FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\twf110
Open Close
General Information
Symbol
Dmel\twf110
Species
D. melanogaster
Name
FlyBase ID
FBal0247644
Feature type
allele
Associated gene
Dmel\twf
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Wang et al., 2010)
Progenitor genotype
P{EP}twfEP3701
(Wang et al., 2010)
Cytology
Description

Imprecise excision of the progenitor insertion, P{EP}twfEP3701, removing most of the coding region of twf.

(Wang et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The majority of twf110 mutant animals die as larvae. 2.2% of animals survive to adulthood.

      The majority of twf110/twf102 mutant animals die as larvae. 2.2% of animals survive to adulthood.

      β lobes of the mushroom body cross the midline in approximately 50% of twf110 and 20% of twf110/Df(3R)su(Hw)7 mutant animal brains. In severe cases, the two β lobes are completely fused. β loves are bifurcated and thickened. 50% of twfEP3701 mutants display unilateral or bilateral loss of the adult mushroom body α lobe.

      β lobes of the mushroom body cross the midline in approximately 50% of twf110/twf102 mutant animal brains. In severe cases, the two β lobes are completely fused. β loves are bifurcated and thickened. 60% of twfEP3701 mutants display unilateral or bilateral loss of the adult mushroom body α lobe.

      At 29[o]C twfEP3701/twf110 mutant animals display a moderate but significant delay in border cell migration.

      twf110 mutant border cell clones display delayed migration at 25[o]C. 52% of clusters containing twf110 mutant clones display a migration delay, compared to 4% of control clones.

      twf110 animals show defects in endocytosis at the synaptic bouton, but no defects in exocytosis are observed. Treatment with a low concentration of latriculin A (0.1 μM) does not significantly effect endocytosis in twf110 mutants, compared to a significantly reduced level of endocytosis in treated controls. Treatment with a high concentration of latriculin A (10 μM) completely blocks endocytosis in both mutant and control animals.

      twf110 and twf110/Df(3R)su(Hw)7 mutants both show compromised neurotransmission under intense stimulation (10Hz to the nerve for 600 seconds). A decrease in postsynaptic excitatory junctional potentials (EJPs) is observed compared to controls. Basal transmission upon low levels of stimulation (0.3 Hz) is not affected in twf110 and twf110/Df(3R)su(Hw)7 mutants.

      The bristles of twf110 adult escapers show forked tips and severely twisted ridges. Bristle length is significantly shorter compared to controls.

      (Wang et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      twf110 has visible phenotype, suppressible | partially by chic[+]/chic221

      (Wang et al., 2010)
      Other
      Statement
      Reference

      tsr[+]/tsrN96A, twf110 has visible phenotype

      (Wang et al., 2010)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tsrN96A/+; twf110/+ transheterozygotes show mildly disorganised ridges and hooked tips.

      chic221/+ partially rescues the bristle phenotype of twf110 homozygous animals.

      (Wang et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      twf110 is rescued by twfUAS.cWa/Scer\GAL4Act.PU

      (Wang et al., 2010)
      Partially rescued by

      twf110 is partially rescued by twfUAS.cWa/Scer\GAL4C57

      (Wang et al., 2010)

      twf110 is partially rescued by twfUAS.cWa/Scer\GAL4elav.PU

      (Wang et al., 2010)
      Comments

      Expression of twfScer\UAS.cWa under the control of Scer\GAL4C57 partially rescues lethality of twf110 mutant animals. 22% of animals survive to adulthood.

      Expression of twfScer\UAS.cWa under the control of Scer\GAL4elav.PU partially rescues lethality of twf110 mutant animals. 70% of animals survive to adulthood.

      Expression of twfScer\UAS.cWa under the control of Scer\GAL4Act.PU rescues lethality of twf110 mutant animals. 100% of animals survive to adulthood.

      Pre-synaptic expression of twfScer\UAS.cWa under the control of Scer\GAL4elav.PU completely rescues the enhanced decrease in postsynaptic excitatory junctional potentials in twf110 mutant animals.

      (Wang et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      twf110
      (Ogienko et al., 2018, Wang et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)