FlyBase Allele Report: Dmel\raw[155.27]

General Information

Symbol

Dmel\raw^155.27

Species

D. melanogaster

Name

FlyBase ID

FBal0260785

Feature type

allele

Associated gene

Dmel\raw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Weyers et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: R949C.

(Hao et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,710,560..8,710,560 [-]

Nucleotide change:

C8710560T

Amino acid change:

Reported amino acid change:

R949C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Hao et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage | conditional (with raw^dcp-1)

(Hao et al., 2019)

Phenotype Manifest In

axon | third instar larval stage | conditional (with raw^dcp-1)

(Hao et al., 2019)

embryonic/larval motor neuron | third instar larval stage | conditional (with raw^dcp-1)

(Hao et al., 2019)

germline cell | embryonic stage 15 (with raw^134.47)

(Jemc et al., 2012)

germline cell | embryonic stage (with raw^134.47)

(Weyers et al., 2011)

gonad | embryonic stage (with raw^134.47)

(Weyers et al., 2011)

gonadal sheath proper primordium | embryonic stage (with raw^134.47)

(Weyers et al., 2011)

male germline cell | embryonic stage (with raw^134.47)

(Jemc et al., 2012)

Detailed Description

Statement

Reference

raw^dcp-1/raw^155.27 transheterozygozity provides protection against (injury-induced) Wallerian degeneration of third instar larval motor neurons.

(Hao et al., 2019)

While only 5% of gonads exhibit defects in germ cell ensheathment in heterozygous controls, raw^134.47/raw^155.27 mutants exhibit ensheathment defects in 45% of gonads. Mutant germ cells appear to form clumps surrounded by somatic gonadal precursor cells at the periphery of the gonad in stage 15 developing embryos.

(Jemc et al., 2012)

raw^134.47/raw^155.27 embryos have gonads in which somatic gonadal precursors (SGPs) fail to wrap individual germ cells. Consequently, the unensheathed germ cells are associated in larger clusters rather than being separated by the SGPs. Somatic gonadal precursor specification appears normal. Visceral mesoderm appears normal.

(Weyers et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

raw^155.27/raw[+] is a suppressor | partially of increased cell number | oogenesis phenotype of Scer\GAL4^C587, Su(var)3-3^NIG.17149R

(Eliazer et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, enhanceable by shg[+]/shg^g317

(Jemc et al., 2012)

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, enhanceable by shg^g317

(Jemc et al., 2012)

Suppressed by

Statement

Reference

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^αTub84B.PL

(Jemc et al., 2012)

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, suppressible by Jra^IA109/Jra[+]

(Jemc et al., 2012)

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, suppressible by bsk¹/bsk[+]

(Jemc et al., 2012)

raw^155.27/raw^134.47 has germline cell | embryonic stage 15 phenotype, suppressible by shg^αTub84B.PP

(Jemc et al., 2012)

Suppressor of

Statement

Reference

raw^155.27/raw[+] is a suppressor | partially of egg chamber | decreased number phenotype of Scer\GAL4^C587, Su(var)3-3^NIG.17149R

(Eliazer et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^αTub84B.PP (presumably in the soma) is sufficient to strongly suppress the ensheathment defects in raw^134.47/raw^155.27 mutant gonads.

shg^g317/+ enhances the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants. Homozygous shg^g317 does so even at a greater frequency.

bsk¹/+ suppresses the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants.

Jra^IA109/+ suppresses the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants.

Expression of bsk^DN.Scer\UAS using Scer\GAL4^αTub84B.PL suppresses the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants.

(Jemc et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

raw(ensh)^155.27

(Weyers et al., 2011)

raw^155.27

(Rui et al., 2020, Hao et al., 2019, Eliazer et al., 2014, Jemc et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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