FlyBase Allele Report: Dmel\erm[1]

General Information

Symbol

Dmel\erm¹

Species

D. melanogaster

Name

FlyBase ID

FBal0242760

Feature type

allele

Associated gene

Dmel\erm

Associated Insertion(s)

Carried in Construct

Also Known As

dFezf¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Weng et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: H392L.

(Lee, 2012.12.18)

erm¹ is a single A to T nucleotide change in the erm coding region, leading to the substitution of a leucine for histidine.

(Weng et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,951,717..1,951,717 [-]

Nucleotide change:

A1951717T

Amino acid change:

H392L | erm-PA; H384L | erm-PB; H392L | erm-PC; H392L | erm-PD

Reported amino acid change:

H392L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee, 2012.12.18, Weng et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

lamina monopolar neuron L3 | adult stage | somatic clone

(Santiago et al., 2021)

lamina monopolar neuron L3 | P-stage | somatic clone

(Santiago et al., 2021)

larval brain | larval stage (with Df(2L)erm²)

(Rives-Quinto et al., 2020)

larval neuroblast (with Df(2L)erm²)

(Weng et al., 2010)

larval neuroblast | ectopic | increased number

(Weng et al., 2010)

larval neuroblast | ectopic | somatic clone | increased number

(Weng et al., 2010)

larval neuroblast | larval stage (with Df(2L)erm²)

(Weng et al., 2010)

outer medulla | adult stage | somatic clone

(Santiago et al., 2021)

outer medulla | P-stage | somatic clone

(Santiago et al., 2021)

type II neuroblast | increased number (with Df(2L)erm²), with erm^Tag:FLAG

(Janssens et al., 2014)

type II neuroblast | increased number | larval stage (with Df(2L)erm²)

(Rives-Quinto et al., 2020)

type II neuroblast | somatic clone | increased number (with Df(2L)erm²)

(Janssens et al., 2014)

Detailed Description

Statement

Reference

No excess neuroblasts are seen in erm¹ mutant brains.

erm¹/Df(2L)erm² mutant clones originating from either a single ase+ immature Intermediate Neural Progenitor (INP) or INP contain an increased number of neuroblasts 'reverted clones' compared to controls.

Supernumerary neuroblasts are seen in hypomorphic erm mutant larval brains (i.e. erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

erm¹/Df(2L)erm² mutant clones originating from a single type II neuroblast contain multiple neuroblasts per clone.

(Janssens et al., 2014)

erm¹ larval brains contain over 20-fold more type II neuroblasts than wild type brains, with no significant change in the number of type I neuroblasts.

48 hours after induction, erm¹ type II neuroblast clones in larval brains contain fewer Intermediate Neural Progenitors and multiple, ectopic type II neuroblasts compared to wild type clones.

72 hours after induction, marked Intermediate Neural Progenitor clones in the larval brain in a wild type background contain only differentiated neurons. In contrast, similar marked clones in a erm¹/Df(2L)erm² background contain one or more de-differentiated type II neuroblasts as well as immature Intermediate Neural Progenitors, Intermediate Neural Progenitors, Ganglion Mother Cells and neurons.

erm¹ mutant larval brain hemispheres contain more than 50 glial chambers, each containing one or more type II neuroblasts and their progeny. This is in contrast to wild type hemispheres that contain 8 glial chambers encapsulating eight individual type II neuroblast lineages.

(Weng et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by dpn^UAS.cWa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^wor.PA/brm^{K804R.UAS.Tag:HA}

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by klu^UAS.Tag:HA/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Suppressed by

Statement

Reference

Df(2L)erm²/erm¹ has increased cell number | larval stage phenotype, suppressible by Scer\GAL4^erm.PU/ham^UAS.cMa

(Rives-Quinto et al., 2020)

Df(2L)erm²/erm¹ has increased cell number | larval stage phenotype, suppressible by ham^{ΔC-ZF.UAS.Tag:MYC}/Scer\GAL4^erm.PU

(Rives-Quinto et al., 2020)

brat^DG19310/brat¹¹, erm[+]/erm¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Janssens et al., 2014)

