FlyBase Allele Report: Dmel\shg[αTub84B.PP]

General Information

Symbol

Dmel\shg^αTub84B.PP

Species

D. melanogaster

Name

α-Tubulin at 84B promoter construct of Pacquelet

FlyBase ID

FBal0154611

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{tub-shg.P}

Key Links

Transgenic product class

wild_type

(Pacquelet et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pacquelet et al., 2003)

Mutagen

in vitro construct

(Pacquelet et al., 2003)

Progenitor genotype

Carried in construct

P{tub-shg.P}

Cytology

Description

A αTub84B promoter drives expression of shg.

(Pacquelet et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos that express shg^αTub84B.PP show no gross morphological defects.

(Mathews et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

shg^αTub84B.PP is a non-suppressor of abnormal cell shape | somatic clone | cell autonomous phenotype of ed^1X5

(Chang et al., 2011)

shg^αTub84B.PP is a non-suppressor of abnormal cell shape | somatic clone | cell non-autonomous phenotype of ed^1X5

(Chang et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

shg^αTub84B.PP is a suppressor of germline cell | embryonic stage 15 phenotype of raw^155.27/raw^134.47

(Jemc et al., 2012)

shg^αTub84B.PP is a suppressor of gonad | embryonic stage phenotype of foi^20.71

(Mathews et al., 2006)

NOT Suppressor of

Statement

Reference

shg^αTub84B.PP is a non-suppressor of wing disc | somatic clone phenotype of ed^1X5

(Chang et al., 2011)

shg^αTub84B.PP is a non-suppressor of wing disc | somatic clone | cell autonomous phenotype of ed^1X5

(Chang et al., 2011)

shg^αTub84B.PP is a non-suppressor of wing disc | somatic clone | cell non-autonomous phenotype of ed^1X5

(Chang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^αTub84B.PP (presumably in the soma) is sufficient to strongly suppress the ensheathment defects in raw^134.47/raw^155.27 mutant gonads.

(Jemc et al., 2012)

Expression of shg^αTub84B.PP is unable to rescue the cell sorting phenotypes seen in ed^1X5 mutant wing disc clones.

(Chang et al., 2011)

Expression of shg^αTub84B.PP fully rescues the gonadal phenotype of foi^20.71 mutants in 22% of cases.

(Mathews et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

shg^αTub84B.PP partially rescues shg^R69

(Mathews et al., 2006)

shg^αTub84B.PP partially rescues shg¹

(Pacquelet et al., 2003)

shg^αTub84B.PP partially rescues shg^R69

(Pacquelet et al., 2003)

Comments

Expression of the shg^αTub84B.PP transgene largely rescues the gonadal coalescence defects of shg^R69 embryos.

(Mathews et al., 2006)

Expression of shg^αTub84B.PP in shg¹ mutant border cells or nurse cells rescues the border cell migration phenotype seen in these cells. Expression of shg^αTub84B.PP rescues the head and ventral epidermis phenotypes seen in homozygous shg¹ embryos. Expression of shg^αTub84B.PP rescues the head and ventral epidermis phenotypes seen in homozygous shg¹ embryos. 10-25% of homozygous shg¹ carrying shg^αTub84B.PP hatch, and the resulting larvae look normal. When shg^αTub84B.PP is expressed in shg^R69 homozygotes a few percent of the mutants are rescued to adulthood.

(Pacquelet et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shg^αTub84B.PP

Name Synonyms

α-Tubulin at 84B promoter construct of Pacquelet

(Pacquelet et al., 2003)

Secondary FlyBase IDs

References (5)

Report Sections

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