FlyBase Allele Report: Dmel\park[SA.UAS]

General Information

Symbol

Dmel\park^SA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0302843

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

P{UAS-park.SA}

Also Known As

UAS-dParkin S94A

Key Links

Transgenic product class

missense_variant

(Shiba-Fukushima et al., 2014)

polypeptide_post_translational_processing_variant

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Shiba-Fukushima et al., 2014)

polypeptide_post_translational_processing_variant

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

(Shiba-Fukushima et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-park.SA}

Cytology

Description

UASt regulatory sequences drive expression of a non-phospho form of park in which the serine at amino acid 94 has been replaced by alanine.

(Shiba-Fukushima et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

park

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

short lived, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of park^SA.Scer\UAS in indirect flight muscles under the control of Scer\GAL4^Mhc.PU does not have a significant effect on mitochondria length compared to controls, but mitochondrial morphology is less uniform. ATP levels are similar to controls.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^Mhc.PU improves the decline in climbing ability usually seen in older flies.

The indirect flight muscles of flies expressing park^SA.Scer\UAS under the control of Scer\GAL4^da.PU in a park^Δ21/park¹ mutant background contain abnormally fused mitochondria.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^ple.PU has no effect on the distribution and morphology of the mitochondria in dopaminergic neurons in 5 day old flies compared to controls.

Flies expressing park^SA.Scer\UAS under the control of Scer\GAL4^ple.PU exhibit reduced flying ability compared to controls.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^ple.PU results in an age-dependent loss of dopaminergic neurons.

(Shiba-Fukushima et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ple.PU, park^SA.UAS has abnormal neuroanatomy phenotype, enhanceable by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

park^SA.UAS/Scer\GAL4^Mhc.PU is a suppressor of short lived phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^SA.UAS/Scer\GAL4^Mhc.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ple.PU, park^SA.UAS has dopaminergic neuron phenotype, enhanceable by Pink1^B9

(Shiba-Fukushima et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^ple.PU, park^SA.UAS has dopaminergic neuron phenotype, non-suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU, park^SA.UAS has mitochondrion phenotype, non-suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

park^SA.UAS/Scer\GAL4^Mhc.PU is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^SA.UAS/Scer\GAL4^Mhc.PU is a suppressor of mitochondrion phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU/park^SA.UAS is a suppressor of mitochondrion phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU/park^SA.UAS is a suppressor of dopaminergic neuron phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormally large, fused mitochondria observed in Pink1^B9 mutant flies are completely rescued when park^SA.Scer\UAS is expressed under the control of Scer\GAL4^Mhc.PU.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^Mhc.PU suppresses the reduction in ATP levels seen in Pink1^B9 mutant flies. The reduction in climbing ability and wing and thorax defects are also rescued.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^ple.PU rescues the mitochondrial aggregation and distribution defects seen in Pink1^B9 mutant dopaminergic neurons.

Flies expressing park^SA.Scer\UAS under the control of Scer\GAL4^ple.PU exhibit reduced flying ability compared to controls.

Pink1^B9 enhances the dopaminergic neuron loss seen when park^SA.Scer\UAS is expressed under the control of Scer\GAL4^ple.PU.

(Shiba-Fukushima et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

park^SA.UAS/Scer\GAL4^Mhc.PU partially rescues park^Δ21/park¹

(Shiba-Fukushima et al., 2014)

Fails to rescue

Scer\GAL4^da.PU/park^SA.UAS fails to rescue park^Δ21/park¹

(Shiba-Fukushima et al., 2014)

Comments

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^da.PU fails to rescue the mitochondria phenotype seen in park^Δ21/park¹ mutant indirect flight muscles. The reduction in ATP levels is rescued, and is improved to a level higher than that of controls.

Expression of park^SA.Scer\UAS under the control of Scer\GAL4^Mhc.PU partially rescues the wing posture and thorax defects seen in park^Δ21/park¹ mutant flies. The reduction in ATP levels is also improved.

(Shiba-Fukushima et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

park^SA.Scer\UAS

park^SA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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