Neuronal expression of PtenScer\UAS.cGb under the control of Scer\GAL4elav-C155 decreases sensitivity to ethanol sedation.
Neuronal expression of PtenScer\UAS.cGb (under the control of Scer\GAL4elav-C155) throughout development (until eclosion of the adult fly, when Scer\GAL80ts.αTub84B is induced) results in reduced ethanol sensitivity. The converse experiment, with over-expression in neurons only after eclosion, does not alter ethanol sensitivity.
Expression of PtenScer\UAS.cGb in the prothoracic gland and corpora allata, driven by Scer\GAL4P0206, suppresses the growth of both of these ring gland components. Scer\GAL4P0206>PtenScer\UAS.cGb flies have larger bodies than controls; this phenotype is first seen at the L2 stage, as evidenced by the length of the mouth hooks. As pharate adults, these flies are 42% heavier than controls. This phenotype continues into adulthood as can be seen by the unusually large adult wing areas. Scer\GAL4P0206>PtenScer\UAS.cGb larvae are developmentally delayed and molt to L2 later than controls. These larvae either molt to L3 later than controls or pupariate prematurely in the L2 stage. None of the L2 puparia eclose, although a few (<1%) develop to the pharate adult stage. Rearing Scer\GAL4P0206>PtenScer\UAS.cGb larvae on yeast paste enhances the amount of precocious L2 puparia, while rearing larvae on food containing 50% of the normal nutrients suppresses this phenotype, resulting in all larvae forming large L3 puparia. Expression of PtenScer\UAS.cGb in the prothoracic gland, driven by Scer\GAL4phm.PO, causes a prolonged L1 stage followed by death. Expression of PtenScer\UAS.cGb in the corpora allata, driven by Scer\GAL4Aug21, has no effect on pharate adult weight or on adult wing area.
When fed ad libitum, third instar larvae expressing PtenScer\UAS.cGb, under the control of Scer\GAL4NPFR1.6.6 display hyperactive feeding on solid food, similar to control third instar larvae deprived of food for 40 minutes. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.
When expression is driven in clones in the early L3 larval fat body increased autophagy occurs. When expression is driven by Scer\GAL4Lsp2.PH, relatively late in the larval fat body, no effect on autophagic activity of fat droplet aggregation is detectable.
Flies expressing PtenScer\UAS.cGb under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa, show a consistently high heart rate; in contrast to wild-type flies, this heart rate does not decrease with age. These flies also show a low stress-induced heart failure rate that does not increase with age, again in contrast to wild-type flies. These effects are true for both male and female flies.