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General Information
Symbol
Dmel\fzy5032
Species
D. melanogaster
Name
FlyBase ID
FBal0295946
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G16312117M

Amino acid change:

G291R | fzy-PA

Reported amino acid change:

G291R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G291R.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

fzy5032 larval brains show progressive neuroblast loss.

35% of fzy5032 mutant clones generated in the brain during the larval stages do not contain neuroblasts, compared to <10% in controls.

As in wild type, fzy5032 mutant neuroepithelial cell clones in the larval optic lobe proliferate to generate a large number of progeny.

Unlike in wild type, many fzy5032 mutant neuroblast clones in the larval brain fail to proliferate.

fzy5032/fzy4 mutant neuroblasts maintain a single nucleus and cell diameters are similar to wild type.

fzy5032/fzy1 larval brains show progressive neuroblast loss.

fzy5032/fzy1 mutant adults possess all appendages but have a dramatically reduced neuropil compared to controls. They exhibit poor motor function.

fzy5032/fzy1 mutant exhibit morphological and molecular marker changes consistent with aberrant onset of necrosis: in contrast to wild type, neuroblasts in fzy5032/fzy1 mutant brains often do not have identifiable nuclei, and their cytoplasm appears largely devoid of organelles and vacuoles. In these neuroblasts mitochondria are swollen and ruptured and remnants of the endoplasmic reticulum clump with remaining mitochondria into electron dense aggregates. Mitochondrial aggregates are seen in 25% of remaining fzy5032/fzy1 mutant neuroblasts. Elevated levels of reactive oxygen species and calcium ions are seen in fzy5032/fzy1 mutant neuroblasts compared to controls. Almost 40% of fzy5032/fzy1 mutant neuroblasts contain ubiquitin-conjugated protein aggregates, and these are virtually non-existent in controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Dronc51

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Atg7d77

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Dark82

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by DreddB118

fzy1/fzy5032 has neuroblast | larval stage phenotype, non-suppressible by Atg125/Atg13

Additional Comments
Genetic Interactions
Statement
Reference

Dronc51 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Dark82 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

DreddB118 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Atg125/Atg13 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Atg7d77 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

AIFT52 partially suppresses the larval neuroblast proliferation defects seen in fzy5032 mutant clones: the double mutant clones consistently contain more progeny than a fzy5032 single mutant clone, albeit the frequency of neuroblast-containing clones appears similar in both single and double mutant clones.

bsk1 partially suppresses the larval neuroblast proliferation defects seen in fzy5032 mutant clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

Expression of fzytDa rescues the neuroblast loss seen in fzy5032 mutant larval brains.

Expression of fzytDa rescues the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)