Df(2L)erm²/erm¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by klu^212lR51C

(Janssens et al., 2014)

Df(2L)erm²/erm¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by dpn¹

(Janssens et al., 2014)

erm¹ has abnormal neuroanatomy phenotype, suppressible by pros^L.UAS/Scer\GAL4^GMR9D11

(Weng et al., 2010)

erm¹ has abnormal neuroanatomy phenotype, suppressible | partially by N^GD14477/Dcr-2^UAS.cDa/Scer\GAL4^wor.PA

(Weng et al., 2010)

Enhancer of

Statement

Reference

erm[+]/erm¹ is an enhancer of increased cell number | larval stage phenotype of HDAC3^HMS00087, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Rives-Quinto et al., 2020)

erm[+]/erm¹ is an enhancer of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

erm[+]/erm¹ is an enhancer of abnormal neuroanatomy phenotype of numb^NP2301

(Janssens et al., 2014)

Other

Statement

Reference

Df(2L)slp^S37A, erm¹ has abnormal neuroanatomy | somatic clone | adult stage phenotype

(Santiago et al., 2021)

Df(2L)slp^S37A, erm¹ has abnormal neuroanatomy | somatic clone | P-stage phenotype

(Santiago et al., 2021)

brat^DG19310/brat¹¹, erm[+]/erm¹ has abnormal neuroanatomy | somatic clone phenotype

(Janssens et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by Scer\GAL4^wor.PA/brm^{K804R.UAS.Tag:HA}

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by klu^UAS.Tag:HA/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by dpn^UAS.cWa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Suppressed by

Statement

Reference

Df(2L)erm²/erm¹ has type II neuroblast | increased number | larval stage phenotype, suppressible by Scer\GAL4^erm.PU/ham^UAS.cMa

(Rives-Quinto et al., 2020)

Df(2L)erm²/erm¹ has type II neuroblast | increased number | larval stage phenotype, suppressible by ham^{ΔC-ZF.UAS.Tag:MYC}/Scer\GAL4^erm.PU

(Rives-Quinto et al., 2020)

Df(2L)erm²/erm¹ has larval brain | larval stage phenotype, suppressible by Scer\GAL4^erm.PU/ham^UAS.cMa

(Rives-Quinto et al., 2020)

Df(2L)erm²/erm¹ has larval brain | larval stage phenotype, suppressible by ham^{ΔC-ZF.UAS.Tag:MYC}/Scer\GAL4^erm.PU

(Rives-Quinto et al., 2020)

brat^DG19310/brat¹¹, erm[+]/erm¹ has type II neuroblast | somatic clone phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Janssens et al., 2014)

Df(2L)erm²/erm¹ has type II neuroblast | increased number | somatic clone phenotype, suppressible by klu^212lR51C

(Janssens et al., 2014)

Df(2L)erm²/erm¹ has type II neuroblast | increased number | somatic clone phenotype, suppressible by dpn¹

(Janssens et al., 2014)

erm¹ has larval neuroblast | ectopic | increased number phenotype, suppressible by pros^L.UAS/Scer\GAL4^GMR9D11

(Weng et al., 2010)

erm¹ has larval neuroblast | ectopic | increased number phenotype, suppressible | partially by N^GD14477/Dcr-2^UAS.cDa/Scer\GAL4^wor.PA

(Weng et al., 2010)

Enhancer of

Statement

Reference

erm[+]/erm¹ is an enhancer of type II neuroblast | larval stage | increased number phenotype of HDAC3^HMS00087, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Rives-Quinto et al., 2020)

erm[+]/erm¹ is an enhancer of larval brain | larval stage phenotype of HDAC3^HMS00087, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Rives-Quinto et al., 2020)

erm[+]/erm¹ is an enhancer of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

erm[+]/erm¹ is an enhancer of type II neuroblast | increased number phenotype of numb^NP2301

(Janssens et al., 2014)

