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General Information
Symbol
Dmel\pbUAS.A
Species
D. melanogaster
Name
FlyBase ID
FBal0059978
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-pb
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full-length pb cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When pbScer\UAS.A is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

Expression of pbScer\UAS.A under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

When pbScer\UAS.A is driven by Scer\GAL4dpp.blk1 a substantial eye loss phenotype as well as wing and leg defects are seen. When pbScer\UAS.A is driven by Scer\GAL4ey.PH a substantial or full eye loss phenotype is seen. When driven by Scer\GAL4GMR.PF no effect is seen.

Most imaginal discs expressing pbScer\UAS.A under the control of Scer\GAL4dpp.blk1 are misshapen; the discs have abnormal folding and do not lay flat like wild-type discs.

Expression of pbScer\UAS.A under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

Scer\GAL4dpp.blk1-mediated expression in the imaginal discs results in striking growth and patterning defects in the antenna, legs and wings. Upon eclosion flies are unable to pull themselves free of the pupal case. Labial-like structures are observed along the T1 legs, maxillary palp-like structures are formed along the lateral aspects of the legs in some locations. General leg patterning defects include a shortening and distortion of the leg along the PD axis, fusion of tarsal segments and large bulbous outgrowths of tissue along the lateral surfaces. Wings do not exhibit homeotic transformation but are smaller in size than wild type, are missing both longitudinal and cross veins, have ectopic socketed bristles growing from the wing blade surface and display a crumpled appearance. Ectopic expression does not increase apoptosis in the wing, leg, haltere or eye discs. Scer\GAL469B- or Scer\GAL471B-mediated expression causes embryonic or early larval lethality. Scer\GAL430A-mediated expression causes minor non-homeotic disruptions of the legs that include fusion of tarsal segments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of pbScer\UAS.A strongly suppresses the disorganised eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4.

When pbScer\UAS.A is overexpressed along with ciScer\UAS.cAa, both under the control of Scer\GAL4pb.PJ, the ciScer\UAS.cAa phenotype is suppressed and labial palps appear almost the same as wild type.

Expression of pbScer\UAS.A under the control of Scer\GAL4lab.PH rescues the tritocerebral defects seen in lab14 embryonic brains. 41.7% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab14 phenotype is 88.6%).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pbScer\UAS.A
pbUAS.A
Name Synonyms
Secondary FlyBase IDs
    References (10)