A dar13010 mutant background suppresses the increase in dendrite length found upon expression of knScer\UAS.cMa in class I da neurons under the control of Scer\GAL421-7 and results in a severe reduction in dendrite length.
Ectopic expression of Rac1Scer\UAS.cLa along with knScer\UAS.cMa in class I neurons under the control of Scer\GAL4221 promotes the outgrowth of bona fide dendrites from the thorn-shaped projections initiated by Rac1Scer\UAS.cLa expression. In addition, coexpression of knScer\UAS.cMa causes a small, significant reduction in termini number.
Co-expression of knScer\UAS.cMa and ctScer\UAS.cPa under the control of Scer\GAL4221 increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%.
Ectopic expression of spasdsRNA.Scer\UAS strongly reduces both branching and total dendrite length by 18 and 19% respectively, when expressed in knScer\UAS.cMa-expressing class I neurons (both transgenes under the control of Scer\GAL4221).