FlyBase Allele Report: Dmel\peb[EP55]

General Information

Symbol

Dmel\peb^EP55

Species

D. melanogaster

Name

FlyBase ID

FBal0057934

Feature type

allele

Associated gene

Dmel\peb

Associated Insertion(s)

P{EP}peb^EP55

Carried in Construct

Also Known As

EP55, hnt^EP55

Key Links

Genomic Maps

JBrowse

Allele class

wild-type allele

Mutagen

P-element activity

Nature of the Allele

Allele class

wild-type allele

(Rorth, 1996)

Mutagen

P-element activity

(Rorth, 1996)

Progenitor genotype

Associated Insertion(s)

P{EP}peb^EP55

Cytology

Description

The P{EP} insertion is located 585bp upstream of the peb cDNA NB701, with the 3' of the element closest to the peb exon.

(Reed et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:4,617,262..4,617,262 [+]

(Ermanoska et al., 2023, Oramas et al., 2023, Knapp et al., 2019, Deady et al., 2017, Krejcí et al., 2009, Bilen et al., 2006, Pallavi et al., 2006, Reed et al., 2001, Rorth, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oviposition, with Scer\GAL4^GMR44E10

(Deady et al., 2017)

lethal, with Scer\GAL4^32B

(Rorth, 1996)

lethal, with Scer\GAL4^h-1J3

(Rorth, 1996)

lethal, with Scer\GAL4^how-24B

(Rorth, 1996)

lethal, with Scer\GAL4^hs.2sev

(Rorth, 1996)

lethal, with Scer\GAL4^T80

(Rorth, 1996)

partially lethal - majority die, with Scer\GAL4^69B

(Rorth, 1996)

visible, with Scer\GAL4^69B

(Rorth, 1996)

Phenotype Manifest In

egg chamber | oogenesis stage S14, with Scer\GAL4^GMR44E10

(Deady et al., 2017)

egg chamber | oogenesis stage S14, with Scer\GAL4^GMR47A04

(Deady et al., 2017)

eye, with Scer\GAL4^69B

(Rorth, 1996)

oocyte associated follicular epithelium | oogenesis stage S14, with Scer\GAL4^GMR44E10

(Deady et al., 2017)

oocyte associated follicular epithelium | oogenesis stage S14, with Scer\GAL4^GMR47A04

(Deady et al., 2017)

Detailed Description

Statement

Reference

Females expressing peb^EP55 under the control of Scer\GAL4^GMR44E10 do not show significant egg laying defects (i.e. number of eggs laid and mature follicles accumulated in the ovary); their mature follicles rupture as frequently upon induction by OA or ionomycin, as compared to controls.

(Knapp et al., 2019)

The overexpression of peb^EP55 under the control of Scer\GAL4^GMR44E10 leads to a significant increase in egg chamber follicle rupture, whereas under the control of Scer\GAL4^GMR47A04 leads to a significant decrease in egg chamber follicle rupture, as compared to controls (ex vivo observations in octopamine-treated egg chambers). Expression under the control of Scer\GAL4^GMR44E10, but not of Scer\GAL4^GMR47A04, leads to a small but significant decrease in the number of eggs laid.

(Deady et al., 2017)

Flies have black spots on the eyes when peb^EP55 expression is driven by Scer\GAL4^69B.

(Rorth, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

peb^EP55/Scer\GAL4^GMR44E10 is a suppressor | partially of abnormal oviposition phenotype of Scer\GAL4^GMR44E10, ct^UAS.cPa