FlyBase Allele Report: Dmel\Pop2[GD12804]

General Information

Symbol

Dmel\Pop2^GD12804

Species

D. melanogaster

Name

FlyBase ID

FBal0208101

Feature type

allele

Associated gene

Dmel\Pop2

Associated Insertion(s)

Carried in Construct

P{GD12804}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12804}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12804

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Delta^UAS.cDa

(Vicente et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

The expression of Pop2^GD12804 under the control of Scer\GAL4^ey.PH induces a high frequency small rough eye phenotype.

(Vicente et al., 2018)

Expression of Pop2^GD12804 does not lead to wing posture or jumping ability defects (with the Scer\GAL4^Mhc.PU driver) and neither does it induce abnormal mitochondrial aggregations in dopaminergic (DA) neurons or loss of PPL1 cluster DA neurons in the adult brain (with the Scer\GAL4^ple.PF driver).

(Gehrke et al., 2015)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Pop2^GD12804 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in semi-lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12804

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pop2^GD12804, Scer\GAL4^ey.PH is an enhancer of neoplasia | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Pop2^GD12804, Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PU/Pop2^GD12804 is a suppressor | partially of visible | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/Pop2^GD12804 is a suppressor | partially of abnormal jumping phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Pop2^GD12804 is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Pop2^GD12804 is a suppressor | partially of decreased cell number | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pop2^GD12804, Scer\GAL4^ey.PH is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Pop2^GD12804 is a suppressor | partially of dopaminergic neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Pop2^GD12804 is a suppressor | partially of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Pop2^GD12804 is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/Pop2^GD12804 is a suppressor | partially of wing | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Pop2^GD12804, Scer\GAL4^Mhc.PU is a suppressor of flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Pop2^GD12804, Scer\GAL4^Mhc.PU is a suppressor of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal mitochondrial aggregations (observed in flight muscles or dopaminergic neurons), wing posture and jumping ability defects as well as the loss of dopaminergic neurons from the PPL1 cluster characteristic for Pink1^B9 adult homozygotes are significantly improved by expression (controlled by either the Scer\GAL4^Mhc.PU muscle driver or the Scer\GAL4^ple.PF neuronal driver) of Pop2^GD12804. Similarly, the abnormal mitochondrial morphology in the flight muscles of adult flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PU is partially suppressed by co-expression of Pop2^GD12804.