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General Information
Symbol
Mmmm\PVUAS.Tag:MYC
Species
m. unknown
Name
FlyBase ID
FBal0217422
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PV
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Mmmm\PV coding sequences tagged with Tag:MYC and a mammalian nuclear export signal (to ensure accumulation in the cytoplasm).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mmmm\PVScer\UAS.T:Hsap\MYC expressing pre-synaptic terminals (expression under the control of Scer\GAL4elav-C155) exhibit altered Ca2+ dynamics compared with non-expressing controls. The transient Ca2+ increase induced by a single pre-synaptic action potential triggered by electrical stimulation of the motor nerve is smaller in Mmmm\PVScer\UAS.T:Hsap\MYC expressing terminals. The sustained Ca2+ increaase induced by a 10Hz train of action potentials is also quite different in Mmmm\PVScer\UAS.T:Hsap\MYC expressing terminals: the time to reach peak Ca2+ and the time constant of decay are each much longer in Mmmm\PVScer\UAS.T:Hsap\MYC expressing terminals.

Flies expressing 2 copies of Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf have free-running periods slightly shorter than 24 hours, and these periods do not change over the course of 4 weeks in dark/dark conditions. In contrast, Scer\GAL4P2.4.Pdf expression of 4 and 6 copies of Mmmm\PVScer\UAS.T:Hsap\MYC results in longer free-running periods of just under 25 hours during the first week in dark/dark. Over the course of 4 weeks in dark/dark conditions, the free-running period of the 6x Mmmm\PVScer\UAS.T:Hsap\MYC flies continues to lengthen to over 27 hours by the fourth week. Interestingly, approximately 20% of these flies shift to a weak short-period rhythm during the fourth week in dark/dark.

After release into dark/dark conditions after 3 weeks in light/dark conditions, flies expressing 6 copies of Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf immediately begin to free run with a period of approximately 27 hours, whereas 8x Mmmm\PVScer\UAS.T:Hsap\MYC flies free run with a period of approximately 24 hours.

Although Scer\GAL4P2.4.Pdf-driven expression of 6 copies of Mmmm\PVScer\UAS.T:Hsap\MYC results in the exhibition of robust 'morning' and 'evening' circadian anticipatory peaks of locomotor activity, increases are phase delayed relative to controls. There is a slight difference in the percentage of total activity that occurs in the dark versus the light, with Scer\GAL4P2.4.Pdf > 6x Mmmm\PVScer\UAS.T:Hsap\MYC flies engaging in more of their activity in the dark than controls.

Scer\GAL4P2.4.Pdf-driven expression of 6 copies of Mmmm\PVScer\UAS.T:Hsap\MYC results in significantly larger anticipation phase scores than controls for both the lights-on and lights-off transitions, indicating phase delays of activity increases for both morning and evening anticipation.

Expression of Mmmm\PVScer\UAS.T:Hsap\MYC in all clock neurons (under the control of Scer\GAL4tim.Scer\UAS) severely disrupts free-running rhythms of locomotor activity. These severe phenotypes include arrhythmicity and a change in behavior so no beam crossings are detected for a number of consecutive days, followed by resumption of substantial numbers of beam crossings. The average free-running period of rhythmic 2x Scer\GAL4tim.Scer\UAS > 4x Mmmm\PVScer\UAS.T:Hsap\MYC flies on the third week in dark/dark is 25.5 hours, approximately 2 hours longer than controls.

Pan-neuronal expression of Mmmm\PVScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4elav-C155 does not induce any lethality, reduced lifespan, or other gross behavioral phenotypes.

Expression of Mmmm\PVScer\UAS.T:Hsap\MYC in the LNvs, under the control of Scer\GAL4P2.4.Pdf does not abolish the cyclic release of Pdf from their dorsomedial terminals.

Expression of Mmmm\PVScer\UAS.T:Hsap\MYC in the LNvs, under the control of Scer\GAL4P2.4.Pdf has little or no effect on free-running circadian period.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Flies expressing both Mmmm\PVScer\UAS.T:Hsap\MYC and Rat\CamKII-IAla.hs under the control of Scer\GAL4P2.4.Pdf exhibit period lengthening that is significantly different from controls.

A heterozygous Cam4c1 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf can result in lengthening of the free-running period, compared to controls, after 3-4 weeks in constant darkness.

A heterozygous Cam5 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf can result in lengthening of the free-running period, compared to controls, after 5 weeks in constant darkness.

A heterozygous Cam7 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf can result in lengthening of the free-running period, compared to controls, after 3-5 weeks in constant darkness.

A heterozygous Cam3c1 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf has no effect on the length of the free-running period, compared to controls, after 1-5 weeks in constant darkness.

A heterozygous Camn339 background in flies expressing Mmmm\PVScer\UAS.T:Hsap\MYC under the control of Scer\GAL4P2.4.Pdf has no effect on the length of the free-running period, compared to controls, after 1-5 weeks in constant darkness.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Mmmm\PVScer\UAS.T:Hsap\MYC
Mmmm\PVUAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (4)