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General Information
Symbol
Dmel\Pi3K92ECAAX.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0156077
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PI3KCAAX, PI3KCA, UAS-PI3KCA, UAS-dp110CAAX
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Pi3K92E coding sequence with a farnesylation signal (CAAX) added to the 3' end were cloned into P{Express-UAS}. The encoded protein is constitutively active. Expression is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Pi3K92ECAAX.UAS under the control of Scer\GAL4ptc.PU leads to a significant increase in size of the wing blade domain between L3 and L4 veins.

Scer\GAL4ppk.PG-mediated expression of Pi3K92ECAAX.Scer\UAS in C4da neurons drives excessive dendrite branch elongation while causing little effect on patterning of C4da dendrites, resulting in dendrite arbors with a 50% increase in total dendrite length.

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal shrinkage and disappearance of pupal neuroblasts.

Expression of Pi3K92ECAAX.Scer\UAS under the control of two copies of Scer\GAL4ppk.PG produces dendrite pruning defects in ddaC neurons at 16 hours after puparium formation.

Expression of Pi3K92ECAAX.Scer\UAS driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

Ectopic expression of Pi3K92ECAAX.Scer\UAS-driven by Scer\GAL4ppk.PG causes a significant increase in dendrite coverage larval hemisegments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of Pi3K92ECAAX.Scer\UAS does not suppress the increased midgut cell size resulting from the expression of Atg9HMS01246 under the control of Scer\GAL4Myo31DF-NP0001.

Expression of Pi3K92ECAAX.Scer\UAS does not suppress the wing vein loss seen when PtpmegScer\UAS.cLa is expressed under the control of Scer\GAL4en.PU.

Dendrite growth in pathdg50/Df(3L)BSC773 animals arrests at the same value in the absence or presence of Scer\GAL4ppk.PG-mediated expression of Pi3K92ECAAX.Scer\UAS.

Co-expression of Sesnd04539 with Pi3K92ECAAX.Scer\UAS via Scer\GAL4ap-md544 suppresses the Pi3K92ECAAX.Scer\UAS hyperplastic wing phenotype.

Xenogenetic Interactions
Statement
Reference

The inflated abdomen and proboscis phenotype (that grows progressively more severe until death) induced by adult-onset expression of Hsap\APP2xAPP.SP.Scer\UAS under the control of Scer\GAL4Lk.2.2 (combined with Tub-Gal4 to limit the expression to adulthood) is completely suppressed in all flies by co-expression of Pi3K92ECAAX.Scer\UAS. Similarly, the increased apoptotic activity (detected by Casp3 immunolabelling) observed in third instar larval wing discs expressing Hsap\APP2xAPP.SP.Scer\UAS under Scer\GAL4e22c is significantly suppressed by Pi3K92ECAAX.Scer\UAS co-expression. Expresion of Hsap\APP2xAPP.SP.Scer\UAS under Scer\GAL4Toll-6-D42 either on its own or in combination with Pi3K92ECAAX.Scer\UAS does not induce cell death in larval wing discs.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pi3K92ECAAX.Scer\UAS
Pi3K92ECAAX.UAS
Name Synonyms
Secondary FlyBase IDs
    References (21)