FlyBase Allele Report: Dmel\dpn[7]

General Information

Symbol

Dmel\dpn⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0049433

Feature type

allele

Associated gene

Dmel\dpn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

gamma ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Barbash and Cline, 1995)

Mutagen

gamma ray

(Barbash and Cline, 1995)

Progenitor genotype

Cytology

Description

Amino acid replacement: K45N. K45N falls in the basic region of the bHLH region. Nucleotide substitution: A404C (nucleotide positions as in cDNA sequence of FBrf0056120).

(Barbash and Cline, 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:8,231,686..8,231,686 [-]

Nucleotide change:

A8231686C

Reported nucleotide change:

A404C

Amino acid change:

K45N | dpn-PA

Reported amino acid change:

K45N

(Barbash and Cline, 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Zhu et al., 2012)

abnormal neuroanatomy | larval stage (with dpn¹)

(Zhu et al., 2012)

abnormal neuroanatomy | somatic clone

(Zhu et al., 2012)

flightless (with Df(2R)dpn-2)

(San-Juán and Baonza, 2011)

lethal | recessive

(Barbash and Cline, 1995)

partially lethal - majority live (with Df(2R)dpn-2)

(San-Juán and Baonza, 2011)

uncoordinated (with Df(2R)dpn-2)

(San-Juán and Baonza, 2011)

Phenotype Manifest In

neuroblast MBp | larval stage (with dpn¹)

(Zhu et al., 2012)

neuroblast MBp | somatic clone

(Zhu et al., 2012)

type II neuroblast | larval stage

(Zhu et al., 2012)

type II neuroblast | larval stage (with Df(2R)dpn-2)

(San-Juán and Baonza, 2011)

type II neuroblast | larval stage (with dpn¹)

(Zhu et al., 2012)

type II neuroblast | larval stage | somatic clone

(San-Juán and Baonza, 2011)

type I neuroblast | third instar larval stage (with dpn¹)

(Zhu et al., 2012)

Detailed Description

Statement

Reference

Type II neuroblasts are completely absent in the brains of late third larval instar dpn¹/dpn⁷ larvae. The number of type I neuroblasts is close to that of wild type at 9-12 hours after larval hatching (ALH), but is reduced compared to wild type at 48 hours ALH, and does not reduce further from 48 hours to 96 hours ALH.

Mushroom body neuroblasts are progressively lost in dpn¹/dpn⁷ mutants: at the late third instar larval stage nearly half of the mushroom body neuroblasts are missing, and by the start of pupariation, 90% of them are lost.

Homozygous mushroom body neuroblast clones generated immediately after larval hatching have mostly lost their neuroblasts by 1-2 days after pupariation, in contrast to wild-type control clones, which always contain a neuroblast at this stage.

Heterozygous larvae show a slight decrease in the number of type II neuroblasts in the brain compared to wild type.

(Zhu et al., 2012)

The few Df(2R)dpn-2/dpn⁷ mutant escapers that are seen are uncoordinated and flightless. The total number of neuroblasts is reduced during the larval stages. Type II neuroblasts are never observed at late stages of larval development although one is occasionally seen in early larval brains.

dpn⁷ mutant clones generated in type II neuroblasts are smaller than control clones. In contrast, clones that originate in type I clones are similar in size to controls.

(San-Juán and Baonza, 2011)

Strongly feminizes triploid intersexes. Degree of feminization of the foreleg is unusually high (35% of forelegs are entirely female). May be cell lethal in germ-line clones.

(Barbash and Cline, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dpn⁷/dpn¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by pros[+]/pros^V17

(Zhu et al., 2012)

Suppressor of

Statement

Reference

dpn⁷/dpn[+] is a suppressor of lethal | female phenotype of da¹

(Barbash and Cline, 1995)

dpn⁷/dpn[+] is a suppressor of lethal | female phenotype of sc^sisB-1

(Barbash and Cline, 1995)

dpn⁷/dpn[+] is a suppressor of lethal | female phenotype of sisA¹

(Barbash and Cline, 1995)

NOT Suppressor of

Statement

Reference

dpn⁷ is a non-suppressor of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

dpn⁷ is a non-suppressor of increased cell number | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

dpn⁷ has type II neuroblast | larval stage phenotype, non-enhanceable by N[+]/N^55e11

(Zhu et al., 2012)

Suppressed by

Statement

Reference

dpn⁷/dpn¹ has type I neuroblast | third instar larval stage phenotype, suppressible | partially by pros[+]/pros^V17

(Zhu et al., 2012)

NOT suppressed by

Statement

Reference

dpn⁷ has type II neuroblast | larval stage phenotype, non-suppressible by N[+]/N^55e11

(Zhu et al., 2012)

NOT Suppressor of

Statement

Reference

dpn⁷ is a non-suppressor of type II neuroblast | ectopic | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

pros^V17/+ partially rescues the reduction in number of type I neuroblasts which is seen in the brains of late third larval instar dpn¹/dpn⁷ larvae.

The decrease in the number of type II neuroblasts in the brain that is seen in dpn⁷/+ larvae is unaffected if the animals are also heterozygous for N^55e11.

(Zhu et al., 2012)

dpn⁷ does not suppress the ectopic neuroblast phenotype seen when clones expressing N^{int.G.Scer\UAS} are induced under the control of Scer\GAL4^αTub84B.PL.

(San-Juán and Baonza, 2011)

dpn-mediated suppression of sisA female lethality is entirely zygotic.

(Barbash and Cline, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Shows complex complementation behavior. Complementation between mutant dpn alleles reveals differences in strength but little male-specific lethality.

(Barbash and Cline, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dpn⁷

(Li et al., 2017, Zacharioudaki et al., 2016, Córdoba and Estella, 2014, Zhu et al., 2012, Kappes et al., 2011, San-Juán and Baonza, 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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