FlyBase Allele Report: Dmel\ebi[k16213]

(Marygold et al., 2011, Tsuda et al., 2002, Boulton et al., 2000, Dong et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensory system disease

CEA

(Lim et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

ameliorates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive | somatic clone

(Lim et al., 2012)

abnormal size (with ebi^P7)

(Marygold et al., 2011)

lethal (with ebi^2g1)

(Marygold et al., 2011)

lethal (with ebi^2g2)

(Marygold et al., 2011)

lethal | recessive

(Marygold et al., 2011, Boulton et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

partially lethal - majority die (with ebi^P7)

(Marygold et al., 2011)

Phenotype Manifest In

embryonic/larval crystal cell

(Milchanowski et al., 2004)

eye | somatic clone

(Lim et al., 2012)

mitotic cell cycle

(Milchanowski et al., 2004)

retina | progressive | somatic clone | conditional

(Lim et al., 2012)

wing (with ebi^P7)

(Marygold et al., 2011)

Detailed Description

Statement

Reference

One week after eclosion, homozygous ebi^k16213 eye clones form ommatidia with a near-normal set of photoreceptors. At 5 weeks, however, these cells show a severely degenerated phenotype in a cell-autonomous manner. Several scar-like defects appear in the retinas along with abnormal ommatidia with a reduced number of photoreceptor cells.

Eyes containing large ebi^k16213 clones have a mildly rough phenotype.

Flies with retinae containing large ebi^k16213 clones cultured under constant illumination show severe degeneration by 14 days after eclosion, whereas wild- type ommatidia do not show any structural changes under the same conditions. In contrast, when mutant flies are raised under constant darkness, the eye phenotype remains mild 4 weeks after eclosion.

(Lim et al., 2012)

ebi^P7/ebi^k16213 wings are 82% the size of control wings. Gross defects in wing patterning are not apparent. There is no significant decrease in the size of other organs or the whole organism.

(Marygold et al., 2011)

The average number of crystal cells per embryo is reduced (to 13.6) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). Mutant stage 14 embryos show cell cycle defects in the head region.

(Milchanowski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ebi[+]/ebi^k16213 is an enhancer of abnormal size phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Marygold et al., 2011)

NOT Enhancer of

Statement

Reference

ebi[+]/ebi^k16213 is a non-enhancer of abnormal size phenotype of Scer\GAL4^Bx-MS1096, aos^UAS.cMa

(Marygold et al., 2011)

ebi[+]/ebi^k16213 is a non-enhancer of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Suppressor of

Statement

Reference

ebi[+]/ebi^k16213 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

ebi[+]/ebi^k16213 is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

ebi[+]/ebi^k16213 is a suppressor of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

ebi[+]/ebi^k16213 is a non-suppressor of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Other

Statement

Reference

ebi[+]/ebi^k16213, sinah¹ has visible phenotype

(Cooper, 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

ebi^k16213 has eye | somatic clone phenotype, enhanceable by puc^E69/puc[+]

(Lim et al., 2012)

Suppressed by

Statement

Reference

ebi^k16213 has eye | somatic clone phenotype, suppressible by hep[+]/hep^r75

(Lim et al., 2012)

Enhancer of

Statement

Reference

ebi[+]/ebi^k16213 is an enhancer of wing margin phenotype of sd^ETX4

(Marygold et al., 2011)

ebi[+]/ebi^k16213 is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Marygold et al., 2011)

ebi^k16213 is an enhancer of wing margin phenotype of sno^71e3

(Tsuda et al., 2002)

ebi^k16213 is an enhancer of eye phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Boulton et al., 2000)

ebi^k16213 is an enhancer of interommatidial bristle phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Boulton et al., 2000)

ebi^k16213 is an enhancer of ommatidium phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Boulton et al., 2000)

NOT Enhancer of

Statement

Reference

ebi[+]/ebi^k16213 is a non-enhancer of wing vein phenotype of Scer\GAL4^Bx-MS1096, aos^UAS.cMa

(Marygold et al., 2011)

ebi[+]/ebi^k16213 is a non-enhancer of eye phenotype of Egfr^E1

(Read et al., 2005)

Suppressor of

Statement

Reference

ebi[+]/ebi^k16213 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

ebi[+]/ebi^k16213 is a suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

ebi[+]/ebi^k16213 is a suppressor of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

ebi[+]/ebi^k16213 is a non-suppressor of eye phenotype of Egfr^E1

(Read et al., 2005)

Other

Statement

Reference

ebi[+]/ebi^k16213, sinah¹ has dorsocentral bristle | ectopic phenotype

(Cooper, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

hep^r75/+ suppresses while puc^E69/+ enhances the mild rough eye phenotype seen in eyes containing large ebi^k16213 clones.

(Lim et al., 2012)

Heterozygosity for ebi^k16213 enhances the small wing phenotype induced by Scer\GAL4^Bx-MS1096-mediated expression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}.

Heterozygosity for ebi^k16213 enhances the notched wing margin phenotype seen in sd^ETX4 hemizygous males.

(Marygold et al., 2011)

ebi^k16213/+ ; sinah¹/sinah¹ flies have an extra dorsocentral bristle; on average flies contain 0.82 extra bristles and one or more extra bristles are seen in 36% of males and 68% of females. The dorsocentral bristles are aligned and spaced in an anterior to posterior row as in wild type, but because of the extra bristle(s), the row extends further anteriorly than in wild type.

(Cooper, 2007)

Enhances the rough eye phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH, increasing the irregular arrangement of the ommatidial facets. A large number of inter-ommatidial bristle duplications are also seen.

(Boulton et al., 2000)

Xenogenetic Interactions

Statement

Reference

(Boulton et al., 2000)

Complementation and Rescue Data

Fails to complement

ebi^CC1

(Boulton et al., 2000)

ebi^CC3

(Boulton et al., 2000)

ebi^CC4

(Boulton et al., 2000)

Rescued by

ebi^k16213 is rescued by ebi^+t7.5

(Marygold et al., 2011)

Comments

ebi^+t7.5 rescues the homozygous lethality of ebi^k16213.

(Marygold et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

11166

y¹ w^67c23; P{lacW}ebi^k16213/CyO

Kyoto

108864

y¹ w^67c23; P{lacW}ebi^k16213/CyO

111356

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}ebi^k16213 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Separable from: A second site lethal mutation.

(Marygold et al., 2011)

The P{lacW}ebi^k16213 chromosome contains at least two lethal mutations: ebi^k16213, and a second site lethal mutation that can be removed by recombination.

(Marygold et al., 2011)

Comments

Excision of the P{lacW} element reverts the lethality associated with this allele.

(Boulton et al., 2000)

Complements: ex⁰¹²⁷⁰. Complements: lwr^01519a. Complements: ex⁰²²⁷⁸. Complements: ds⁰⁵¹⁴². Complements: α-Adaptin⁰⁶⁶⁹⁴. Complements: ex^k01115. Complements: RpI135^k16513.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Ebi^k16213

(Cooper, 2007)

P11166

(Milchanowski et al., 2004)

ebi^K16213

(Boulton et al., 2000)

ebi^P1166

(Tsuda et al., 2002)

ebi^P

(Lim et al., 2012, Dong et al., 1999)

ebi^k16213

(Nguyen et al., 2016, Marygold et al., 2011)

l(2)k16213

(Spradling et al., 1999)

l(2)k16213^k16213

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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