Subject: Pi3K92E constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-Pi3K92E.Exel}, the coding region of wild type Pi3K92E
(FBgn0015279) was cloned into P{Express-UAS}.
P{UAS-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-Pi3K92E.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-Pi3K92E.Exel}, the coding region of wild type Pi3K92E was cloned
into P{Express-GMR}.
P{GMR-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{UAS-Pi3K92E.A2860C}, Pi3K92E sequences with a A2860C nucleotide change
were cloned into P{Express-UAS}. The A2860C mutation changes an Asp to Ala
in the ATP binding site and results in a dominant negative allele.
P{UAS-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{UAS-Pi3K92E.A2860C}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-Pi3K92E.A2860C}, Pi3K92E sequences with a A2860C nucleotide change
were cloned into P{Express-glass}. The A2860 mutation changes an Asp to
Ala in the ATP binding site and results in a dominant negative allele.
P{GMR-Pi3K92E.A2860C}2 is a homozygous viable and fertile, second
chromosome insertion.
P{GMR-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
In P{GMR-Pi3K92E.CAAX}, Pi3K92E coding sequence with a farnesylation signal
(CAAX) added to the 3' end were cloned into P{Express-glass}. The encoded
protein is constitutively active.
P{GMR-Pi3K92E.CAAX}2 is a second chromosome insertion.
In P{UAS-Pi3K92E.CAAX}, Pi3K92E coding sequence with a farnesylation signal
(CAAX) added to the 3' end were cloned into P{Express-UAS}. The encoded
protein is constitutively active.
P{UAS-Pi3K92E.CAAX}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.