FlyBase Allele Report: Dmel\Ca-α1D[AR66]

General Information

Symbol

Dmel\Ca-α1D^AR66

Species

D. melanogaster

Name

FlyBase ID

FBal0033484

Feature type

allele

Associated gene

Dmel\Ca-α1D

Associated Insertion(s)

Carried in Construct

Also Known As

Dmca1D^AR66

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Eberl et al., 1998)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Roote, 1995.5.4)

Progenitor genotype

Cytology

Description

Amino acid replacement: C629Y. Residue resides in the transmembrane domain I-S1 closer to the extracellularside.

(Eberl et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,173,759..16,173,759 [+]

Nucleotide change:

G16173759A

Amino acid change:

(Eberl et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage

(Hsu et al., 2020)

abnormal neuroanatomy

(Lee and Wu, 2010)

lethal | recessive

(Ashburner et al., 1990)

lethal - all die before end of P-stage | recessive

(Eberl et al., 1998)

some die during pharate adult stage | recessive

(Eberl et al., 1998)

Phenotype Manifest In

larval abdominal segmental nerve

(Eberl et al., 1998)

larval longitudinal connective

(Eberl et al., 1998)

larval RP2 motor neuron | larval stage

(Hsu et al., 2020)

neuromuscular junction

(Lee and Wu, 2010)

NMJ bouton

(Lee and Wu, 2010)

type Is terminal bouton | larval stage

(Hsu et al., 2020)

wing

(Eberl et al., 1998)

Detailed Description

Statement

Reference

Ca-α1D^AR66 third instar larvae exhibit decreased locomotion.

Ca-α1D^AR66 larvae exhibit a decrease in neuropeptide (Dilp2-GFP) release in type I boutons (on muscle 4) upon activation of RP2 motor neuron via exposure to high potassium.

(Hsu et al., 2020)

In uncrowded conditions 48% eclose as adults although development is delayed by 1-2 days. Wings are usually unexpanded and many flies are found stuck in the food. The remaining 52% have difficulty eclosing and die as pharate adults. Transheterozygotes with Ca-α1D^X7 are almost all able to escape the puparium but few transheterozygotes with Ca-α1D^X10 succeed. A few homozygotes exhibit neurons in the longitudinal tracts that appear to stall at some of the commissures, forming nodular growths. Motoneurons in the SNb branch of the developing PNS also show stalling at stage 17. The defects may be due to a second lesion on the chromosome or generated by altered channel properties caused by the missense mutation.

(Eberl et al., 1998)

Two-electrode voltage clamping of the larval body wall muscles demonstrates the total current is reduced in homozygotes, current is intermediate with wild type in heterozygotes. Total current is comprised of the A- and D-current, the D-current is significantly reduced by application of amiloride or inactivation of the A-current, A-currents remain unaffected. Mutant also exhibits a slowing of channel activation kinetics.

(Ren et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ca-α1D^AR66 is a suppressor of abnormal neuroanatomy phenotype of sei²

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of abnormal neuroanatomy phenotype of slo⁹⁸

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of abnormal neuroanatomy phenotype of slo¹

(Lee and Wu, 2010)

NOT Suppressor of

Statement

Reference

Ca-α1D^AR66/Ca-alpha1D[+] is a non-suppressor of lethal phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ca-α1D^AR66 is a suppressor of neuromuscular junction phenotype of slo¹

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of NMJ bouton | increased number phenotype of sei²

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of neuromuscular junction phenotype of sei²

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of NMJ bouton | increased number phenotype of slo⁹⁸

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of neuromuscular junction phenotype of slo⁹⁸

(Lee and Wu, 2010)

Ca-α1D^AR66 is a suppressor of NMJ bouton | increased number phenotype of slo¹

(Lee and Wu, 2010)

Ca-α1D^AR66/Ca-alpha1D[+] is a suppressor | partially of pupa phenotype of Cam⁷/Camⁿ³³⁹

(Wang et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

A Ca-α1D^AR66 mutant background suppresses both type B and type M satellites in Ca-α1D^AR66; slo¹ or Ca-α1D^AR66; slo⁹⁸ double mutants nearly to wild-type levels. Such suppression in satellite formation leads to the morphology of these double mutants to resemble Ca-α1D^AR66 single mutants.

A Ca-α1D^AR66 mutant background suppresses both type B and type M satellites in Ca-α1D^AR66; sei² double mutants nearly to wild-type levels. Such suppression in satellite formation leads to the morphology of these double mutants to resemble Ca-α1D^AR66 single mutants.

(Lee and Wu, 2010)

Ca-α1D^AR66/+ has a slight suppressing effect on the Cam⁷/Camⁿ³³⁹ phenotype; there is an increase in the pupal length:width ratio, but none of the pupae eclose.

(Wang et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ca-α1D^AR66 is rescued by Ca-α1D^+tCa01

(Eberl et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

S. Roth.

(Roote, 1995.5.4)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ca-α1D^AR66

(Trombley et al., 2023, Liu et al., 2022, Terada et al., 2016)

Dmca1D^AR66

(Hsu et al., 2020, Lee and Wu, 2010, Eberl et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close