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General Information
Symbol
Dmel\NUAS.cUa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Unknown
FlyBase ID
FBal0215932
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-N, UAS Notch, UAS-Notch
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of full-length N.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of NScer\UAS.cUa under the control of Scer\GAL4ple.PU as well as the Scer\GAL4elav.PU driver in combination with Scer\GAL80ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan and, with the Scer\GAL4ple.PU driver, loss of dopaminergic neurons from the PPL1, PPM1/2 and PPM3 clusters in the adult brain.

Nl1N-ts1 animals raised at the restrictive temperature from 12 hours after puparium formation onwards and expressing NScer\UAS.cUa under the control of Scer\GAL4neur-GAL4-A101 (to restore N activity selectively in the sensory organs) show normal development of the majority of mechanosensory organs of the leg, and each of these correctly induces a single proximal bract.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Scer\GAL4hh-Gal4-mediated expression of NScer\UAS.cUa enhances the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1d7 wing discs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of NScer\UAS.cUa under the control of Scer\GAL4repo-M1B efficiently rescues the early axon phenotype of Nl1N-ts1 and restores restores the continuity of the neuronal meshwork.

Expression of NScer\UAS.cUa under the control of Scer\GAL4htl.PM efficiently rescues the early axon phenotype of Nl1N-ts1.

Expression of NScer\UAS.cUa under the control of Scer\GAL415J2 efficiently rescues the early axon phenotype of Nl1N-ts1.

Scer\GAL4da.G32-mediated expression of NScer\UAS.cUa significantly rescues the neurogenic phenotype of Df(1)N-54l9 embryos, and suppresses the notched wing phenotype of Df(1)N-54l9/+ flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NScer\UAS.cUa
NUAS.cUa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs
    References (11)