cytochrome c, DC4, Cyt c, Cytc, Cyt-c2
Gene model reviewed during 5.47
Gene model reviewed during 5.55
Binds 1 heme group per subunit.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyt-c-p using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Cyt-c-p in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Cyt-c-p is neither necessary nor sufficient for caspase activation and apoptosis in Drosophila cells.
Mutating the invariant proline-30 of Cyt-c-p to alanine destabilizes the haem crevice more than the overall conformation.
The Cyt-c-p protein was detected as a functional cytochrome molecule and the amino acid sequence determined.
Cyt-c-p encodes a functional cytochrome c protein. Cyt-c-p is expressed at varying relatively high levels throughout development and the predicted polypeptide sequence differs from Cyt-c-d at 32 amino acid residues. Cyt-c-d and Cyt-c-p are located 3.5kb apart and have arisen from an ancient duplication event.
A cDNA clone for the thoracic muscle cytochrome c gene from M.sexta has been used to isolate the corresponding major thoracic muscle cytochrome c gene from D.melanogaster. Hybridisation studies reveal that Cyt-c-p is expressed at constant high levels. The genomic clone has been sequenced and its amino acid sequence derived. Cyt-c-d shares limited nucleotide homology with Cyt-c-p.
Apparently the structural gene for one of two cytochrome-c type enzymes, which differ from each other in 32/107 amino-acid residues. First isolated from a Charon-4 library using mouse cytochrome-c gene sequence as a probe. Cyt-c-d (sequence D3) expressed at constant, but relatively low, level throughout development; Cyt-c-p (sequence D4) expressed at varying, but relatively high, levels throughout development. Amino acid sequence also given in Fasman (1977).