Other

Statement

Reference

Df(2L)slp^S37A, erm¹ has lamina monopolar neuron L3 | somatic clone | adult stage phenotype

(Santiago et al., 2021)

Df(2L)slp^S37A, erm¹ has lamina monopolar neuron L3 | somatic clone | P-stage phenotype

(Santiago et al., 2021)

Df(2L)slp^S37A, erm¹ has outer medulla | somatic clone | adult stage phenotype

(Santiago et al., 2021)

Df(2L)slp^S37A, erm¹ has outer medulla | somatic clone | P-stage phenotype

(Santiago et al., 2021)

brat^DG19310/brat¹¹, erm[+]/erm¹ has type II neuroblast | increased number | somatic clone phenotype

(Janssens et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of erm¹ enhances the supernumerary type II neuroblast phenotype seen in brat^DG19310/brat¹¹ mutants.

In contrast to brat^DG19310/brat¹¹ clones alone, clones originating from either a single ase+ immature Intermediate Neural Progenitor (INP) or INP that are also heterozygous for erm¹ contain an increased number of neuroblasts 'reverted clones'. This phenotype is reversed upon expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR9D11. This rescue is seen using an insertion line of the driver that expressed in ase[-] INPs (P{GMR9D11-GAL4}2) and one that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

Expression of klu^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Homozygous klu^212lR51C suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant clones originating from a single type II neuroblast.

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Homozygous dpn¹ suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant clones originating from a single type II neuroblast.

Expression of E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

One copy of erm¹ enhances the supernumerary neuroblast phenotype seen in numb^NP2301 mutant larval brains.

Expression of brm^{K804R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wor.PA enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

(Janssens et al., 2014)

Expression of pros^L.Scer\UAS under the control of Scer\GAL4^{CG31670.R9D11} suppresses ectopic neuroblasts and restores neuronal differentiation in erm¹ larval brains.

Expression of N^GD14477 (together with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^wor.PA in erm¹ larval brains leads to a dramatic reduction in ectopic type II neuroblasts compared to erm¹ brains alone.

(Weng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

erm¹ is rescued by Scer\GAL4^9-9/erm^UAS.Tag:HA

(Santiago et al., 2021)

erm¹ is rescued by Scer\GAL4^9-9/erm^zf.ERD.UAS

(Santiago et al., 2021)

Df(2L)erm²/erm¹ is rescued by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is rescued by Scer\GAL4^GMR9D11/erm^zf.ERD.UAS

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is rescued by erm^Tag:FLAG/Scer\GAL4^GMR9D11/erm^zf.ERD.UAS

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is rescued by Scer\GAL4^GMR9D11/erm^zf1.2A.UAS

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is rescued by erm^zf5.2A.UAS/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Not rescued by

erm¹ is not rescued by erm^zf.VP16.UAS/Scer\GAL4^9-9

(Santiago et al., 2021)

Df(2L)erm²/erm¹ is not rescued by Scer\GAL4^GMR9D11/erm^zf2.2A.UAS

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is not rescued by Scer\GAL4^GMR9D11/erm^zf3.2A.UAS

(Janssens et al., 2014)

Df(2L)erm²/erm¹ is not rescued by Scer\GAL4^GMR9D11/erm^zf4.2A.UAS

(Janssens et al., 2014)

Comments

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf.Scer\UAS.ERD} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf.Scer\UAS.ERD} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) rescues the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of erm^{zf1.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) rescues the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf2.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) does not rescue the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf3.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) does not rescue the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf4.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) does not rescue the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf5.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) rescues the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf6.2A.Scer\UAS} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) rescues the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

(Janssens et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

dFezf¹

(Santiago et al., 2021, Peng et al., 2018)

erm¹

(Konstantinides et al., 2022, Rives-Quinto et al., 2020, Janssens et al., 2017, Li et al., 2017, Janssens et al., 2014, Komori et al., 2014, Lee, 2012.12.18, Weng et al., 2010)

erm^l(2)5138

(Larson et al., 2021)

l(2)5138

(Weng et al., 2010)

l(2)5138¹

(Weng et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (11)

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