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General Information
Symbol
Dmel\CCT4KK108551
Species
D. melanogaster
Name
FlyBase ID
FBal0235643
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing CCT4KK108551 under the control of Scer\GAL4ey.PU results in complete loss of eye disc field (with no effect on antennal field) in third instar larvae which, in turn, leads to the complete absence of head structures (but normal thorax and abdomen) in pharate adults.

Expressing CCT4KK108551 under the control of Scer\GAL4en.PU results in reduced cell proliferation and an almost complete loss of the wing disc posterior compartment, while expression under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL4nub.PU results in severely malformed wings.

Expressing CCT4KK108551 under the control of Scer\GAL4ptc.PU leads to increased cell death in the wing pouch, a narrower anterior-posterior compartment boundary of the wing disc and greatly reduced salivary glands in third instar larvae compared to controls. In adults, the number and size of cells in the wing blade domain between L3 and L4 veins and the overall domain size are significantly reduced compared to wild-type controls.

Expressing CCT4KK108551 under the control of Scer\GAL4GMR.PU fails to induce cell death in the posterior compartment of the eye disc in third instar larvae, and results in adults with normal eyes.

The expression of CCT4KK108551 under the control of either Scer\GAL4tin.CΔ4 or Scer\GAL4HCH.Hand leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to near or fully penetrant cardiac defects, respectively, namely morphological defects, non-beating heart segments and non-beating hearts, as compared to controls.

The expression of CCT4KK108551 under the control of Scer\GAL4tin.CΔ4 also leads to progressive cardiac dysfunction, as shown by significant increases in heart period, arrhythmia, and diastolic and systolic intervals, and by significant decreases in fractional shortening, and diastolic and systolic diameter, as compared to controls. These hearts show a severe and progressive disorganization in the actin- and myosin-containing myofibrils, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

CCT4KK108551, Scer\GAL4ptc.PU has wing blade phenotype, enhanceable by Rheb2D1/Rheb[+]

NOT Enhanced by
Suppressed by
NOT suppressed by
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expressing CCT4UAS.cKa with CCT4KK108551 under the control of Scer\GAL4ptc.PU rescues the size reduction of the wing blade domain between L3 and L4 veins, while co-expression under the control of Scer\GAL4nub.PU rescues wing formation, and co-expression under the control of Scer\GAL4ey.PU fully rescues head formation defects induced by CCT4KK108551 expression alone.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CCT4KK108551
CG5525KK108551
Name Synonyms
Secondary FlyBase IDs
    References (6)