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General Information
Symbol
Dmel\Cam7
Species
D. melanogaster
Name
FlyBase ID
FBal0083596
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T12269289G

Amino acid change:

V92G | Cam-PA; V92G | Cam-PB; V92G | Cam-PC; V92G | Cam-PD; V92G | Cam-PE

Reported amino acid change:

V91G

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: V91G.

Amino acid replacement: V91G. Alters a residue in the N-terminal helix of binding site 3.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cam7/+ flies have normal wing structure.

Cam7 heterozygotes exhibit little effect on the average period of free-running.

Cam7/Camn339 larvae are morphologically normal and show normal forward locomotion with no spontaneous backward movement. However, they are sluggish with a body wall contraction (BWC) rate about one-third that of controls. Cam7/Camn339 wandering third instar larvae become progressively incapable of relaxing at the end of each body-wall contraction and show increasing stiffness during locomotion. Third instar body wall muscles that have been treated with ether (to artificially relax them) all show longitudinal muscles that have a "bunched" appearance, with structural disorganisation and misaligned myofibrils. Cam7/Camn339 animals form pupal cases with deep indentations at the larval segment boundaries. On average the mutant larvae decrease their body length by 50% during pupariation, compared to a decrease of one-third in wild-type larvae. Only the long axis is abnormally compressed in the mutant animals. Cam7/Camn339 pupae never eclose, but most develop into pharate adults with head defects. Three types of heads are seen; normal heads with no obvious defects, malformed heads that are partially everted and "inside-out" heads where head eversion has completely failed and head development proceeds in a noneverted head sac buried in the thorax. There is a correlation between the pupal length:width ratio and the degree of head abnormality; the more severe head defects are associated with shorter pupal cases. Cam7/Camn339 animals rescued to adulthood by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B are behaviourally abnormal; they fail to climb after being gently knocked to the bottom of a vial, and they show poor flight and a reduced ability to right themselves after a brief vortexing. The initial stages of male courtship are normal, but the rescued males cannot bend their abdomens sufficiently to achieve penetration.

Cam7/Camn339 pupal cases are shorter than wild type and have deeply indented rings at the larval segment boundaries. Many animals have head eversion defects and none eclose. Expression of CamB34Q.Scer\UAS under the control of Scer\GAL4how-24B in Cam7/+ animals (which are normally phenotypically wild type) results in less than half the animals pupariating and some pupae have body wrinkles similar to that seen in Cam7 hemizygotes. Expression of both CamB12Q.Scer\UAS and CamB34Q.Scer\UAS under the control of Scer\GAL4how-24B in Cam7/Camn339 animals results in 100% larval lethality.

Mutant larvae are sluggish and form highly indented pupal cases as a result of excessive longitudinal contraction during pupariation. Many pupae have problems with head eversion and formation and no adults eclose.

Hemizygotes survive to pupate but never emerge from the pupal cases. The pupal cases are smaller than normal with distinctive 'Michelin-man' phenotype of deeply indented rings corresponding to junctions of the larval body segments. Most individuals the develop within the pupal case are headless or have partial head structures due to a failure of head eversion. Inverted head structures, including fully formed eyes, can be dissected out of the thoraces. Some individuals have fully everted heads. Cam7/Cam8t mutants also die in the pupal case but few develop far enough to show failed head eversion. Camhs.PN restores good viability, about 50%. Transheterozygous combinations with other Cam mutations produces an incompletely penetrant ectopic wing vein phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Cam7/Camn339 has lethal phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has lethal phenotype, suppressible | partially by Ca-α1DX7/Ca-alpha1D[+]

NOT suppressed by
Statement
Reference

Cam7/Camn339 has lethal phenotype, non-suppressible by Ca-α1DAR66/Ca-alpha1D[+]

Cam7/Camn339 has lethal phenotype, non-suppressible by Df(2R)H3E1/+

Cam7/Camn339 has lethal phenotype, non-suppressible by cn[+]/cn1

Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

Cam7/Cam[+] is a non-suppressor of visible phenotype of IP3K2wy-2

Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Cam7/Camn339 has adult head phenotype, non-enhanceable by cn[+]/cn1

Cam7/Camn339 has pupa phenotype, non-enhanceable by cn[+]/cn1

Suppressed by
Statement
Reference

Cam7/Camn339 has pupa phenotype, suppressible | partially by Ca-α1DAR66/Ca-alpha1D[+]

Cam7/Camn339 has adult head phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has pupa phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has pupal cuticle phenotype, suppressible by RyR16/Rya-r44F[+]

Cam7/Camn339 has adult head phenotype, suppressible | partially by Df(2R)H3E1/+

Cam7/Camn339 has pupa phenotype, suppressible | partially by Df(2R)H3E1/+

Cam7/Camn339 has pupa phenotype, suppressible | partially by Ca-α1DX7/Ca-alpha1D[+]

NOT suppressed by
Statement
Reference

Cam7/Camn339 has adult head phenotype, non-suppressible by cn[+]/cn1

Cam7/Camn339 has pupa phenotype, non-suppressible by cn[+]/cn1

NOT Suppressor of
Statement
Reference

Cam7/Cam[+] is a non-suppressor of wing phenotype of IP3K2wy-2

Other
Statement
Reference

Ca-α1DX7/Ca-alpha1D[+], Cam7/Camn339 has wing phenotype

Cam7/Camn339, RyR16/Rya-r44F[+] has wing phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Cam7/+ does not modify wing phenotypes in IP3K2wy-2/IP3K2wy-2 flies.

Rya-r44F16/+ rescues the pupal defects and lethality of Cam7/Camn339 animals; the pupae are smooth and indentation free in the anterior and only have mild ridges in the posterior, their length:width ratios are actually higher than wild type, more than 40% of the pupae eclose and those that do not eclose do not show head defects. Some of the eclosed animals do not show wing expansion and they all perform poorly in a climb test. Df(2R)H3E1/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; pupae show some increase in the pupal length:width ratio, but very few pupal cases have decreased indentations, and fewer head defects are seen in pupae, but they fail to eclose. Ca-α1DX7/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; there is a small but significant increase in the pupal length:width ratio, but no obvious effect on pupal case indentations, and a small fraction of the animals eclose as weak, uncoordinated adults with no wing expansion. Ca-α1DAR66/+ has a slight suppressing effect on the Cam7/Camn339 phenotype; there is an increase in the pupal length:width ratio, but none of the pupae eclose.

In combination with l(2)C43C43 mutants exhibit markedly reduced viability.

Xenogenetic Interactions
Statement
Reference

A heterozygous Cam7 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf can result in lengthening of the free-running period, compared to controls, after 3-5 weeks in constant darkness.

Complementation and Rescue Data
Comments

The lethality and pupariation defects seen in Cam7/Camn339 animals are rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B; the pupal cases are morphologically wild type and have more normal length:width ratios, almost all of the rescued pupae eclose and no aberrant pharate head structures are seen. The lethality and pupariation defects seen in Cam7/Camn339 animals are not rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4elav.PLu or Scer\GAL4sca.PU. The sluggish larval locomotion of Cam7/Camn339 animals is incompletely rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B or Scer\GAL4elav.PLu.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Allelic series of the relative strength of the lethal mutations: Cam8t > Cam7 > Cam6c4 > Cam5 > Cam4c1 > Cam3c1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
  • FBal0104882
References (7)