FlyBase Allele Report: Dmel\puc[GS16811]

General Information

Symbol

Dmel\puc^GS16811

Species

D. melanogaster

Name

FlyBase ID

FBal0263488

Feature type

allele

Associated gene

Dmel\puc

Associated Insertion(s)

P{GSV6}puc^GS16811

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV6}puc^GS16811

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GSV6}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:8,119,863..8,119,863

(Okumura et al., 2010, Taniguchi et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval anterior midgut

(Taniguchi et al., 2007)

embryonic/larval anterior midgut (with Df(3R)dsx10D)

(Taniguchi et al., 2007)

embryonic/larval proventriculus

(Taniguchi et al., 2007)

Detailed Description

Statement

Reference

At stage 17, 37% of homozygous embryos show inversion of the normal left-right asymmetry of the anterior midgut, 14% do not show any left-right asymmetry and 49% show normal left-right asymmetry. In addition the valvular structure of the proventriculus fails to form and the embryos fail to complete dorsal closure.

No difference is detected between the tilting of the nuclei of the circular visceral muscle (CVM) cells on the right and left sides of the presumptive first midgut constriction in late stage 15 and 16 homozygous embryos. This contrasts with wild-type embryos at these stages, where the nuclei of a subset of CVM cells in the right ventral region of the presumptive first chamber of the midgut become tilted compared with those in the left-ventral region.

38% of stage 17 puc^GS16811/Df(3R)dsx10D embryos show inversion of the normal left-right asymmetry of the anterior midgut and 7% do not show any left-right asymmetry.

(Taniguchi et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

puc^GS16811 has embryonic/larval anterior midgut phenotype, suppressible | partially by bsk¹/bsk[+]

(Taniguchi et al., 2007)

puc^GS16811 has embryonic/larval anterior midgut phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^48Y

(Taniguchi et al., 2007)

puc^GS16811 has embryonic/larval anterior midgut phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^NP3392

(Taniguchi et al., 2007)

puc^GS16811 has embryonic/larval anterior midgut phenotype, suppressible by bsk^DN.UAS/bsk¹/bsk[+]/Scer\GAL4^NP3392

(Taniguchi et al., 2007)

Enhancer of

Statement

Reference

puc^GS16811/puc[+] is an enhancer of embryonic/larval anterior midgut phenotype of Rac1^V12.UAS, Scer\GAL4^NP3392

(Taniguchi et al., 2007)

NOT Enhancer of

Statement

Reference

puc^GS16811/puc[+] is a non-enhancer of embryonic/larval anterior midgut phenotype of Rho1^V14.UAS, Scer\GAL4^NP3392

(Taniguchi et al., 2007)

puc^GS16811/puc[+] is a non-enhancer of embryonic/larval anterior midgut phenotype of Cdc42^V12.UAS, Scer\GAL4^NP3392

(Taniguchi et al., 2007)

NOT Suppressor of

Statement

Reference

puc^GS16811/puc[+] is a non-suppressor of embryonic/larval anterior midgut phenotype of Rho1^V14.UAS, Scer\GAL4^NP3392

(Taniguchi et al., 2007)

puc^GS16811/puc[+] is a non-suppressor of embryonic/larval anterior midgut phenotype of Cdc42^V12.UAS, Scer\GAL4^NP3392

(Taniguchi et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

bsk¹/+ significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc^GS16811 embryos.

Expression of bsk^DN.Scer\UAS under the control of Scer\GAL4^48Y significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc^GS16811 embryos.

Expression of bsk^DN.Scer\UAS under the control of Scer\GAL4^Gap1-NP3392 significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous puc^GS16811 embryos. This suppression is stronger if the embryos are also carrying bsk¹/+.

puc^GS16811/+ significantly enhances the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Rac1^V12.Scer\UAS under the control of Scer\GAL4^Gap1-NP3392. 20% of the double mutant embryos show inversion of the normal left-right asymmetry and 42% show no left-right asymmetry.

puc^GS16811/+ has no effect on the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Rho1^V14.Scer\UAS under the control of Scer\GAL4^Gap1-NP3392.

puc^GS16811/+ has no effect on the defects in left-right asymmetry of the anterior midgut which are seen in embryos expressing Cdc42^V12.Scer\UAS under the control of Scer\GAL4^Gap1-NP3392.

(Taniguchi et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

puc^GS16811 is partially rescued by puc^UAS.cMa/Scer\GAL4^48Y

(Taniguchi et al., 2007)

puc^GS16811 is partially rescued by puc^UAS.cMa/Scer\GAL4^NP3392

(Taniguchi et al., 2007)

puc^GS16811 is partially rescued by puc^UAS.cMa/Scer\GAL4^hs.PU

(Taniguchi et al., 2007)

Not rescued by

puc^GS16811 is not rescued by puc^UAS.cMa/Scer\GAL4^dpp.PS

(Taniguchi et al., 2007)

puc^GS16811 is not rescued by puc^UAS.cMa/Scer\GAL4^NP7020

(Taniguchi et al., 2007)

Comments

Expression of puc^Scer\UAS.cMa under the control of either Scer\GAL4^48Y or Scer\GAL4^Gap1-NP3392 completely rescues the left-right asymmetry defects seen in the anterior midgut of puc^GS16811 embryos. The defects in dorsal closure are not rescued.

The lack of left-right asymmetry in the tilting of the nuclei of the circular visceral muscle which is seen in late stage 15 and 16 puc^GS16811 embryos is rescued by expression of puc^Scer\UAS.cMa under the control of Scer\GAL4^Gap1-NP3392.

Expression of puc^Scer\UAS.cMa under the control of either Scer\GAL4^dpp.PS or Scer\GAL4^NP7020 does not rescue the left-right asymmetry defects seen in the anterior midgut of puc^GS16811 embryos.

Expression of puc^Scer\UAS.cMa under the control of Scer\GAL4^hs.PU using heat shock for 30 minutes at 37[o]C significantly rescues the left-right asymmetry defects seen in the anterior midgut of puc^GS16811 embryos when the heat shock is applied at any time during 4.5 to 9.5 AEL.

(Taniguchi et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

206780

y¹ w^67c23; P{GSV6}puc^GS16811/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

puc^GS16811

(Okumura et al., 2010, Taniguchi et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (2)

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