FlyBase Allele Report: Dmel\eIF4G1[HMS00762]

General Information

Symbol

Dmel\eIF4G1^HMS00762

Species

D. melanogaster

Name

FlyBase ID

FBal0257445

Feature type

allele

Associated gene

Dmel\eIF4G1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00762}

Also Known As

eIF4G RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00762}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00762

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates retinitis pigmentosa 4

modeled by ninaE^P37H.GFP

(Zhao et al., 2023)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Ghosh and Lasko, 2015)

lethal, with Scer\GAL4^Ubi.PU

(Ghosh and Lasko, 2015)

male fertile, with Scer\GAL4^VP16.bam

(Ghosh and Lasko, 2015)

male fertile, with Scer\GAL4^{VP16.nanos.UTR}

(Ghosh and Lasko, 2015)

male semi-sterile, with Scer\GAL4^C587

(Ghosh and Lasko, 2015)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

elongation stage spermatid, with Scer\GAL4^C587

(Ghosh and Lasko, 2015)

primary spermatocyte cyst, with Scer\GAL4^C587

(Ghosh and Lasko, 2015)

testis, with Scer\GAL4^C587

(Ghosh and Lasko, 2015)

Detailed Description

Statement

Reference

Expression of glo^{dsRNA.Scer\UAS.cUa} does not lead to wing posture or jumping ability defects (with the Scer\GAL4^Mhc.PU driver) and but it does loss of dopaminergic neurons from the PPL1 cluster in the adult brain (with the Scer\GAL4^ple.PF driver).

(Gehrke et al., 2015)

Scer\GAL4^{nos.UTR.T:Hsim\VP16}- or Scer\GAL4^{bam.T:Hsim\VP16}-mediated expression of eIF4G^HMS00762 does not result in sterility or abnormal testes in males. However, Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of eIF4G^HMS00762in females results arrests oogenesis at pre-vitellogenic stages.

Scer\GAL4^C587 eIF4G^HMS00762 males produce significantly fewer progeny. In a majority of the mutant testes, primary spermatocyte cysts show decreased spatial organization and over-proliferation. As compared to wild-type, meiotic and post-meiotic stages are under-represented and sperm maturation also appears to be affected. Nuclei with varying degrees of condensation are found dispersed in the elongated spermatids, and needle-like sperm nuclear bundles are detected both at the caudal and rostral end of the sperm tails.

(Ghosh and Lasko, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

eIF4G1^HMS00762/Scer\GAL4^Mhc.PU is an enhancer of visible | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

eIF4G1^HMS00762/Scer\GAL4^Mhc.PU is an enhancer of abnormal jumping phenotype of Pink1^B9

(Gehrke et al., 2015)

Other

Statement

Reference

Pink1^B9, Scer\GAL4^ple.PF, eIF4G1^HMS00762 has lethal phenotype

(Gehrke et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}, eIF4G1^HMS00762, eIF4G2^GL00469 has male sterile phenotype

(Ghosh and Lasko, 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

eIF4G1^HMS00762/Scer\GAL4^Mhc.PU is an enhancer of wing | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

eIF4G1^HMS00762, Scer\GAL4^Mhc.PU is an enhancer of flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

eIF4G1^HMS00762, Scer\GAL4^Mhc.PU is an enhancer of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, eIF4G1^HMS00762, eIF4G2^GL00469 has testis phenotype

(Ghosh and Lasko, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posture and jumping ability defects characteristic for Pink1^B9 adult homozygotes are exacerbated by expression (controlled by either the Scer\GAL4^Mhc.PU muscle driver) of eIF4G1^HMS00762; Scer\GAL4^ple.PF-driven expression of eIF4G1^HMS00762 in the Pink1^B9 mutant background is lethal. Similarly, the abnormal mitochondrial morphology in the flight muscles of adult flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PU is worsened further by co-expression of eIF4G1^HMS00762.

(Gehrke et al., 2015)

Males expressing both eIF4G^HMS00762 and eIF4G2^GL00469 via Scer\GAL4^{nos.UTR.T:Hsim\VP16} are sterile and produce rudimentary testes. The apical tips are filled with masses of dividing cells, indicating an arrest before the primary spermatocyte stage.

(Ghosh and Lasko, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33049

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00762}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eIF4G1^HMS00762

eIF4G^HMS00762

eIF4G^RNAi

(Zhao et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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