Flies homozygous for Pknk06808 are unable to fly and their wings are held up from the thorax and curve ventrally, similar to the open doors of a Delorean automobile. The extent of curvature is variable, from slight smooth curve to severe folded-over phenotype. The anterior wing margin is also defective: there are gaps in marginal tissue, excessive spacing between stout mechanosensory bristles, and fewer sensillae and bristles relative to heterozygous siblings. Occasionally, two or three stout bristles share a common socket, and sometimes a stout bristle appears in the margin tissue without a socket. A total of 95% of homozygous Pknk06808 flies analysed have one or more double-stout bristle clusters, and 15% have triple-stout bristle clusters.
Wing defects are only seen in homozyogus Pknk06808 flies. Heterozygous Pknk06808/+ flies have wild-type wings.
Wing vein defects, such as ectopic vein material surrounding the longitudinal vein L2, and/or extending from the posterior crossvein, are also noted.
Pknk06808/Pknk06808; Tp(2;3)eve1.18 wings do not exhibit ventral curvature and flies no longer have ante- rior wing margin bristle defects or ectopic veins associated with the delorean phenotype.
When homozygous Pknk06808 offspring are compared with heterozygous Pknk06808/+ siblings, no significant difference in viability is observed. Thus, the Pknk06808 allele is adult viable.
Unexpectedly, wing shape, anterior wing margin structures, and venation patterns of Pknk06808/Df(2R)w45-30n flies are mostly wild-type. This was seen for all deficiencies of 45C examined (Df(2R)Np3, Df(2R)Np5, and Df(2R)wun-GL).
Homozygotes have wings that stand up from the thorax and curve down. The phenotype also manifests itself in mechanosensory bristles of the anterior wing margins.