A genomic rescue construct containing wcy genomic sequence with additional 2400 base pairs both upstream and downstream, obtained from the bacterial artificial chromosome clone CH321-45K19, tagged with 3x EGFP at the C-terminus.
wcyEGFP rescues wcyA
wcyEGFP rescues wcyB
wcyEGFP rescues wcyC
wcyEGFP rescues wcyD
wcyEGFP rescues wcyF
wcyT:Avic\GFP-EGFP rescues lethality in wcyA/wcyA, wcyB/wcyB, wcyC/wcyC, wcyD/wcyD or wcyF/wcyF mutants. wcyT:Avic\GFP-EGFP rescues the programmed autophagy phenotype in wcyA/wcyA third instar larval fat body clones.