FlyBase Allele Report: Dmel\Rho1[72F]

General Information

Symbol

Dmel\Rho1^72F

Species

D. melanogaster

Name

FlyBase ID

FBal0061663

Feature type

allele

Associated gene

Dmel\Rho1

Associated Insertion(s)

Carried in Construct

Also Known As

RhoA^72F

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Strutt et al., 1997)

Progenitor genotype

Rho1^k07236

(Strutt et al., 1997)

Cytology

Description

Imprecise excision of the P{lacW} element, deleting part of the coding region, including the translation start site.

(Strutt et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by scrib¹

(Kong et al., 2022)

ameliorates neurodegenerative disease

modeled by SNF4Aγ^loe

(Cook et al., 2012)

exacerbates hereditary systemic amyloidosis 1

modeled by Hsap\TTR^{V30M.UAS.Tag:HA}

(Oliveira da Silva et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adherens junction | somatic clone

(Warner and Longmore, 2009)

embryonic/larval tracheal dorsal trunk (with Rho1^72O)

(Chung et al., 2009)

embryonic/larval tracheal section

(Matusek et al., 2006)

embryonic dorsal epidermis

(Strutt et al., 1997)

embryonic dorsal epidermis | anterior

(Strutt et al., 1997)

head

(Strutt et al., 1997)

Detailed Description

Statement

Reference

Heterozygous Rho1^72F flies do not show significant neural vacuolization after 5 days as adults.

Heterozygous Rho1^72F flies perform well in a fast phototaxis assay.

(Cook et al., 2012)

Rho1^72F/Rho1^72O stage 16 embryos have longer dorsal trunks than normal.

(Chung et al., 2009)

F-actin localisation at the level of the adherens junctions is disrupted in Rho1^72F homozygous clones.

(Warner and Longmore, 2009)

Homozygous Rho1^72F mutants exhibit CNS defects in less than 10% of embryos.

(Matusek et al., 2008)

The Rho1^72F mutation arrests tracheal development before dorsal trunk fusion takes place.

(Matusek et al., 2006)

Rho1^72F/Rho1^72O, Rho1^72F/Rho1^72R and Rho1^72R/Rho1^72O embryos have an "anterior open" phenotype; the epidermis fails to close in the dorsal/anterior region. Rarely, the dorsal epidermis also fails to close. Some head structures are missing.

(Strutt et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rho1^72F is an enhancer of visible | adult stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F is an enhancer of abnormal neuroanatomy phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F is an enhancer of partially lethal - majority die phenotype of DAAM^Ex1

(Matusek et al., 2006)

NOT Enhancer of

Statement

Reference

Rho1^72F/Rho1[+] is a non-enhancer of abnormal neuroanatomy phenotype of DAAM^Ex1

(Gombos et al., 2015)

Rho1^72F/Rho1[+] is a non-enhancer of visible | recessive phenotype of Rab23⁵¹

(Pataki et al., 2010)

Suppressor of

Statement

Reference

Rho1^72F/Rho1[+] is a suppressor of abnormal phototaxis phenotype of SNF4Aγ^loe

(Cook et al., 2012)

Rho1^72F/Rho1[+] is a suppressor of abnormal neuroanatomy phenotype of SNF4Aγ^loe

(Cook et al., 2012)

NOT Suppressor of

Statement

Reference

Rho1^72F/Rho1[+] is a non-suppressor of visible | recessive phenotype of Rab23⁵¹

(Pataki et al., 2010)

Other

Statement

Reference

Rho1^72F/Rho1[+], SNF4Aγ^loe has long lived phenotype

(Cook et al., 2012)

Rho1^72F/Rho1[+], Sb^63b has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^72F/Rho1[+], Sb⁷⁰ has visible | dominant phenotype

(Bayer et al., 2003)

Df(3R)sbd105/+, Rho1^72F has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^72F, Sb^63b/Sb[+] has visible phenotype

(Bayer et al., 2003)

Rho1^72F, Sb[+]/Sb⁷⁰ has visible phenotype

(Bayer et al., 2003)

Rho1^72F, zip[+]/zip^Ebr has visible | dominant phenotype

(Halsell et al., 2000)

Rho1^72F, zip^Ebr has visible | dominant phenotype

(Halsell et al., 2000)

Df(2R)Jp1/+, Rho1^72F has visible | dominant phenotype

(Halsell et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rho1^72F has adherens junction | somatic clone phenotype, enhanceable by Rab11^{S25N.UASp.YFP}/Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

Suppressed by

Statement

Reference

Rho1^72F has adherens junction | somatic clone phenotype, suppressible by Scer\GAL4^Act5C.PI/Cdc42^RNAi.UAS

