FlyBase Allele Report: Dmel\wcy[A]

General Information

Symbol

Dmel\wcy^A

Species

D. melanogaster

Name

FlyBase ID

FBal0319605

Feature type

allele

Associated gene

Dmel\wcy

Associated Insertion(s)

Carried in Construct

Also Known As

XE14A

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Graves et al., 2020, David-Morrison et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: D192V.

(Graves et al., 2020, David-Morrison et al., 2016)

Amino acid replacement: R229Q.

(Graves et al., 2020, David-Morrison et al., 2016)

Amino acid replacement: K253term.

(Graves et al., 2020, David-Morrison et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:17,072,722..17,072,722 [-]

Nucleotide change:

A17072722T

Amino acid change:

Reported amino acid change:

K253term

Comment:

One of three wcy lesions identified in mutant strain. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(David-Morrison et al., 2016)

point_mutation

X:17,072,858..17,072,858 [-]

Nucleotide change:

G17072858A

Amino acid change:

Reported amino acid change:

R229Q

Comment:

One of three wcy lesions identified in mutant strain.

(David-Morrison et al., 2016)

point_mutation

X:17,073,050..17,073,050 [-]

Nucleotide change:

A17073050T

Amino acid change:

Reported amino acid change:

D192V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(David-Morrison et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

amphisome | adult stage | somatic clone

(David-Morrison et al., 2016)

autolysosome | adult stage | somatic clone

(David-Morrison et al., 2016)

autophagosome | adult stage | somatic clone

(David-Morrison et al., 2016)

autophagosome | second instar larval stage | somatic clone

(David-Morrison et al., 2016)

autophagosome | somatic clone | third instar larval stage

(David-Morrison et al., 2016)

autophagosome | somatic clone | third instar larval stage | calorie restriction conditional

(David-Morrison et al., 2016)

eye | adult stage | somatic clone

(David-Morrison et al., 2016)

eye photoreceptor cell | adult stage | progressive | somatic clone

(David-Morrison et al., 2016)

eye photoreceptor cell | adult stage | somatic clone

(David-Morrison et al., 2016)

follicle cell | somatic clone

(David-Morrison et al., 2016)

lamina | adult stage | somatic clone

(David-Morrison et al., 2016)

lysosome | adult stage | somatic clone

(David-Morrison et al., 2016)

lysosome | second instar larval stage | somatic clone

(David-Morrison et al., 2016)

lysosome | somatic clone | third instar larval stage

(David-Morrison et al., 2016)

lysosome | somatic clone | third instar larval stage | calorie restriction conditional

(David-Morrison et al., 2016)

retina | adult stage | somatic clone

(David-Morrison et al., 2016)

rhabdomere of eye photoreceptor cell | adult stage | progressive | somatic clone

(David-Morrison et al., 2016)

rhabdomere of eye photoreceptor cell | adult stage | somatic clone

(David-Morrison et al., 2016)

Detailed Description

Statement

Reference

Electroretinograms (ERGs) recorded from wcy^A/wcy^A mutant eye clones show significantly reduced amplitudes and off transients compared to controls; at day 1, mutant clones show disruption of ommatidial units, with abnormally fused or split rhabdomeres and loss of photoreceptor cells and mutant clones also show show age-dependent (1 day old versus 36 days old) degeneration of internal eye structures (defects are more severe than day 1 and numerous photoreceptors are lost, leaving vacuoles). One day old mutant retina and lamina clones show an increase in autophagosomes, amphisomes, autolysosomes and lysosomes compared to controls.

wcy^A/wcy^A mutant clones in starved early third instar larval fat bodies show decreased numbers of autophagosomes and lysosomes compared to surrounding control cells. Fed wcy^A/wcy^A mutant clones also show an increase in numbers of autophagosomes and lysosomes in fat body cells of second and third instar larvae compared to controls. Autophagosome-lysosome fusion occurs in wcy^A/wcy^A second instar larval fat bodies.

wcy^A/wcy^A full eye clones are reduced in size compared to controls. wcy^A/wcy^A mutants exhibit slow growth and developmental delay.

wcy^A/wcy^A mutants in egg chamber follicle cells show reduced clone and cell size.

(David-Morrison et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

wcy^A has abnormal neurophysiology | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has lethal - all die during second instar larval stage phenotype, suppressible by Scer\GAL4^Ubi.PU/Hsap\WAC^UAS.cDa

(David-Morrison et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

wcy^A has autophagosome | somatic clone | third instar larval stage phenotype, suppressible by Atg1^RNAi.UAS.cUa/Scer\GAL4^Cg.PU

(David-Morrison et al., 2016)

wcy^A has autophagosome | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Cg.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has retina | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has autophagosome | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has amphisome | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has autolysosome | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

wcy^A has lysosome | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/mTor^{WT.UAS.Tag:FLAG}

(David-Morrison et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg1^TRiP.cUa driven by Scer\GAL4^cg.PU suppresses increases in autophagosomes seen in wcy^A/wcy^A fat body third instar larval clones.

Expression (at 18[o]C for low level expression) of Tor^{WT.Scer\UAS.T:Zzzz\FLAG} driven in the fat body by Scer\GAL4^cg.PU suppresses the autophagy phenotype of wcy^A/wcy^A clones; expression driven in the retina by Scer\GAL4^ninaE.PU partially significantly suppresses autophagy phenotypes (increased number of autophagosomes, amphisomes, autolysosomes and lysosomes) seen in wcy^A/wcy^A clones, along with improving synaptic transmission (significantly increasing off transient amplitude).

(David-Morrison et al., 2016)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\WAC^Scer\UAS.cDa driven by Scer\GAL4^Ubi.PU rescues lethality in wcy^A/wcy^A mutants.

(David-Morrison et al., 2016)

Complementation and Rescue Data

Rescued by

wcy^A is rescued by Scer\GAL4^Ubi.PU/wcy^UAS.cDa

(David-Morrison et al., 2016)

wcy^A is rescued by Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG

(David-Morrison et al., 2016)

wcy^A is rescued by wcy^+tGR

(David-Morrison et al., 2016)

wcy^A is rescued by wcy^{Tag:FLAG,Tag:HA}

(David-Morrison et al., 2016)

wcy^A is rescued by wcy^EGFP

(David-Morrison et al., 2016)

Comments

Expression of wcy^Scer\UAS.cDa or wcy^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^Ubi.PU or wcy^+tGR, wcy^{T:Zzzz\FLAG,T:Ivir\HA1} or wcy^{T:Avic\GFP-EGFP} rescues lethality in wcy^A/wcy^A mutants. wcy^+tGR rescues retinal morphology and electroretinogram defects in wcy^A/wcy^A mutants. wcy^+tGR rescues starvation induced autophagy defects in wcy^A/wcy^A third instar larval fat body clones. wcy^+tGR, wcy^{T:Zzzz\FLAG,T:Ivir\HA1} or wcy^{T:Avic\GFP-EGFP} rescues the programmed autophagy phenotype in wcy^A/wcy^A third instar larval fat body clones.

(David-Morrison et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67177

y¹ w^* wcy^A P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

XASY045

(Graves et al., 2020)

XE14A

(Graves et al., 2020, David-Morrison et al., 2016)

wacky^A

(David-Morrison et al., 2016)

wcy^A

(Graves et al., 2020)

wcyA

(David-Morrison et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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