxication compared to controls. Increased survival rates are seen at 24 and 48 hours after ingestion.

(Cassar et al., 2015)

Heterozygous RyR¹⁶ mutant flies exhibit diminished cardiac function independent of heat shock. No heat-shock induced paralysis is observed and heart rate is similar to controls.

(Abraham and Wolf, 2013)

Homozygous RyR¹⁶ embryos are lethal and show slow feeding, locomotion and heart rate. Flies heterozygous for RyR¹⁶ exhibit normal eyes and viability.

(Casas-Tinto et al., 2011)

Body wall contraction (BWC) rates of homozygous larvae on an agar substrate are significantly reduced compared to wild type.

(Gao et al., 2004)

Homozygous Rya-r44F¹⁶ larvae appear to develop normally, but appear smaller and rounder than wild-type, and the head does not extend properly. When Body Wall Contractions (BWCs) are assayed in homozygous Rya-r44F¹⁶ larvae, the timing of BWC initiation appears normal, but contraction propagate more slowly than in wild-type, such that the rate of BWC is reduced by 50%. Furthermore contraction in these animals is attenuated and often has associated tremors. Mutant larvae ingest their food much more slowly than wild-type and accumulate food in the pharynx. They also fail to excrete and defecate digested food much more slowly than wild-type. In Rya-r44F¹⁶ homozygous larvae, the heart rate is reduced by about 75% relative to heterozygotes. This is caused by loss of fast fibrillating contractions but not an increase in pausing. the overall behaviour of homozygous Rya-r44F¹⁶ larvae is indistinguishable from wild-type; they detect and migrate to food sources, have an enhanced rate of food ingestion after starvation, have normal salt avoidance and response to mechanical stimulation and show no spontaneous avoidance behaviour. BrdUrd labelling of Rya-r44F¹⁶ brains show that cell cycle progression is normal in these mutants. About 60% of Rya-r44F¹⁶/Df(2R)Np3 embryos fail to hatch. In larvae, the rate of BWC is reduced by 90%. In homozygous mutant clones in the eye appear morphologically normal, and whole cell, voltage clamp recordings on ommatidia show responses indistinguishable from wild-type. The progeny of homozygous germline clones are also indistinguishable from wild-type.

(Sullivan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

RyR¹⁶ has abnormal neuroanatomy | third instar larval stage | dominant phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

NOT suppressed by

Statement

Reference

RyR¹⁶ has abnormal neuroanatomy | dominant | third instar larval stage phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

Enhancer of

Statement

Reference

RyR¹⁶/RyR[+] is an enhancer | female limited of partially lethal - majority die phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

NOT Enhancer of

Statement

Reference

RyR¹⁶ is a non-enhancer of abnormal neuroanatomy | third instar larval stage | dominant phenotype of iav^hypoB-1

(Wong et al., 2014)

Suppressor of

Statement

Reference

RyR¹⁶/RyR[+] is a suppressor of abnormal heat stress response | dominant phenotype of SERCA^Kum170

(Abraham and Wolf, 2013)

RyR¹⁶/RyR[+] is a suppressor of increased mortality during development phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav-C155

(Casas-Tinto et al., 2011)

RyR¹⁶/Rya-r44F[+] is a suppressor of lethal phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

NOT Suppressor of

Statement

Reference

RyR¹⁶/RyR[+] is a non-suppressor of paralytic | dominant phenotype of SERCA^Kum170

(Abraham and Wolf, 2013)

Other

Statement

Reference

Cam⁷/Camⁿ³³⁹, RyR¹⁶ has abnormal locomotor behavior phenotype

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹, RyR¹⁶/Rya-r44F[+] has abnormal locomotor behavior phenotype

(Wang et al., 2003)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

RyR¹⁶ has NMJ bouton | third instar larval stage | decreased number phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

RyR¹⁶ has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

NOT suppressed by

Statement

Reference

RyR¹⁶ has NMJ bouton | third instar larval stage | decreased number phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

