FlyBase Allele Report: Dmel\erm[zf.VP16.UAS]

General Information

Symbol

Dmel\erm^zf.VP16.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0297353

Feature type

allele

Associated gene

Dmel\erm

Associated Insertion(s)

Carried in Construct

P{UAS-erm.zf.ERD}

P{UAS-erm.zf.VP16}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Janssens et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-erm.zf.ERD}

P{UAS-erm.zf.VP16}

Cytology

Description

UAS regulatory sequences drive expression of a mutant form of erm containing only the zinc finger domains, fused at the N-terminal end to the VP16 activation domain, converting it to act solely as a transcriptional activator.

(Janssens et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

erm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^wor.PA

(Janssens et al., 2014)

Phenotype Manifest In

type II neuroblast | increased number | third instar larval stage, with Scer\GAL4^wor.PA

(Janssens et al., 2014)

Detailed Description

Statement

Reference

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^wor.PA results in a mild increase in the number of type II neuroblasts in the larval brain lobe.

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) does not produce any supernumerary type II neuroblasts in the larval brain.

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

(Janssens et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

erm^zf.VP16.UAS/Scer\GAL4^GMR9D11 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR9D11, dpn^UAS.cWa

(Janssens et al., 2014)

Other

Statement

Reference

Scer\GAL4^GMR9D11, erm^zf.VP16.UAS, klu^UAS.Tag:HA has abnormal neuroanatomy phenotype

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11, erm^zf.VP16.UAS has abnormal neuroanatomy phenotype

(Janssens et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

erm^zf.VP16.UAS/Scer\GAL4^GMR9D11 is an enhancer of type II neuroblast phenotype of Scer\GAL4^GMR9D11, dpn^UAS.cWa

(Janssens et al., 2014)

Other

Statement

Reference

Scer\GAL4^GMR9D11, erm^zf.VP16.UAS, klu^UAS.Tag:HA has type II neuroblast | increased number phenotype

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11, erm^zf.VP16.UAS has type II neuroblast | increased number phenotype

(Janssens et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to when either transgene is expressed alone, co-expression of erm^{zf.Scer\UAS.T:Hsim\VP16} and klu^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) results in supernumerary type II neuroblasts in the larval brain.

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} enhances the supernumerary neuroblast phenotype seen in larval brains when dpn^Scer\UAS.cWa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2).

In contrast to when either transgene is expressed alone, co-expression of erm^{zf.Scer\UAS.T:Hsim\VP16} and E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) results in supernumerary type II neuroblasts in the larval brain.

(Janssens et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

erm^zf.VP16.UAS/Scer\GAL4^9-9 fails to rescue erm¹

(Santiago et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

erm^{zf.Scer\UAS.T:Hsim\VP16}

erm^zf.VP16.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close