Flies containing eyes that are homozygous for Acn27 have rust-coloured eyes with no pseudopupil.
Acn1/Acn27 animals show a distributed lethal phase, with most larvae dying during the third instar, while some die after pupariation.
Hemocytes isolated from Acn1/Acn27 third instar larvae appear indistinguishable from wild-type hemocytes in response to a treatment which induces apoptosis in wild-type hemocytes; the mutant hemocytes undergo nuclear condensation and chromatin fragmentation.
41% of ommatidia in eyes homozygous for Acn27 have missing or extra photoreceptors or are disorganised. The mutant pigment cells contain high levels of glycogen-like particles compared to wild-type cells.
Autolysosomes are fewer and less abundant, while autophagosomes are larger and more abundant in the fat body of Acn1/Acn27 third instar larvae than in control larvae after starvation.