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General Information
Symbol
Dmel\DJ-1αdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0190191
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DJ-1A RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of genomic DJ-1α sequence fused with an inverted DJ-1α cDNA fragment corresponding to the same region to generate dsRNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of DJ-1αdsRNA.Scer\UAS under the control of Scer\GAL4Ddc.PL results in accelerated age-dependent loss of dopaminergic neurons in adult flies (reared on a yeast-paste diet) aged flies also display decreased climbing ability relative to controls.

Flies expressing DJ-1αdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF at 29[o]C have black lesions on the eye surface.

Induction of DJ-1αdsRNA.Scer\UAS in the developing eye using Scer\GAL4GMR.PF produces a rough eye phenotype, caused through a loss of photoreceptor neurons and some ommatidia. This RNAi effect is dosage dependent as increasing the copy number of Scer\GAL4GMR.PF and DJ-1αdsRNA.Scer\UAS results in a more severe degeneration of the eye. In a Df(2R)CX1 heterozygous background (Df(2R)CX1 removes DJ-1α), the eye phenotype of DJ-1αdsRNA.Scer\UAS/Scer\GAL4GMR.PF is significantly enhanced. Expression of DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4Ddc.PL results in an age-dependent reduction in the number of ple-positive (dopaminergic) neurons in the dorsomedial clusters. In 1-day-old flies, a normal complement of ple positive neurons (approximately 18) is present, but, in 35-day and old flies, only 10-12 of these neurons can be detected. Induction of DJ-1αdsRNA.Scer\UAS with Scer\GAL4ple.PF or Scer\GAL4elav-C155 results in a reduction in ple-positive dopaminergic neurons in the dorsomedial clusters. In newly eclosed flies the numbers of dopaminergic neurons is comparable between control and DJ-1αdsRNA.Scer\UAS (Scer\GAL4ple.PF) flies. However, 1 day after eclosion, DJ-1αdsRNA.Scer\UAS flies exhibit significantly reduced dopaminergic neuron levels compared to wild-type. At 4,7 and 10 dyas of age, control flies and DJ-1αdsRNA.Scer\UAS flies exhibit an age-dependent decline in dopaminergic neurons, although DJ-1αdsRNA.Scer\UAS flies consistently exhibit more reduction than in controls. When treated with 1% H2O2, the time to reach 50% mortality is reduced by 27% in DJ-1αdsRNA.Scer\UAS (under the control of Scer\GAL4elav-C155) compared to controls. DJ-1αdsRNA.Scer\UAS (under the control of Scer\GAL4elav-C155) flies are more sensitive to 3-AT (a known inhibitor of catalase, which converts H2O2 to H20) exposure compared to control flies.

Induction of DJ-1αdsRNA.Scer\UAS in aduly fly dopaminergic neurons (through Scer\GAL4ple.PF leads to an elevation in the levels of reactive oxygen species (ROS).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

Coexpression of PtenScer\UAS.cHa with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF enhances the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. The resulting eyes are dramatically reduced in size, with collapsed and fused ommatidia and necrotic spots, which are not present in DJ-1αdsRNA.Scer\UAS flies. There is also a near complete loss of photoreceptor neurons. Coexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF enhances the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. Coexpression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4Ddc.PL results in a statistically significant enhancement of the dopaminergic (DA) degeneration phenotype of DJ-1αdsRNA.Scer\UAS mutants. Coexpression of Pi3K92EScer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF suppresses the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. The eyes are restored to normal size, and the organisation of the ommatidia is significantly improved. Coexpression of Pi3K92EScer\UAS.T:Hsap\MYC with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4Ddc.PL completely suppresses the dopaminergic (DA) degeneration phenotype of DJ-1αdsRNA.Scer\UAS mutants. The number of DA neurons remains consistent throughout. Coexpression of Akt1Scer\UAS.T:Ivir\HA1 with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF suppresses the DJ-1αdsRNA.Scer\UAS-induced eye phenotype.

DJ-1αdsRNA.Scer\UAS flies coexpressing Pi3K92EScer\UAS.T:Hsap\MYC (both in the dopaminergic neurons through Scer\GAL4ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type. DJ-1αdsRNA.Scer\UAS flies coexpressing PtenScer\UAS.cHa (both in the dopaminergic neurons through Scer\GAL4ple.PF) exhibit higher levels of reactive oxygen species (ROS), compared to flies expressing DJ-1αdsRNA.Scer\UAS alone.

Xenogenetic Interactions
Statement
Reference

The accelerated age-dependent loss of dopaminergic neurons as well as the decrease in climbing ability in aged adults characteristic for flies expressing DJ-1αdsRNA.Scer\UAS under the control of Scer\GAL4Ddc.PL (reared on a yeast-paste diet) is rescued by co-expression of Hsap\PARK7Scer\UAS.cYa.

Co-expression of Hsap\DJ-1AScer\UAS.cYa with DJ-1αdsRNA.Scer\UAS, under the regulation of Scer\GAL4GMR.PF partially suppresses the eye degeneration phenotype observed in DJ-1αdsRNA.Scer\UAS (Scer\GAL4GMR.PF) mutants. Co-expression of Avic\GFPScer\UAS with DJ-1αdsRNA.Scer\UAS, under the regulation of Scer\GAL4GMR.PF has no effect on the DJ-1αdsRNA.Scer\UAS eye degeneration phenotype.

Complementation and Rescue Data
Partially rescued by
Comments

Expression of a DJ-1αScer\UAS.cYa transgene partially rescues the DJ-1αdsRNA.Scer\UAS (Scer\GAL4GMR.PF) eye degeneration phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DJ-1αdsRNA.Scer\UAS
DJ-1αdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (6)