fzy5032 larval brains show progressive neuroblast loss.
35% of fzy5032 mutant clones generated in the brain during the larval stages do not contain neuroblasts, compared to <10% in controls.
As in wild type, fzy5032 mutant neuroepithelial cell clones in the larval optic lobe proliferate to generate a large number of progeny.
Unlike in wild type, many fzy5032 mutant neuroblast clones in the larval brain fail to proliferate.
fzy5032/fzy4 mutant neuroblasts maintain a single nucleus and cell diameters are similar to wild type.
fzy5032/fzy1 larval brains show progressive neuroblast loss.
fzy5032/fzy1 mutant adults possess all appendages but have a dramatically reduced neuropil compared to controls. They exhibit poor motor function.
fzy5032/fzy1 mutant exhibit morphological and molecular marker changes consistent with aberrant onset of necrosis: in contrast to wild type, neuroblasts in fzy5032/fzy1 mutant brains often do not have identifiable nuclei, and their cytoplasm appears largely devoid of organelles and vacuoles. In these neuroblasts mitochondria are swollen and ruptured and remnants of the endoplasmic reticulum clump with remaining mitochondria into electron dense aggregates. Mitochondrial aggregates are seen in 25% of remaining fzy5032/fzy1 mutant neuroblasts. Elevated levels of reactive oxygen species and calcium ions are seen in fzy5032/fzy1 mutant neuroblasts compared to controls. Almost 40% of fzy5032/fzy1 mutant neuroblasts contain ubiquitin-conjugated protein aggregates, and these are virtually non-existent in controls.