(Warner and Longmore, 2009)

Rho1^72F has adherens junction | somatic clone phenotype, suppressible by Scer\GAL4^Act5C.PI/Rab5^{S43N.UASp.YFP}

(Warner and Longmore, 2009)

Rho1^72F has adherens junction | somatic clone phenotype, suppressible by Rab5^GD10492/Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

NOT suppressed by

Statement

Reference

Rho1^72F has adherens junction | somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/dia^{CA.UAS.Tag:HA}

(Warner and Longmore, 2009)

Rho1^72F has adherens junction | somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Rab5^{Q88L.UASp.YFP}

(Warner and Longmore, 2009)

Enhancer of

Statement

Reference

Rho1^72F is an enhancer of eye phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F is an enhancer of eye disc | third instar larval stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F is an enhancer of axon | third instar larval stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F is an enhancer of larval brain | third instar larval stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rho1^72F/Rho1[+] is an enhancer of retina phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Rho1^72F/Rho1[+] is an enhancer of ommatidium phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Rho1^72F is an enhancer of connective phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F is an enhancer of lateral longitudinal fascicle phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F is an enhancer of embryo phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F is an enhancer of neuropil phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F is an enhancer of fascicle phenotype of DAAM^Ex68

(Matusek et al., 2008)

Rho1^72F/Rho1[+] is an enhancer of retina | pupal stage P7 phenotype of Scer\GAL4^GMR.PF, tkv^RNAi.IR1.UAS, tkv^RNAi.IR2.UAS

(Cordero et al., 2007)

Rho1^72F/Rho1[+] is an enhancer of wing vein phenotype of Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS

(Cordero et al., 2007)

Rho1^72F/Rho1[+] is an enhancer of interommatidial precursor cell | pupal stage P7 phenotype of Scer\GAL4^GMR.PF, tkv^RNAi.IR2.UAS/tkv^RNAi.IR1.UAS

(Cordero et al., 2007)

Rho1^72F is an enhancer of embryonic/larval tracheal section phenotype of DAAM^Ex1

(Matusek et al., 2006)

NOT Enhancer of

Statement

Reference

Rho1^72F/Rho1[+] is a non-enhancer of adult mushroom body alpha-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Rho1^72F/Rho1[+] is a non-enhancer of adult mushroom body beta-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Rho1^72F/Rho1[+] is a non-enhancer of wing hair phenotype of Rab23⁵¹

(Pataki et al., 2010)

Suppressor of

Statement

Reference

Rho1^72F/Rho1[+] is a suppressor of vacuole phenotype of SNF4Aγ^loe

(Cook et al., 2012)

NOT Suppressor of

Statement

Reference

Rho1^72F/Rho1[+] is a non-suppressor of wing hair phenotype of Rab23⁵¹

(Pataki et al., 2010)

Rho1^72F is a non-suppressor of embryonic/larval tracheal section phenotype of DAAM^C.UASp, Scer\GAL4^btl.PS

(Matusek et al., 2006)

Other

Statement

Reference

Rho1^72F, Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

Rho1^72F/Rho1[+], Sb^63b has leg phenotype

(Bayer et al., 2003)

Rho1^72F/Rho1[+], Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

Df(3R)sbd105/+, Rho1^72F has leg phenotype

(Bayer et al., 2003)

Rho1^72F, Sb^63b has leg phenotype

(Bayer et al., 2003)

Rho1^72F, zip[+]/zip^Ebr has wing phenotype

(Halsell et al., 2000)

Rho1^72F, zip^Ebr has wing phenotype

(Halsell et al., 2000)

Rho1^72F, zip^Ebr has leg phenotype

(Halsell et al., 2000)

Rho1^72F, zip^Ebr has wing vein phenotype

(Halsell et al., 2000)

Rho1^72F, zip[+]/zip^Ebr has leg phenotype

(Halsell et al., 2000)

Rho1^72F, zip[+]/zip^Ebr has wing vein phenotype

(Halsell et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Rho1^72F does not enhance the axonal growth defects seen in the α and β lobes of DAAM^Ex1 mutant mushroom bodies.

(Gombos et al., 2015)

Rho1^72F heterozygosity increases the performance index of SNF4Aγ^loe flies in a fast phototaxis assay.

Rho1^72F/+; SNF4Aγ^loe/SNF4Aγ^loe flies show a significant increase in lifespan compared to SNF4Aγ^loe mutants.

