Expression of pbScer\UAS.A under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.
Most imaginal discs expressing pbScer\UAS.A under the control of Scer\GAL4dpp.blk1 are misshapen; the discs have abnormal folding and do not lay flat like wild-type discs.
Expression of pbScer\UAS.A under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.
Scer\GAL4dpp.blk1-mediated expression in the imaginal discs results in striking growth and patterning defects in the antenna, legs and wings. Upon eclosion flies are unable to pull themselves free of the pupal case. Labial-like structures are observed along the T1 legs, maxillary palp-like structures are formed along the lateral aspects of the legs in some locations. General leg patterning defects include a shortening and distortion of the leg along the PD axis, fusion of tarsal segments and large bulbous outgrowths of tissue along the lateral surfaces. Wings do not exhibit homeotic transformation but are smaller in size than wild type, are missing both longitudinal and cross veins, have ectopic socketed bristles growing from the wing blade surface and display a crumpled appearance. Ectopic expression does not increase apoptosis in the wing, leg, haltere or eye discs. Scer\GAL469B- or Scer\GAL471B-mediated expression causes embryonic or early larval lethality. Scer\GAL430A-mediated expression causes minor non-homeotic disruptions of the legs that include fusion of tarsal segments.