FlyBase Allele Report: Dmel\tsr[N96A]

General Information

Symbol

Dmel\tsr^N96A

Species

D. melanogaster

Name

FlyBase ID

FBal0177371

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Johnson et al., 2008, Ng and Luo, 2004)

Mutagen

P-element activity

(Ng and Luo, 2004)

Progenitor genotype

Cytology

Description

P-element-mediated imprecise excision of the tsr locus.

(Ng and Luo, 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nemaline myopathy 7

CEA

(Balakrishnan et al., 2020)

model of autism spectrum disorder

CEA

(Sun et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | dominant | larval stage

(Sun et al., 2023)

abnormal neuroanatomy | somatic clone

(Ng and Luo, 2004)

abnormal size | somatic clone | third instar larval stage

(Ko et al., 2016)

increased cell death | somatic clone | third instar larval stage

(Ko et al., 2016)

Phenotype Manifest In

adult mushroom body & axon | somatic clone

(Ng and Luo, 2004)

adult mushroom body | somatic clone

(Ng and Luo, 2004)

embryonic/larval neuromuscular junction | larval stage

(Sun et al., 2023)

filamentous actin | larval stage

(Sun et al., 2023)

larval somatic muscle cell | decreased number | embryonic stage

(Balakrishnan et al., 2020)

wing disc | somatic clone | third instar larval stage

(Ko et al., 2016)

Detailed Description

Statement

Reference

tsr^N96A homozygous stage 16 embryos show muscle defects, including missing muscles; however, in the muscles that are present, sarcomeres are formed by embryonic stage 17, similar to controls.

(Balakrishnan et al., 2020)

tsr^N96A mutant clones within mosaic third instar larval wing discs are severely smaller than control clones; these clones show a strong increase in mitotic index (Casp3 and MMP1 immunofluorescence), as compared to controls.

(Ko et al., 2016)

Homozygous mushroom body neuroblast clones that are generated in newly hatched larvae contain only 15-30 neurons in the adult, in contrast to wild-type control clones, which contain approximately 400 neurons. The neuroblasts in the homozygous clones also show severe axon growth defects, with the majority of mutant neurons failing to extend their axons beyond the peduncle. Single cell homozygous clones of γ neurons in the mushroom body show axon growth defects in the adult; 30-40% of axons do not enter the medial lobe, stopping before the α/β branch point, 35-40% fail to extend beyond the midpoint of the γ lobe and 15-20% fail to reach the end of the γ lobe. Most of the mutant axons have multiple small protrusions and swellings along the the axon shaft and at the terminal, reminiscent of filopodia and lamellipodia normally associated with developing growth cones.

(Ng and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tsr[+]/tsr^N96A is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Suppressor of

Statement

Reference

tsr[+]/tsr^N96A is a suppressor of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A is a suppressor of abnormal cell migration phenotype of Rho1^UAS.cMa, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A is a suppressor of abnormal cell migration phenotype of Scer\GAL4^ptc-559.1, hep^UAS.cUa

(Rudrapatna et al., 2014)

Other

Statement

Reference

Mtl[+]/Mtl^Δ, Nav^Δ, Rac1^J11, Rac2^Δ/Rac2[+], tsr[+]/tsr^N96A has abnormal neuroanatomy phenotype

(Abe et al., 2014)

Csk^GD9345, tsr^N96A has lethal phenotype

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A, twf¹¹⁰ has visible phenotype

(Wang et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

tsr[+]/tsr^N96A is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

tsr[+]/tsr^N96A is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

tsr[+]/tsr^N96A is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Suppressor of

Statement

Reference

tsr[+]/tsr^N96A is a suppressor of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A is a suppressor of wing disc phenotype of Rho1^UAS.cMa, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A is a suppressor of wing disc phenotype of Scer\GAL4^ptc-559.1, hep^UAS.cUa

(Rudrapatna et al., 2014)

tsr[+]/tsr^N96A is a suppressor | partially of eye phenotype of mbt^P1

(Menzel et al., 2007)

tsr[+]/tsr^N96A is a suppressor of eye phenotype of mbt^P3

(Menzel et al., 2007)

Other

Statement

Reference

Mtl[+]/Mtl^Δ, Nav^Δ, Rac1^J11, Rac2^Δ/Rac2[+], tsr[+]/tsr^N96A has mushroom body pedunculus phenotype

(Abe et al., 2014)

Mtl[+]/Mtl^Δ, Nav^Δ, Rac1^J11, Rac2^Δ/Rac2[+], tsr[+]/tsr^N96A has adult mushroom body beta-lobe phenotype

(Abe et al., 2014)

Mtl[+]/Mtl^Δ, Nav^Δ, Rac1^J11, Rac2^Δ/Rac2[+], tsr[+]/tsr^N96A has adult mushroom body alpha-lobe phenotype

(Abe et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

sick^Δ tsr^N96A double heterozygotes do not show obvious axonal growth defects in α/β axonal lobes, peduncle and ellipsoid body.

The mushroom body axons of flies that are heterozygous for Rac1^J11, Rac2^Δ tsr^N96A sick^Δ and Mtl^Δ fail to extend to form peduncles and lobe structures (the 'posterior arrest' phenotype).

(Abe et al., 2014)

A tsr^N96A heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

A tsr^N96A/+ background suppresses the cell migration seen upon expression of Rho1^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1.

A tsr^N96A/+ background suppresses the cell migration seen upon expression of hep^Scer\UAS.cUa under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

tsr^N96A/+; twf¹¹⁰/+ transheterozygotes show mildly disorganised ridges and hooked tips.

(Wang et al., 2010)

A tsr^N96A heterozygous background enhances the patterning defects found in Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

(Johnson et al., 2008)

tsr^N96A/+ suppresses the mild rough eye phenotype seen mbt^P3 hemizygous males and largely suppresses the more severe rough eye phenotype seen in mbt^P1 hemizygous males.

(Menzel et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tsr^N96A is rescued by Scer\GAL4^ey-OK107/tsr^UAS.cNa

(Ng and Luo, 2004)

Partially rescued by

tsr^N96A is partially rescued by tsr^S3E.UAS/Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

tsr^N96A is partially rescued by Scer\GAL4^ey-OK107/tsr^S3A.UAS

(Ng and Luo, 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9108

y¹ w^*; P{FRT(w^hs)}G13 tsr^N96A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DmCFL^N96A

(Balakrishnan et al., 2020)

cofilin^N96A

(Abe et al., 2014)

tsr^N96A

(Sun et al., 2023, Balakrishnan et al., 2020, Sudarsanam et al., 2020, Skouloudaki et al., 2019, Nithianandam and Chien, 2018, Ko et al., 2016, Rudrapatna et al., 2014, Wang et al., 2010, Johnson et al., 2008, Menzel et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (12)

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