RyR¹⁶ has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

NOT Enhancer of

Statement

Reference

RyR¹⁶ is a non-enhancer of NMJ bouton | third instar larval stage | decreased number phenotype of iav^hypoB-1

(Wong et al., 2014)

RyR¹⁶ is a non-enhancer of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of iav^hypoB-1

(Wong et al., 2014)

Suppressor of

Statement

Reference

RyR¹⁶/RyR[+] is a suppressor of adult heart phenotype of SERCA^Kum170

(Abraham and Wolf, 2013)

RyR¹⁶/RyR[+] is a suppressor of eye phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Casas-Tinto et al., 2011)

RyR¹⁶/RyR[+] is a suppressor of retina phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Casas-Tinto et al., 2011)

RyR¹⁶/RyR[+] is a suppressor of photoreceptor neuron phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Casas-Tinto et al., 2011)

RyR¹⁶/Rya-r44F[+] is a suppressor of adult head phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

RyR¹⁶/Rya-r44F[+] is a suppressor of pupa phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

RyR¹⁶/Rya-r44F[+] is a suppressor of pupal cuticle phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

Other

Statement

Reference

Cam⁷/Camⁿ³³⁹, RyR¹⁶ has wing phenotype

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹, RyR¹⁶/Rya-r44F[+] has wing phenotype

(Wang et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

RyR¹⁶ partially suppresses the phenotypes seen in heterozygous Ca-P60A^Kum170 mutant flies in response to heat shock. The paralysis is still present but the increase in heart rate and defect in cardiac function are both suppressed. End diastolic dimensions and fractional shortening are also unchanged compared to Ca-P60A^Kum170 alone.

(Abraham and Wolf, 2013)

Rya-r44F¹⁶/+ rescues the pupal defects and lethality of Cam⁷/Camⁿ³³⁹ animals; the pupae are smooth and indentation free in the anterior and only have mild ridges in the posterior, their length:width ratios are actually higher than wild type, more than 40% of the pupae eclose and those that do not eclose do not show head defects. Some of the eclosed animals do not show wing expansion and they all perform poorly in a climb test.

(Wang et al., 2003)

Xenogenetic Interactions

Statement

Reference

A heterozygous RyR¹⁶ background significantly suppresses the eye degeneration seen upon expression of Hsap\APP^{2xAPP.SP.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Sixty percent of the flies expressing only Hsap\APP^{2xAPP.SP.Scer\UAS} show a highly disorganised eye surface and short retina with small photoreceptors. On the other hand, 62% of the flies with a heterozygous RyR¹⁶ background exhibit mild eye disorganisation with well-developed retinae and long photoreceptors. There is a significant improvement in eye organisation in flies carrying RyR¹⁶.

Viability of flies expressing Hsap\APP^{2xAPP.SP.Scer\UAS} in the CNS (under the control of Scer\GAL4^elav-C155) is completely restored in a heterozygous RyR¹⁶ background.

(Casas-Tinto et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6812

y¹ w^*; RyR¹⁶/CyO, y⁺

Notes on Origin

Discoverer

Comments

Phenotypic analysis suggest the allelic series: Df(2R)Np3 > Rya-r44F¹⁶ > Rya-r44F^k04913.

(Sullivan et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

RyR16

(Richardson et al., 2022)

RyR¹⁶

(Wong et al., 2021, Park et al., 2020, Brusich et al., 2018, Cassar et al., 2015, Wong et al., 2014)

Rya-44F¹⁶

(Abraham and Wolf, 2013)

Rya-R44F¹⁶

(Jacobs, 2018.7.9)

Rya-r44F¹⁶

(Abraham and Wolf, 2013)

Rya-r¹⁶

(Casas-Tinto et al., 2011)

Ryr¹⁶

(Gao et al., 2004, Wang et al., 2003, Sullivan et al., 2000)

rya-r44F¹⁶

(Van Goethem et al., 2012)

ryr¹⁶

(Sullivan, 2001.4.25)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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