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General Information
Symbol
Dmel\rushUAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0267094
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS drives expression of rush tagged at the C-terminus with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of rushScer\UAS.T:Avic\GFP using Scer\GAL4CU1 causes an increase in the size of Rab7-positive endosomes, and the formation of large vesicle clusters as judged by GFP fluorescence.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

While Df(1)rush4, sta+t4.4 homozygous fies and Rab52heterozygotes are viable, their combination decreases viability at embryonic and pupal stages. This decreased survival is partially rescued by expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS.

While HrsD28 homozygotes die at the early pupal stage, animals homozygous for HrsD28 and Df(1)rush4, sta+t4.4 never reach the pupal stage. Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS rescues this early lethality.

Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS decreases the fraction of car1 flies eclosing.

Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS decreases the fraction of VhaSFDEY04644/+ flies eclosing.

Co-expression of rushScer\UAS.T:Avic\GFP and Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP using Scer\GAL4CU1 results in striking changes in endosome morphology: the Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP-induced large endosomes change their shape and form clusters of smaller interconnected vesicles.

Scer\GAL4arm.PS-mediated expression of rushScer\UAS.T:Avic\GFP decreases the viability of GdiAF160 heterozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
rushScer\UAS.T:Avic\GFP
rushUAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (1)