FlyBase Allele Report: Dmel\erm[Tag:FLAG]

General Information

Symbol

Dmel\erm^Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0297354

Feature type

allele

Associated gene

Dmel\erm

Associated Insertion(s)

Carried in Construct

P{erm-FLAG}

Also Known As

erm-flag

Key Links

Transgenic product class

genomic_DNA

(Janssens et al., 2014)

wild_type

(Janssens et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Janssens et al., 2014)

wild_type

(Janssens et al., 2014)

Mutagen

in vitro construct

(Janssens et al., 2014)

Progenitor genotype

Carried in construct

P{erm-FLAG}

Cytology

Description

A genomic fragment containing the erm locus and fused in frame to Tag:FLAG.

(Janssens et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

erm

Encoded product / tool

erm

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Df(2L)erm²/erm¹

(Janssens et al., 2014)

Phenotype Manifest In

type II neuroblast | increased number, with Df(2L)erm²/erm¹

(Janssens et al., 2014)

Detailed Description

Statement

Reference

Supernumerary neuroblasts are seen in hypomorphic erm mutant larval brains (i.e. erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

(Janssens et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by klu^UAS.Tag:HA/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by dpn^UAS.cWa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^wor.PA/brm^{K804R.UAS.Tag:HA}

(Janssens et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by klu^UAS.Tag:HA/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by dpn^UAS.cWa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Df(2L)erm²/erm¹, erm^Tag:FLAG has type II neuroblast | increased number phenotype, enhanceable by Scer\GAL4^wor.PA/brm^{K804R.UAS.Tag:HA}

(Janssens et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of klu^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of brm^{K804R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wor.PA enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

(Janssens et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

erm^Tag:FLAG, Scer\GAL4^GMR9D11, erm^zf.ERD.UAS rescues Df(2L)erm²/erm¹

(Janssens et al., 2014)

Comments

Expression of erm^{zf.Scer\UAS.ERD} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) rescues the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

(Janssens et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

erm^T:Zzzz\FLAG

erm^Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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