A heterozygous Rho1^72F background suppresses the neuronal vacuolization seen in SNF4Aγ^loe mutants to approximately half the level in single mutants. The number of vacuoles is also reduced, compared to SNF4Aγ^loe single mutants.

(Cook et al., 2012)

Heterozygosity for Rho1^72F enhances the patterning defects in the retina of flies co-expressing Arf51F^GD13822 with Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF.

(Johnson et al., 2011)

F-actin projections in the posterior region of Rho1^72F/+ ; RpII140^wimp/+ stage 10b oocytes are reduced compared to wild-type oocytes.

(Tanaka et al., 2011)

Rho1^72F does not exhibit dominant genetic interaction with Rab23⁵¹.

(Pataki et al., 2010)

Rho1^72F clones expressing dia^{CA.Scer\UAS.T:Ivir\HA1} still exhibit disrupted adherens junctions.

Expression of Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP} in Rho1^72F clones enhances the Rho1 mutant phenotype. In addition to frequent disruptions to adherens junctions between clonal cells, there is also disruption of adherens junctions between clonal and wild-type cells.

Cdc42^{dsRNA.Scer\UAS} expression suppresses the adherens junctions defects observed between two Rho1^72F cells but does not suppress the increased apical area.

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} in Rho1^72F clones suppresses the adherens junction defect seen between two Rho1^72F cells, although decreased apical tension is not affected.

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} (under the control of Scer\GAL4^Act5C.PI) in Rho1^72F clones does not worsen the Rho1^72F adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype that is not found in Rho1^72O clones.

Expression of Rab5^GD10492 in Rho1^72F clones suppresses the adherens junction defect seen between two Rho1^72F cells, although decreased apical tension is not affected.

(Warner and Longmore, 2009)

A Rho1^72F heterozygous background weakly enhances the zygotic DAAM^Ex68 CNS phenotype.

(Matusek et al., 2008)

The frequency of inter-ommatidial pattering defects in tkv^IR1.Scer\UAS; tkv^IR2.Scer\UAS; Scer\GAL4^GMR.PF pupal retinas at 42 hours APF is significantly enhanced by Rho1^72F/+.

The wing vein thickening phenotype seen in tkv^IR2.Scer\UAS; Scer\GAL4^sd-SG29.1 adults is enhanced by Rho1^72F/+.

(Cordero et al., 2007)

The tracheal cuticle and semi-lethal phenotypes of DAAM^Ex1 larvae is enhanced by Rho1^72F.

Rho1^72F does not modify the effect of Scer\GAL4^btl.PS>DAAM^{C.Scer\UAS.P\T} expression on the larval trachea.

(Matusek et al., 2006)

Rho1^72F shows a strong interaction (at least 50% of double heterozygotes have at least one malformed leg) with the following mutations: Sb^63b and Sb⁷⁰. Rho1^72F shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: Df(3R)sbd105.

(Bayer et al., 2003)

96% of Rho1^72F/zip^Ebr double heterozygotes have a malformed leg phenotype. Wing defects are also seen. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In more severe cases, wings lacking adhesion between the wing blade bilayer and any apparent wing venation are seen. 17% of Rho1^72F/Df(2R)Jp1 double heterozygotes show a malformed phenotype.

(Halsell et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rho1^E3.10

(Halsell et al., 2000)

Rho1^J3.8

(Halsell et al., 2000)

Rescued by

Rho1^72F is rescued by Scer\GAL4^Act5C.PI/Rho1^UAS.cMa

(Warner and Longmore, 2009)

Comments

Rho1^72F clones are rescued by expression of Rho1^Scer\UAS.cMa. In some cases, a decrease in the apical area is observed, perhaps due to the high level of Rho1^Scer\UAS.cMa overexpression.

(Warner and Longmore, 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7326

y¹ w^*; Rho1^72F/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Rho1^72F

(Kong et al., 2022, Dunn et al., 2018, Hernandez-Fleming et al., 2017, Tanaka et al., 2011, Warner and Longmore, 2009, Xu et al., 2008, Cordero et al., 2007)

Rho1^72f

(Johnson et al., 2011)

Rho^72F

(Cook et al., 2012)

Rho^A72F

(Pataki et al., 2010)

RhoA^72F

(Gazsó-Gerhát et al., 2023, Gombos et al., 2015, Matusek et al., 2008, Matusek et al., 2006, Bayer et al., 2003)

rho1⁷²

(Hernandez-Fleming et al., 2017)

rho1^72F

(Oliveira da Silva et al., 2020, Schimizzi et al., 2016, Marchetti et al., 2014, Lee and Harris, 2013)

rhoA^72F

(Chung et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (23)

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