FlyBase Allele Report: Dmel\EcR[Q50st]

General Information

Symbol

Dmel\EcR^Q50st

Species

D. melanogaster

Name

FlyBase ID

FBal0083488

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

Also Known As

EcR^Q50

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choi et al., 2006)

Mutagen

ethyl methanesulfonate

(Bender et al., 1997)

Progenitor genotype

Cytology

Description

Mutation is within the EcR-B1 specific exon 2.

(Bender et al., 1997)

Nucleotide substitution: C?T.

(Bender et al., 1997)

Amino acid replacement: Q50term.

(Bender et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:6,123,413..6,123,413 [-]

Nucleotide change:

C6123413T

Reported nucleotide change:

C?T

Amino acid change:

Q50term | EcR-PB; Q50term | EcR-PG

Reported amino acid change:

Q50term

(Bender et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)ED1552)

(Kahsai et al., 2016)

lethal | recessive

(Bender et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

One copy of EcR^Q50st has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

Mutants that lack EcR-B1 (e.g. EcR^Q50st/EcR³¹) exhibit no difference in Crz-expressing ventral nerve cord neuronal cell death compared to wild-type.

(Choi et al., 2006)

Some EcR^Q50st/EcR^M554fs animals survive to the third larval instar stage, but they do not pupariate. They initiate wandering, but fail to evert spiracles, shorten, attach themselves to a solid surface, or harden their cuticle. The larvae easily slip free of their cuticle when dissected, indicating that apolysis of the larval cuticle has been completed.

(Bender et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

EcR[+]/EcR^Q50st is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

NOT Suppressor of

Statement

Reference

EcR[+]/EcR^Q50st is a non-suppressor of increased cell number | third instar larval stage phenotype of hop^Tum

(Sinenko et al., 2010)

Other

Statement

Reference

Df(2L)let-7-C^KO1, EcR^Q50st, P{W8,let7C^Δmir-let7} has abnormal neuroanatomy phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has abnormal neuroanatomy phenotype

(Kucherenko et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

EcR[+]/EcR^Q50st is a suppressor of wing vein phenotype of G9a^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

EcR[+]/EcR^Q50st is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

NOT Suppressor of

Statement

Reference

EcR[+]/EcR^Q50st is a non-suppressor of embryonic/larval hemocyte | third instar larval stage phenotype of hop^Tum

(Sinenko et al., 2010)

Other

Statement

Reference

Df(2L)let-7-C^KO1, EcR^Q50st, P{W8,let7C^Δmir-let7} has lobe system of adult mushroom body phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has lobe system of adult mushroom body phenotype

(Kucherenko et al., 2012)

EcR[+]/EcR^Q50st, usp⁴ has adult mushroom body beta-lobe phenotype

(Kucherenko et al., 2012)

EcR^Q50st, tai^61G1 has female germline stem cell | increased number phenotype

(König et al., 2011)

EcR^Q50st, tai^61G1 has germarium cap cell | increased number phenotype

(König et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The G9a^Scer\UAS.cKa wing vein phenotype is almost completely suppressed by a heterozygous EcR^Q50st background.

(Kleefstra et al., 2012)

Df(2L)let-7-C^KO1/EcR^Q50st ; P{W8,let-7-C^Δlet-7} animals have defects in the mushroom body α/β lobes: the lobes may appear slim or underdeveloped, axonal projections may be misguided.

usp⁴/+ ; EcR^Q50st/+ animals have defects in the mushroom body α/β lobes: the lobes may appear slim or the β lobes may be fused.

(Kucherenko et al., 2012)

EcR^Q50st/tai^61G1 germaria have additional germline stem cells and enlarged niches compared to wild type.

(König et al., 2011)

One copy of EcR^Q50st is unable to suppress the hemocyte overproliferation seen in hop^Tum mutant third instar larvae.

(Sinenko et al., 2010)

Xenogenetic Interactions

Statement

Reference

One copy of EcR^Q50st strongly suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4895

EcR^Q50st/SM6b

Kyoto

107935