FB2025_01 , released February 20, 2025
Allele: Dmel\diaCA.UAS.Tag:HA
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General Information
Symbol
Dmel\diaCA.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0159328
Feature type
allele
Associated gene
Dmel\dia
Associated Insertion(s)
Carried in Construct
P{UAS-dia.CA}
Also Known As
UAS-diaCA, UAS-Dia-CA, UAS-diaCA, DiaCA, UAS-diaphanousCA, UAS-diaCA NC
Key Links
Transgenic product class
inframe_deletion
(Somogyi and Rorth, 2004)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Somogyi and Rorth, 2004)
Mutagen
in vitro construct
(Somogyi and Rorth, 2004)
Progenitor genotype
Carried in construct
P{UAS-dia.CA}
Cytology
Description

UASt regulatory sequences drive expression of dia cDNA SD13607 in which the N-terminal 449 amino acids have been replaced by a short sequence encoding three Tag:HA tags, and the C-terminal amino acids 1029-1091 (the predicted autoinhibitory domain) have been removed. Encodes an activated form of dia.

(Somogyi and Rorth, 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dia
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  autosomal dominant auditory neuropathy 1
      CEA
      (Schoen et al., 2010)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adherens junction | adult stage, with Scer\GAL4GMR.PU

      (Rosa-Birriel et al., 2024)

      amnioserosa, with Scer\GAL4c381

      (Homem and Peifer, 2008)

      amnioserosa, with Scer\GAL4en-e16E

      (Homem and Peifer, 2008)

      border follicle cell, with Scer\GAL4slbo.2.6

      (Somogyi and Rorth, 2004)

      cone cell | adult stage, with Scer\GAL4GMR.PU

      (Rosa-Birriel et al., 2024)

      connective, with Scer\GAL4elav-C155

      (Matusek et al., 2008)

      ectoderm | extended germ band stage

      (Bertet et al., 2009)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL4btl.PS

      (Matusek et al., 2006)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL4en-e16E

      (Laplante et al., 2010)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL4prd.RG1

      (Laplante et al., 2010)

      embryonic epidermis, with Scer\GAL4en-e16E

      (Laplante et al., 2010, Homem and Peifer, 2008)

      embryonic epidermis, with Scer\GAL4prd.RG1

      (Laplante et al., 2010, Homem and Peifer, 2008)

      epithelial cell | somatic clone, with Scer\GAL4Act5C.PI

      (Warner and Longmore, 2009)

      eye, with Scer\GAL4GMR.PU

      (Rosa-Birriel et al., 2024, Sansores-Garcia et al., 2011)

      filamentous actin, with Scer\GAL4slbo.2.6

      (Poukkula et al., 2014)

      filamentous actin | adult stage, with Scer\GAL4GMR.PU

      (Rosa-Birriel et al., 2024)

      leg disc, with Scer\GAL4dpp.PU

      (Sansores-Garcia et al., 2011)

      nucleus | larval stage, with Scer\GAL430A

      (Sansores-Garcia et al., 2011)

      ommatidium | adult stage, with Scer\GAL4GMR.PU

      (Rosa-Birriel et al., 2024)

      organism | dorsal closure stage, with Scer\GAL4c381

      (Homem and Peifer, 2008)

      presumptive embryonic/larval central nervous system, with Scer\GAL4elav-C155

      (Matusek et al., 2008)

      salivary gland body primordium | embryonic stage 11, with Scer\GAL4fkh.PH

      (Chung et al., 2017)

      salivary gland body primordium | embryonic stage 14, with Scer\GAL4fkh.PH

      (Chung et al., 2017)

      symmetrical commissure, with Scer\GAL4elav-C155

      (Matusek et al., 2008)

      tracheal fusion cell, with Scer\GAL4btl.PS

      (Matusek et al., 2006)

      wing disc, with Scer\GAL430A

      (Sansores-Garcia et al., 2011)

      wing disc, with Scer\GAL4dpp.PU

      (Sansores-Garcia et al., 2011)

      wing hinge primordium, with Scer\GAL430A

      (Sansores-Garcia et al., 2011)
      Detailed Description
      Statement
      Reference

      Salivary gland primordia expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4fkh.PH still form an invagination pit during stage 11 of embryogenesis, although much wider, but fail to form a supra-cellular myosin cable, exhibit significantly decreased circularity, and their cells apically expand instead of constricting during invagination, as compared to controls; the region to the anterior of the presumptive invagination site fails to cluster the primordium cells with lower apical surface area, and these cells form only small- and medium-sized apical foci of Rok protein but no apical foci of myosin, as compared to controls. These diaCA.Scer\UAS.T:Ivir\HA1-expressing salivary gland primordia exhibit a significant delay in internalization and migration by stage 14 of embryogenesis, as compared to controls.

      (Chung et al., 2017)

      Expression via Scer\GAL4slbo.2.6 leads to the appearance of F-actin-rich foci in cells.

