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General Information
Symbol
Dmel\knUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Mohler
FlyBase ID
FBal0103782
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-kn
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the entire kn open reading frame.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of knScer\UAS.cMa under the control of Scer\GAL4GMR.PU results in severely disrupted ommatidial patterning in the pupal eye.

Overexpression of knScer\UAS.cMa in class I da neurons (using MARCM and Scer\GAL421-7) results in a significant increase in total dendrite length.

Ectopic expression of knScer\UAS.cMa (under the control of Scer\GAL4221) significantly increases the number of dendrite termini by 19%, total arbor length by 46%, and arbor area by 18%. In class I neurons expressing knScer\UAS.cMa, the extra length of dendrite is preferentially concentrated in the last segments of the dendritic arbor.

Ectopic expression of knScer\UAS.cMa (under the control of Scer\GAL4smid-C161) in class II neurons results in a large increase in dendritic arbor complexity in third instar larvae. In the same way, ectopic knScer\UAS.cMa expression increases class III neuron (v'pda) total arbor length by 19%. The number of filopodia/spikes, defined as very thin and short lateral projections from the main dendrite trunk, is highly reduced.

Ectopic expression of knScer\UAS.cMa (under the control of Scer\GAL4109(2)80) in class IV neurons results in a strong reduction in the number of filopodia/spikes in a 75υm radius around the cell body to 23% of wild-type levels.

Expression of knScer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 results in wings lacking vein 3, the distal portion of vein 4 and the proximal portion of vein 5. Vein 2, the proximal part of vein 4 and the distal part of vein 5 are diffuse, wide and plexate. Expression of knScer\UAS.cMa under the control of Scer\GAL4dpp.blk1 often results in the disruption of wing vein 3; the distal portion is disrupted and shifted anteriorly, meeting the wing margin considerably anterior to the posterior-most socketed bristle (in contrast to wild type, where most socketed bristles are anterior to vein 3). The distance from vein 4 to the posterior-most socketed bristle is unchanged from wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

A dar13010 mutant background suppresses the increase in dendrite length found upon expression of knScer\UAS.cMa in class I da neurons under the control of Scer\GAL421-7 and results in a severe reduction in dendrite length.

Co-expression of knScer\UAS.cMa in a Scer\GAL4bi-md653, A2bp1dsRNA.Scer\UAS background rescues the loss of intervein tissue phenotype and causes a loss of vein tissue.

Ectopic expression of Rac1Scer\UAS.cLa along with knScer\UAS.cMa in class I neurons under the control of Scer\GAL4221 promotes the outgrowth of bona fide dendrites from the thorn-shaped projections initiated by Rac1Scer\UAS.cLa expression. In addition, coexpression of knScer\UAS.cMa causes a small, significant reduction in termini number.

Co-expression of knScer\UAS.cMa and ctScer\UAS.cPa under the control of Scer\GAL4221 increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%.

Ectopic expression of spasdsRNA.Scer\UAS strongly reduces both branching and total dendrite length by 18 and 19% respectively, when expressed in knScer\UAS.cMa-expressing class I neurons (both transgenes under the control of Scer\GAL4221).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
knScer\UAS.cMa
knUAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Mohler
Secondary FlyBase IDs
    References (8)