FlyBase Allele Report: Dmel\dpn[1]

General Information

Symbol

Dmel\dpn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0031051

Feature type

allele

Associated gene

Dmel\dpn

Associated Insertion(s)

P{lacW}dpn¹

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bier et al., 1992)

Mutagen

P-element activity

(Bier et al., 1992)

Progenitor genotype

Associated Insertion(s)

P{lacW}dpn¹

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic lobe | third instar larval stage

(Wallace et al., 2000)

lamina | third instar larval stage

(Wallace et al., 2000)

neuroblast MBp | larval stage (with dpn⁷)

(Zhu et al., 2012)

type II neuroblast | larval stage (with dpn⁷)

(Zhu et al., 2012)

type I neuroblast | third instar larval stage (with dpn⁷)

(Zhu et al., 2012)

Detailed Description

Statement

Reference

No excess neuroblasts are seen in dpn¹ mutant brains.

(Janssens et al., 2014)

Type II neuroblasts are completely absent in the brains of late third larval instar dpn¹/dpn⁷ larvae. The number of type I neuroblasts is close to that of wild type at 9-12 hours after larval hatching (ALH), but is reduced compared to wild type at 48 hours ALH, and does not reduce further from 48 hours to 96 hours ALH.

Mushroom body neuroblasts are progressively lost in dpn¹/dpn⁷ mutants: at the late third instar larval stage nearly half of the mushroom body neuroblasts are missing, and by the start of pupariation, 90% of them are lost.

(Zhu et al., 2012)

In the optic nerve of dpn¹ homozygous third instar larvae,sporadic breaks are evident in the normally continuous area of S phase positive cells. These breaks can vary in size and location in the outer proliferation center (OPC) of the developing optic lobe, but can be found in the OPC of nearly all homozygous dpn¹ mutant larvae. In addition, S-phase activity in the developing lamina appears compressed and disorganized. The number of photoreceptor axons in dpn¹ and wild type mutant remains similar, however the number of lamina cells is decreased.

(Wallace et al., 2000)

Viability of homozygous females: 13%. Viability of homozygous males: 20%.

(Barbash and Cline, 1995)

Homozygotes die at various stages of development, with occasional adult survivors when conditions are especially favourable. Homozygous larvae are weak, but there are no consistant morphological defects when examined with antibodies that recognise neuronal nuclei, membranes or neuroblasts, or PNS cytoplasm, neuronal precursors or muscles. More than 50% of the males doubly heterozygous for dpn¹ and a duplication for the achaete-scute complex die.

(Bier et al., 1992)

Flies homozygous for dpn¹ or heterozygous for dpn¹ and a deficiency show reduced viability and die at various stages of development: viabilities relative to controls are 1.5% in males and 8-10% in females. Males with 2 or 3 copies of sc⁺ and homozygous for dpn¹ were never recovered. Females with 3 copies of sc⁺ and homozygous for dpn¹ have improved viability whereas females with three copies of dpn⁺ and one copy of sc⁺ show reduced viability. dpn¹ larvae and adults show limited movement and are extremely lethargic.

(Younger-Shepherd et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dpn⁷/dpn¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by pros[+]/pros^V17

(Zhu et al., 2012)

Enhancer of

Statement

Reference

dpn[+]/dpn¹ is an enhancer of visible | dominant phenotype of N^55e11

(Babaoğlan et al., 2013)

Suppressor of

Statement

Reference

dpn[+]/dpn¹ is a suppressor of increased cell number | larval stage phenotype of brat^DG19310/brat¹¹

(Komori et al., 2025, Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of increased cell number | larval stage phenotype of Cul1^HM05197, Scer\GAL4^wor.PA, Scer\GAL80^ase.PU

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of increased cell number | larval stage phenotype of brat^DG19310/brat¹¹, insb^exA45

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of increased cell number | larval stage phenotype of Tis11^IE35, brat^DG19310/brat¹¹

(Komori et al., 2018)

dpn¹ is a suppressor of abnormal neuroanatomy | somatic clone phenotype of Df(2L)erm²/erm¹