      (Poukkula et al., 2014)

      Expression of diaCA.UAS.Tag:HA under the control of Scer\GAL4dpp.PU or Scer\GAL430A results in overgrowth of the expressing region of the wing disc; overgrowth is characterised by increased proliferation (BrdU incorporation) and extra folding of the disc. Expression of diaCA.UAS.Tag:HA under the control of Scer\GAL430A causes overgrowth of the presumptive wing hinge region, where some expressing cells leave the epithelium and have abnormal DAPI staining; cells that remain in the epithelium have similar polarity to non-expressing cells. Expression of diaCA.UAS.Tag:HA under the control of Scer\GAL4dpp.PU also increases the size of the leg disc.

      Expression of diaCA.UAS.Tag:HA under the control of Scer\GAL4GMR.PU causes lethality prior to eclosion; escapers have severe defects in eye morphology.

      (Sansores-Garcia et al., 2011)

      Embryos expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4en-e16E or Scer\GAL4prd.RG1 have deep epidermal grooves. The epidermis appears to impinge upon the dorsal trunk in these embryos and the underlying trachea are more convoluted than wild type. No breaks in the tracheal lateral trunk are seen in these embryos.

      (Laplante et al., 2010)

      Flies expressing diaCA.Scer\UAS.T:Ivir\HA1 in the Johnston's organ under the control of Scer\GAL4ph-p-NP1046, Scer\GAL4CG13795-NP1245, Scer\GAL4NP6303 or Scer\GAL4JO15 display an impaired response to sound. A significant reduction in sound-evoked potentials compared with wild-type and parental controls is observed in these animals.

      (Schoen et al., 2010)

      Expression of diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in a collapse of the epithelium, and the apical cell surfaces are reduced in size in embryos at germ-band extension.

      (Bertet et al., 2009)

      Expression of diaCA.Scer\UAS.T:Ivir\HA1 in pupal eye MARCM clones does not affect the adherens junctions. diaCA.Scer\UAS.T:Ivir\HA1 expressing cells develop a rounded morphology, especially primary pigment epithelial cells.

      (Warner and Longmore, 2009)

      Expression of diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E in individual amnioserosa cells induces premature apical constriction in these cells, and neighbouring wild-type cells become elongated. Cortical F-actin is increased in the cells that express diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E.

      Expression of diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c381 in all amnioserosal cells results in all the cells becoming apically smaller and uniformly round, suggesting that they all attempt to constrict simultaneously. Cortical F-actin is increased in the amnioserosa cells. The network of apical myosin filaments that is normally seen in wild-type cells become reorganised into stress-fibre-like bundles parallel to the epithelium.

      Dorsal closure is substantially slowed in embryos expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c381 compared to wild type. Most of the embryos do complete dorsal closure, but have defects in cell-cell matching as the two epidermal sheets meet.

      Segmental grooves persist much longer than normal in embryos expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4en-e16E or Scer\GAL4prd.RG1 and become very deep. Epidermal cells expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4en-e16E are wider in the anterior/posterior axis and shorter in the dorsal/ventral axis than neighbouring wild-type cells.

      Epidermal cells expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E have elevated cortical F-actin levels compared to wild type.

      Epidermal cells expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4prd.RG1 have higher levels of cortical myosin compared to wild-type neighbouring cells, with myosin being especially enriched at the dorsal/ventral cell borders.

      (Homem and Peifer, 2008)

      Expression of diaCA.Scer\UAS.T:Ivir\HA1 pan-neuronally under the control of Scer\GAL4elav-C155 results in disruption of CNS fasciculation and thinning of commissures and breaks in connectives can be observed.

      (Matusek et al., 2008)

      Overexpression of diaCA.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4btl.PS, leads to fusion defects in the dorsal trunk, constrictions at the fusion cells, widening at other areas of the dorsal trunk and impairments of terminal branch differentiation.

      (Matusek et al., 2006)

      Border cell migration is blocked by expression of diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4slbo.2.6.

      (Somogyi and Rorth, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4en-e16E, diaCA.UAS.Tag:HA has embryonic epidermis phenotype, non-suppressible by zip1/zip1

      (Homem and Peifer, 2008)

      Scer\GAL4c381, diaCA.UAS.Tag:HA has amnioserosa phenotype, non-suppressible by zip1/zip1

      (Homem and Peifer, 2008)
      NOT Enhancer of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Rho172F clones expressing diaCA.Scer\UAS.T:Ivir\HA1 still exhibit disrupted adherens junctions.

      Rho172O clones expressing diaCA.Scer\UAS.T:Ivir\HA1 still exhibit disrupted adherens junctions.

      (Warner and Longmore, 2009)

      The cell shape changes that are seen in epidermal cells of embryos expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E are still seen in a zip1 homozygous background.

      The cell shape changes that are seen in amnioserosa cells of embryos expressing diaCA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c381 are still seen in a zip1 homozygous background.

      (Homem and Peifer, 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      DiaCA
      (Matusek et al., 2006)
      diaCA.Scer\UAS.T:Ivir\HA1
      diaCA.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (20)