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General Information
Symbol
Dmel\CCT4NIG.5525R
Species
D. melanogaster
Name
FlyBase ID
FBal0274718
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
5525R
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 500bp inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing CCT4NIG.5525R under the control of Scer\GAL4ey.PU results in complete loss of eye disc field (with no effect on antennal field) in third instar larvae which, in turn, leads to the complete absence of head structures (but normal thorax and abdomen) in pharate adults.

Expressing CCT4NIG.5525R under the control of Scer\GAL4en.PU results in reduced cell proliferation and an almost complete loss of the wing disc posterior compartment, while expression under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL4nub.PU results in severely malformed wings.

Expressing CCT4NIG.5525R under the control of Scer\GAL4ptc.PU leads to increased cell death in the wing pouch, a narrower anterior-posterior compartment boundary of the wing disc and greatly reduced salivary glands in third instar larvae compared to controls. In adults, the number and size of cells in the wing blade domain between L3 and L4 veins and the overall domain size are significantly reduced compared to wild-type controls.

Expressing CCT4NIG.5525R under the control of Scer\GAL4GMR.PU fails to induce cell death in the posterior compartment of the eye disc in third instar larvae, and results in adults with normal eyes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Phenotype Manifest In
Enhanced by
Statement
Reference

CCT4NIG.5525R, Scer\GAL4ptc.PU has wing blade phenotype, enhanceable by Rheb2D1/Rheb[+]

NOT Enhanced by
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expressing CCT4UAS.cKa with CCT4NIG.5525R under the control of Scer\GAL4ptc.PU rescues the size reduction of the wing blade domain between L3 and L4 veins, while co-expression under the control of Scer\GAL4nub.PU rescues wing formation, and co-expression under the control of Scer\GAL4ey.PU fully rescues head formation defects induced by CCT4NIG.5525R expression alone.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CCT4NIG.5525R
CG5525NIG.5525R
Name Synonyms
Secondary FlyBase IDs
    References (4)