(Janssens et al., 2014)

Other

Statement

Reference

dpn¹, scrt^jo11 has lethal | recessive phenotype

(Roark et al., 1995)

Phenotype Manifest In

Suppressed by

Statement

Reference

dpn⁷/dpn¹ has type I neuroblast | third instar larval stage phenotype, suppressible | partially by pros[+]/pros^V17

(Zhu et al., 2012)

Enhancer of

Statement

Reference

dpn[+]/dpn¹ is an enhancer of wing margin phenotype of N^55e11

(Babaoğlan et al., 2013)

dpn[+]/dpn¹ is an enhancer of eye phenotype of scrt^jo11

(Roark et al., 1995)

dpn[+]/dpn¹ is an enhancer of eye photoreceptor cell phenotype of scrt^jo11

(Roark et al., 1995)

Suppressor of

Statement

Reference

dpn[+]/dpn¹ is a suppressor of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Komori et al., 2025)

dpn[+]/dpn¹ is a suppressor of type II neuroblast | increased number | larval stage phenotype of brat^DG19310/brat¹¹, insb^exA45

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of embryonic/larval optic lobe | larval stage phenotype of brat^DG19310/brat¹¹, insb^exA45

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of type II neuroblast | increased number | larval stage phenotype of brat^DG19310/brat¹¹

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of embryonic/larval optic lobe | larval stage phenotype of brat^DG19310/brat¹¹

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of type II neuroblast | increased number | larval stage phenotype of Tis11^IE35, brat^DG19310/brat¹¹

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of embryonic/larval optic lobe | larval stage phenotype of Tis11^IE35, brat^DG19310/brat¹¹

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of type II neuroblast | increased number | larval stage phenotype of Cul1^HM05197, Scer\GAL4^wor.PA, Scer\GAL80^ase.PU

(Komori et al., 2018)

dpn[+]/dpn¹ is a suppressor of embryonic/larval optic lobe | larval stage phenotype of Cul1^HM05197, Scer\GAL4^wor.PA, Scer\GAL80^ase.PU

(Komori et al., 2018)

dpn¹ is a suppressor of type II neuroblast | increased number | somatic clone phenotype of Df(2L)erm²/erm¹

(Janssens et al., 2014)

Other

Statement

Reference

dpn¹, scrt^jo11 has presumptive embryonic/larval peripheral nervous system phenotype

(Roark et al., 1995)

dpn¹, scrt^jo11 has presumptive embryonic/larval central nervous system phenotype

(Roark et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous dpn¹ suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant clones originating from a single type II neuroblast.

(Janssens et al., 2014)

dpn¹/+ enhances the wing notching phenotype seen in N^55e11/+ flies (78% of flies have wing nicks).

dpn¹/+ enhances the mild wing vein phenotype of Df(3R)E(spl)Δmγ-mβ-DK33-10.1 flies, such that the double mutant has prominent vein thickening at the tips of veins L4 and L5 as well as ectopic veins flanking vein L5 in 26% of wings.

(Babaoğlan et al., 2013)

pros^V17/+ partially rescues the reduction in number of type I neuroblasts which is seen in the brains of late third larval instar dpn¹/dpn⁷ larvae.

(Zhu et al., 2012)

Whereas either single mutant shows escapers, dpn¹, scrt^jo11 double mutant embryos never hatch and exhibit a dramatic reduction of both central and peripheral nervous system nervous system. While eyes of dpn¹ are normal, dpn¹/+,scrt^jo11 have an enhanced rough eye phenotype and more severe loss of photoreceptors than for scrt^jo11 alone.

(Roark et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Viability of dpn¹/dpn⁶ is unexpectedly high, perhaps due to transvection between the alleles.

(Barbash and Cline, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dpn1

(Mavromatakis and Tomlinson, 2016)

dpn¹

(Komori et al., 2025, Larson et al., 2021, Komori et al., 2018, Janssens et al., 2017, Li et al., 2017, Janssens et al., 2014, Babaoğlan et al., 2013, Zhu et al., 2012, Wallace et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (14)

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